Ghrelin and NUCB2/Nesfatin‐1 Co‐Localization With Digestive Enzymes in the Intestine of Pejerrey (<scp><i>Odontesthes bonariensis</i></scp>)

Bertucci, Juan Ignacio; Blanco, Ayelén Melisa; Sánchez-Bretaño, Aída; Unniappan, Suraj; Canosa, Luis Fabián

doi:10.1002/ar.24012

Cited by 7 publications

(4 citation statements)

References 78 publications

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“…Data are the mean ± activity was found in early and late pre-juveniles. This suggests that this activity could correspond to a sucrase-independent maltaseglucoamylase complex (Bertucci et al, 2019;Brun et al, 2020;Chaudet et al, 2019;Dhital et al, 2013;Morelos-Castro et al, 2020).…”

Section: Discussionmentioning

confidence: 93%

Understanding the early ontogenetic stages of Mugil liza (Mugilidae): Morphological traits and digestive/metabolic profile of pre‐juveniles after recruitment

Albanesi

González‐Castro

López-Mañanes

2020

Journal of Fish Biology

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The family Mugilidae consists mainly of diadromous species, whose reproduction occurs in offshore waters. Pre-juveniles shift their diet in the surf zone (zooplanktophagous to iliophagous). Later, during their recruitment into estuaries, huge changes take place in their digestive system. However, digestive and metabolic characteristics and some morphological traits at recruitment are unknown for Mugilidae. We performed comparative studies on early and late pre-juveniles of Mugil liza recruited in Mar Chiquita Coastal Lagoon (37 32 0-37 45 0 S, 57 19 0-57 26 0 W, Argentina). We determined digestive enzyme activities (intestine), energy reserves (liver/muscle), total/standard length, total weight, intestinal coefficient, hepatosomatic index and retroperitoneal fat. Pre-juveniles exhibited amylase, maltase, sucrase, lipase, trypsin and aminopeptidase-N (APN) activities, which were maintained over a wide range of pH and temperature, and exhibited Michaelis-Menten kinetics. In late pre-juveniles, amylase (422 ± 131 μmol maltose min −1 mgprot −1), sucrase (86 ± 14 mg glucose min −1 mgprot −1), trypsin (84 ± 9 μmoles min −1 mgprot −1) and APN (0.58 ± 0.08 μmoles min −1 mgprot −1) activities were higher (42%, 28%, 35% and 28%, respectively) than in the early stage. Also, the intestinal coefficient was higher in late (3.04) compared to early (2.06) pre-juveniles. Moreover, the liver appeared to be a main site of glycogen and triglyceride storage in late pre-juveniles, muscle being the site of storage in early pre-juveniles, exhibiting higher glycogen, free glucose and protein concentrations (92%, 82%, 32%, respectively). The results suggest that pre-juveniles of M. liza exhibit an adequate digestive battery to perform complete hydrolysis of various dietary substrates, availability of energy reserves and morphological characteristics to support their feeding habit and growth after recruitment. Our results represent an important contribution to knowledge of the ecology and digestive physiology of pre-juveniles of Mugilidae in the wild. K E Y W O R D S digestive enzymes, energy reserves, Mar Chiquita Coastal Lagoon, Mugil liza, pre-juveniles, recruitment Mariano González-Castro and Alejandra López-Mañanes have contributed equally to this study.

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Section: Discussionmentioning

confidence: 93%

Understanding the early ontogenetic stages of Mugil liza (Mugilidae): Morphological traits and digestive/metabolic profile of pre‐juveniles after recruitment

Albanesi

González‐Castro

López-Mañanes

2020

Journal of Fish Biology

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“…Nesfatin-1, a neuronal peptide responsible for regulating food intake and inducing satiety, is expressed in the hypothalamus and central nervous system (Goebel et al 2009 ; Goebel-Stengel et al 2011 ). However, recent studies revealed that NUCB2/nesfatin-1 is not only expressed in the hypothalamus, but also peripheral organs such as the pancreas, stomach, intestines, liver, kidneys, and adipose and reproductive tissues (Schalla and Stengel 2018 ; Bertucci et al 2019 ; Puzio et al 2021 ; Goyal and Dhar 2022 ). We have also demonstrated the expression of NUCB2/nesfatin-1 in the reproductive organs of mice, including ovaries, the uterus, and testes (Kim et al 2011 ; Kim et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…Recent reports demonstrated that nesfatin-1 is also expressed in peripheral organs of humans and rodents, and functions as a regulatory peptide (Dore et al 2017 ). In rats, nesfatin-1 immunoreactivity has been identified in the gastric tract, suggesting that it has a role in gastrointestinal function (Li et al 2013 ; Bertucci et al 2019 ). Nesfatin-1 is expressed in ghrelin-secreting X/A-like cells in the stomach, which stimulate appetite (Stengel et al 2010 ).…”

Section: Introductionmentioning

confidence: 99%

NUCB2/nesfatin-1 suppresses the acrosome reaction in sperm within the mouse epididymis

Kim

Sun

et al. 2023

Animal Cells and Systems

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Nesfatin-1, a polypeptide hormone derived from the nucleobindin 2 (NUCB2) precursor protein, is known to regulate appetite and energy metabolism. Recent studies have also shown that NUCB2/nesfatin-1 is expressed in the reproductive organs of mice. However, the expression and potential role of NUCB2/nesfatin-1 in the mouse epididymis remain unclear. Therefore, we investigated the expression of NUCB2/nesfatin-1 in the mouse epididymis and its potential function. NUCB2/nesfatin-1 was detected in the epididymis by qRT-PCR and western blotting, and high expression levels were observed in epididymal epithelial cells by immunohistochemical staining. Pregnant mare’s serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) injections significantly increased NUCB2/nesfatin-1 expression in the epididymis. After castration, NUCB2/nesfatin-1 expression in the epididymis decreased, but was significantly increased by testosterone injection. Nesfatin-1-binding sites were found in the middle piece of testicular sperm, but were scarcely detected in the sperm head. By contrast, nesfatin-1 binding sites were identified on the sperm head within the epididymis. Furthermore, nesfatin-1 treatment inhibited the acrosome reaction in epididymal sperm. These results suggest that the nesfatin-1 protein produced in the epididymis binds to nesfatin-1 binding sites on the sperm head and plays a role in suppressing the acrosome reaction before ejaculation.

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“…Nesf-1 ir-cells were found in the feeding regulatory nuclei of the hypothalamus and anterior intestine of the goldfish [14] and in the mucosal cellular layer of the anterior gastrointestinal tract in zebrafish [16]. NUCB2/Nesf-1 and ghrelin co-localize in the enteroendocrine cells of the anterior intestine [17] and in the hepatopancreas of goldfish [18], in the gut mucosal cells of zebrafish [16], and in intestinal enteroendocrine cells of pejerry [19]. Furthermore, the anorexigenic role of Nesf-1 seems to also be conserved in goldfish and Ya-fish, where central or peripheral administration of Nesf-1 induces feeding behavior suppression [14,15,17] as well as in Siberian sturgeon, where it acts through the cholecystokinin signal pathway [20,21].…”

Section: Introductionmentioning

confidence: 97%

Ontogenetic Pattern Changes of Nucleobindin-2/Nesfatin-1 in the Brain and Intestinal Bulb of the Short Lived African Turquoise Killifish

Montesano

Felice

Leggieri

et al. 2019

JCM

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Nesfatin-1 (Nesf-1) was identified as an anorexigenic and well conserved molecule in rodents and fish. While tissue distribution of NUCB2 (Nucleobindin 2)/Nesf-1 is discretely known in vertebrates, reports on ontogenetic expression are scarce. Here, we examine the age-related central and peripheral expression of NUCB2/Nesf-1 in the teleost African turquoise killifish Nothobranchius furzeri, a consolidated model organism for aging research. We focused our analysis on brain areas responsible for the regulation of food intake and the rostral intestinal bulb, which is analogous of the mammalian stomach. We hypothesize that in our model, the stomach equivalent structure is the main source of NUCB2 mRNA, displaying higher expression levels than those observed in the brain, mainly during aging. Remarkably, its expression significantly increased in the rostral intestinal bulb compared to the brain, which is likely due to the typical anorexia of aging. When analyzing the pattern of expression, we confirmed the distribution in diencephalic areas involved in food intake regulation at all age stages. Interestingly, in the rostral bulb, NUCB2 mRNA was localized in the lining epithelium of young and old animals, while Nesf-1 immunoreactive cells were distributed in the submucosae. Taken together, our results represent a useful basis for gaining deeper knowledge regarding the mechanisms that regulate food intake during vertebrate aging.

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Ghrelin and NUCB2/Nesfatin‐1 Co‐Localization With Digestive Enzymes in the Intestine of Pejerrey (Odontesthes bonariensis)

Cited by 7 publications

References 78 publications

Understanding the early ontogenetic stages of Mugil liza (Mugilidae): Morphological traits and digestive/metabolic profile of pre‐juveniles after recruitment

Understanding the early ontogenetic stages of Mugil liza (Mugilidae): Morphological traits and digestive/metabolic profile of pre‐juveniles after recruitment

NUCB2/nesfatin-1 suppresses the acrosome reaction in sperm within the mouse epididymis

Ontogenetic Pattern Changes of Nucleobindin-2/Nesfatin-1 in the Brain and Intestinal Bulb of the Short Lived African Turquoise Killifish

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