GhPLP2 Positively Regulates Cotton Resistance to Verticillium Wilt by Modulating Fatty Acid Accumulation and Jasmonic Acid Signaling Pathway

Zhu, Yutao; Hu, Xinning; Wang, Ping; Gao, Linying; Pei, Yakun; Ge, Zhaoyue; Ge, Xiaoyang; Li, Fuguang; Hou, Yuxia

doi:10.3389/fpls.2021.749630

Cited by 20 publications

(5 citation statements)

References 110 publications

(153 reference statements)

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“…Especially, α-linolenic acid synthesis was downregulated. Linolenic acid was the precursor of jasmonic acid (JA), and JA is a lipogenic plant hormone that regulates the defensive responses of plants to biological and abiotic stresses (Zhao et al, 2014;Balfagon et al, 2019;Zhu et al, 2021;Pyo et al, 2022). Downregulated linolenic acid may suggest that stress levels in potato plants of group CC1 were not increased very much compared to that of group CC0.…”

Section: Metabolic Pathways In Potato Controlling Root Exudates Affec...mentioning

confidence: 99%

Continuous cropping of potato changed the metabolic pathway of root exudates to drive rhizosphere microflora

Xing,

Zhang,

Zhang

et al. 2024

Front. Microbiol.

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For potato production, continuous cropping (CC) could lead to autotoxicity buildup and microflora imbalance in the field soil, which may result in failure of crops and reduction in yield. In this study, non-targeted metabolomics (via liquid chromatography with tandem mass spectrometry (LC–MS/MS)) combined with metagenomic profiling (via high-throughput amplicon sequencing) were used to evaluate correlations between metabolomics of potato root exudates and communities of bacteria and fungi around potato plants to illustrate the impacts of CC. Potato plants were grown in soil collected from fields with various CC years (0, 1, 4, and 7 years). Metabolomic analysis showed that the contents and types of potential autotoxins in potato root exudates increased significantly in CC4 and CC7 plants (i.e., grown in soils with 4 and 7 years of CC). The differentially expressed metabolites were mainly produced via alpha-linolenic acid metabolism in plant groups CC0 and CC1 (i.e., no CC or 1 year CC). The metabolomics of the groups CC4 and CC7 became dominated by styrene degradation, biosynthesis of siderophore group non-ribosomal peptides, phenylpropanoid biosynthesis, and biosynthesis of various plant secondary metabolites. Continuous cropping beyond 4 years significantly changed the bacterial and fungal communities in the soil around the potato crops, with significant reduction of beneficial bacteria and accumulation of harmful fungi. Correlations between DEMs and microflora biomarkers were established with strong significances. These results suggested that continuous cropping of potato crops changed their metabolism as reflected in the plant root exudates and drove rhizosphere microflora to directions less favorable to plant growth, and it needs to be well managed to assure potato yield.

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Section: Metabolic Pathways In Potato Controlling Root Exudates Affec...mentioning

confidence: 99%

Continuous cropping of potato changed the metabolic pathway of root exudates to drive rhizosphere microflora

Xing,

Zhang,

Zhang

et al. 2024

Front. Microbiol.

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“…In recent years, efforts have been made to manipulate key fatty acid synthetic genes in various species using transgenic technology [43][44][45][46][47]. Transformations using transgenes encoding key enzymes or enzyme subunits have resulted in the alteration in lipid levels to varying degrees [37,48], and in some cases, the oil content was reduced [49]. Four key enzymes were involved in the fatty acid carbon-chain extension process, namely KAS, KAR, HAD, and ENR.…”

Section: The Application Of Fatty Acid Synthase In Improving Seed Oil...mentioning

confidence: 99%

Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars

Liu,

Wang,

Hua

et al. 2023

Plants

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Three carbon-chain extension genes associated with fatty acid synthesis in upland cotton (Gossypium hirsutum), namely GhKAR, GhHAD, and GhENR, play important roles in oil accumulation in cotton seeds. In the present study, these three genes were cloned and characterized. The expression patterns of GhKAR, GhHAD, and GhENR in the high seed oil content cultivars 10H1014 and 10H1041 differed somewhat compared with those of 10H1007 and 2074B with low seed oil content at different stages of seed development. GhKAR showed all three cultivars showed higher transcript levels than that of 2074B at 10-, 40-, and 45-days post anthesis (DPA). The expression pattern of GhHAD showed a lower transcript level than that of 2074B at both 10 and 30 DPA but a higher transcript level than that of 2074B at 40 DPA. GhENR showed a lower transcript level than that of 2074B at both 15 and 30 DPA. The highest transcript levels of GhKAR and GhENR were detected at 15 DPA in 10H1007, 10H1014, and 10H1041 compared with 2074B. From 5 to 45 DPA cotton seed, the oil content accumulated continuously in the developing seed. Oil accumulation reached a peak between 40 DPA and 45 DPA and slightly decreased in mature seed. In addition, GhKAR and GhENR showed different expression patterns in fiber and ovule development processes, in which they showed high expression levels at 20 DPA during the fiber elongation stage, but their expression level peaked at 15 DPA during ovule development processes. These two genes showed the lowest expression levels at the late seed maturation stage, while GhHAD showed a peak of 10 DPA in fiber development. Compared to 2074B, the oil contents of GhKAR and GhENR overexpression lines increased 1.05~1.08 folds. These results indicated that GhHAD, GhENR, and GhKAR were involved in both seed oil synthesis and fiber elongation with dual biological functions in cotton.

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“…In our study, transcriptome analysis revealed that a significant number of DEGs was enriched in the "plant hormone signaling" pathway when comparing non-treated and salt-treated RIL pur-W22 and RIL bro-W22 seedlings. Additionally, through KEGG pathway enrichment analysis, we observed the enrichment of precursor synthesis or metabolic pathways related to various plant hormones [48][49][50][51]. For instance, the "tryptophan biosynthesis" pathway is associated with auxin, the "methionine metabolism" pathway corresponds to ethylene, the "carotenoid synthesis" pathway is related to abscisic acid (ABA), and the "linoleic acid metabolism" pathway is correlated with jasmonic acid (Jas) (Figure S3).…”

Section: The Salt Stress Tolerance In Maize Seedlings May Be Mediated...mentioning

confidence: 99%

Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings

Wang

Yuan

et al. 2023

Plants

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Anthocyanin, a kind of flavonoid, plays a crucial role in plant resistance to abiotic stress. Salt stress is a kind of abiotic stress that can damage the growth and development of plant seedlings. However, limited research has been conducted on the involvement of maize seedlings in salt stress resistance via anthocyanin accumulation, and its potential molecular mechanism is still unclear. Therefore, it is of great significance for the normal growth and development of maize seedlings to explore the potential molecular mechanism of anthocyanin improving salt tolerance of seedlings via transcriptome analysis. In this study, we identified two W22 inbred lines (tolerant line pur–W22 and sensitive line bro–W22) exhibiting differential tolerance to salt stress during seedling growth and development but showing no significant differences in seedling characteristics under non–treatment conditions. In order to identify the specific genes involved in seedlings’ salt stress response, we generated two recombinant inbred lines (RILpur–W22 and RILbro–W22) by crossing pur–W22 and bro–W22, and then performed transcriptome analysis on seedlings grown under both non–treatment and salt treatment conditions. A total of 6100 and 5710 differentially expressed genes (DEGs) were identified in RILpur–W22 and RILbro–W22 seedlings, respectively, under salt–stressed conditions when compared to the non–treated groups. Among these DEGs, 3160 were identified as being present in both RILpur–W22 and RILbro–W22, and these served as commonly stressed EDGs that were mainly enriched in the redox process, the monomer metabolic process, catalytic activity, the plasma membrane, and metabolic process regulation. Furthermore, we detected 1728 specific DEGs in the salt–tolerant RILpur–W22 line that were not detected in the salt–sensitive RILbro–W22 line, of which 887 were upregulated and 841 were downregulated. These DEGs are primarily associated with redox processes, biological regulation, and the plasma membrane. Notably, the anthocyanin synthesis related genes in RILpur–W22 were strongly induced under salt treatment conditions, which was consistented with the salt tolerance phenotype of its seedlings. In summary, the results of the transcriptome analysis not only expanded our understanding of the complex molecular mechanism of anthocyanin in improving the salt tolerance of maize seedlings, but also, the DEGs specifically expressed in the salt–tolerant line (RILpur–W22) provided candidate genes for further genetic analysis.

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GhPLP2 Positively Regulates Cotton Resistance to Verticillium Wilt by Modulating Fatty Acid Accumulation and Jasmonic Acid Signaling Pathway

Cited by 20 publications

References 110 publications

Continuous cropping of potato changed the metabolic pathway of root exudates to drive rhizosphere microflora

Continuous cropping of potato changed the metabolic pathway of root exudates to drive rhizosphere microflora

Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars

Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings

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