Ghrelin stimulates angiogenesis in human microvascular endothelial cells: Implications beyond GH release

Li, Aihua; Cheng, Guangming; Zhu, Geng; Tarnawski, Andrzej S.

doi:10.1016/j.bbrc.2006.11.144

Cited by 60 publications

(54 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…36 Aihua's study proposed that increasing effect of angiogenesis of ghrelin is probably via angiogenic growth factors such as VEGF and bFGF (basic fibroblast growth factor) in HMVEC. 37 Our study also showed that angiogenesis, HIF and VEGF were reduced with ghrelin treatment in hypoxic conditions. It seems that ghrelin in hypoxic condition has reduced the angiogenesis through HIF and VEGF pathways.…”

Section: 29supporting

confidence: 51%

Ghrelin Decreases Angiogenesis, HIF-1α and VEGF Protein Levels in Chronic Hypoxia in Lung Tissue of Male Rats

Alipour

Keyhanmanesh

et al. 2015

Adv Pharm Bull

View full text Add to dashboard Cite

Section: 29supporting

confidence: 51%

Ghrelin Decreases Angiogenesis, HIF-1α and VEGF Protein Levels in Chronic Hypoxia in Lung Tissue of Male Rats

Alipour

Keyhanmanesh

et al. 2015

Adv Pharm Bull

View full text Add to dashboard Cite

“…A previous study suggested that ghrelin secreted from saliva may promote the proliferation of oral keratinocytes (Gröschl et al, 2005). Furthermore, treatment of human microvascular endothelial cells with ghrelin increased their proliferation, migration, and angiogenesis (Li et al, 2007). Based on the findings in this study, along with previous reports, as noted above, it is suggested that ghrelin may play a role in the maintenance of gingival mucosal tissue turnover, as well as downregulating the overreaction of innate immune response that involves IL-8-mediated neutrophil recruitment.…”

Section: Discussionmentioning

confidence: 94%

Expression and Possible Immune-regulatory Function of Ghrelin in Oral Epithelium

et al. 2011

View full text Add to dashboard Cite

Originally found in stomach mucosa, ghrelin is a peptide appetite hormone that has been implicated as an immuno-modulatory factor. Ghrelin has also been found in salivary glands and saliva; however, its expression patterns and biological properties in the oral cavity remain unclear. Therefore, we investigated the expression patterns of ghrelin in saliva, gingival crevicular fluid (GCF), and gingival tissue, as well as its in vitro effects on IL-8 production by TNF-α or LPS-stimulated oral epithelial cells. In the clinical samples obtained from 12 healthy volunteers, the concentration of ghrelin in GCF remarkably exceeded that detected in saliva. The expression of ghrelin mRNAs and growth hormone secretagogue (GHS) receptors could be detected in human oral epithelial cells. Immunohistochemical analysis revealed the expression of ghrelin in gingival epithelium, as well as in fibroblasts in the lamina propria. Ghrelin increased intracellular calcium mobilization and cAMP levels in oral epithelial cells, suggesting that ghrelin acts on epithelial cells to induce cell signaling. Furthermore, synthetic ghrelin inhibited the production of IL-8 from TNF-α or LPS-stimulated oral epithelial cells. These results indicate that ghrelin produced in the oral cavity appears to play a regulatory role in innate immune responses to inflammatory infection.

show abstract

“…Li et al found expression of ghrelin and its receptor mRNA in human dermal microvascular endothelial cells (HMVECs) and showed that ghrelin increases these cells proliferation, migration and angiogenesis through activation of ERK2 signaling [164]. Later, the same group suggested that ghrelin is one of the factors responsible for aging-related angiogenesis impairment [165].…”

Section: Ghrelin Angiogenesis and Its Potential Role In Diabetic Retmentioning

confidence: 99%

Diabetes, Ghrelin And Related Peptides: From Pathophysiology To Vasculopathy

Rocha‐Sousa

2012

TOCVJ

View full text Add to dashboard Cite

Ghrelin and obestatin are two different peptides originated from the same gene isolated in the stomach. Numerous actions have been described where these two peptides are implicated in metabolism, appetite regulation, glucose levels regulation, and a wide range of systemic effects. In this article, we summarize (1) the cellular receptors implicated in ghrelin and obestatin effects; (2) the role of ghrelin and obestatin in metabolism and appetite regulation; (3) their role in the glucose homeostasis regulation and its implication in diabetes patho-physiology; (5) the effects of ghrelin and obestatin in regulation of normal angiogenesis and (6) their possible role in the regulation of diabetes-induced pathologic angiogenesis.Keywords: Ghrelin, obestatin, diabetes pathophysiology, appetite regulation, peptides, diabetes vascular complications. GHRELINGhrelin is an acylated, 28-amino-acid peptide that promotes the release of GH in the hypothalamus. It is the natural ligand of the GHSR-1a receptor [1]. For it to act on the GHSR-1a ghrelin has an n-octanoic acid modification on serine 3 residue [2].The ghrelin gene is located in chromosome 3 (3p25-26) [1], contains four preproghrelin-coding exons, and encodes a precursor of 117aa (preproghrelin) with 82% of homology between species [3]. As a result of alternative splicing of this gene, a 27 aa acylated peptide was identified with the same activity potency as ghrelin (des-Gln14-ghrelin) [4]. Another form of ghrelin, des-acyl ghrelin, exists at significant levels in both stomach and blood. This variant lacks the octanoyl chain in serine 3 and represents more than 90% of the circulating peptide [2,4,5]. In plasma, acyl ghrelin levels are 10-20 fmol/ml while total ghrelin levels are 100-150 fmol/ml (including both acyl and non-acyl forms) [6,7]. Several minor forms of ghrelin were described with modifications on the peptide chain or on the acidic chain and are only present in low amounts [4,8]. Some of these are independently produced and regulated and their levels are not directly related to those of ghrelin [3]. Finally, in a posttranslational process, this gene can originate a 23 aa peptide named obestatin that has some different and even opposite actions than ghrelin [9].During adult life ghrelin is synthesized mainly in the gastric oxyntic mucosa in the X/A cells. Ghrelin is also produced in the X/A cells of the intestine and in some others tissues such as the pancreas, kidney, placenta, lymphatics, *Address correspondence to this author at the Department of Physiology, Faculty of Medicine, Alameda Professor Hernâni Monteiro, 4200-319 Porto, Portugal; Tel: +351 225513644; Fax: +351 225513646; E.mail: arsousa@med.up.pt # Both authors had the same contribution to the article gonads, adrenal, thyroid gland, heart, lung, pituitary, hypothalamus, eye [10], human B-and T-lymphocytes, neutrophils [1,[10][11][12], morula, blastocyts and embryos [13]. Ghelardoni observed that ghrelin gene expression and its protein were, in some tissues, dissociated [14].In fetal lif...

show abstract

Ghrelin stimulates angiogenesis in human microvascular endothelial cells: Implications beyond GH release

Cited by 60 publications

References 28 publications

Ghrelin Decreases Angiogenesis, HIF-1α and VEGF Protein Levels in Chronic Hypoxia in Lung Tissue of Male Rats

Ghrelin Decreases Angiogenesis, HIF-1α and VEGF Protein Levels in Chronic Hypoxia in Lung Tissue of Male Rats

Expression and Possible Immune-regulatory Function of Ghrelin in Oral Epithelium

Diabetes, Ghrelin And Related Peptides: From Pathophysiology To Vasculopathy

Contact Info

Product

Resources

About