GhSNAP33, a t-SNARE Protein From Gossypium hirsutum, Mediates Resistance to Verticillium dahliae Infection and Tolerance to Drought Stress

Abstract

Soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins mediate membrane fusion and deliver cargo to specific cellular locations through vesicle trafficking. Synaptosome-associated protein of 25 kDa (SNAP25) is a target membrane SNARE that drives exocytosis by fusing plasma and vesicular membranes. In this study, we isolated GhSNAP33, a gene from cotton (Gossypium hirsutum), encoding a SNAP25-type protein containing glutamine (Q)b- and Qc-SNARE motifs connected by a linker. GhSNAP33 expression was induced by H2O2, salicylic acid, abscisic acid, and polyethylene glycol 6000 treatment and Verticillium dahliae inoculation. Ectopic expression of GhSNAP33 enhanced the tolerance of yeast cells to oxidative and osmotic stresses. Virus-induced gene silencing of GhSNAP33 induced spontaneous cell death and reactive oxygen species accumulation in true leaves at a later stage of cotton development. GhSNAP33-deficient cotton was susceptible to V. dahliae infection, which resulted in severe wilt on leaves, an elevated disease index, enhanced vascular browning and thylose accumulation. Conversely, Arabidopsis plants overexpressing GhSNAP33 showed significant resistance to V. dahliae, with reduced disease index and fungal biomass and elevated expression of PR1 and PR5. Leaves from GhSNAP33-transgenic plants showed increased callose deposition and reduced mycelia growth. Moreover, GhSNAP33 overexpression enhanced drought tolerance in Arabidopsis, accompanied with reduced water loss rate and enhanced expression of DERB2A and RD29A during dehydration. Thus, GhSNAP33 positively mediates plant defense against stress conditions and V. dahliae infection, rendering it a candidate for the generation of stress-resistant engineered cotton.

Physiological Analysis and Proteome Quantification of Alligator Weed Stems in Response to Potassium Deficiency Stress

“…Moreover, annexin functions as a Ca 2+ -permeable channel in the plasma membrane to mediate the radical-activated plasma membrane Ca 2+ - and K + -permeable conductance in root cells [23]. In our study, the TPC1 and annexin proteins were both up-regulated, suggesting that fluxes in the transport of ions from the roots to leaves or post-phloem sugar transport to the root tip help plants rapidly manage stress [24].…”

“…In our study, the decreased abundance of syntaxin-61 could improve KAT1 and KC1 activity to absorb and utilize more K + . Recent reports have suggested that the overexpression of two SNARE proteins resulted in high tolerance to drought and salt stress in two kinds of plants [24,32]. Therefore, the SNARE proteins in different plants have multiple functions in regard to abiotic stress.…”

An expanded role of the SNARE-containing regulon as it relates to the defense process that Glycine max has to Heterodera glycines

“…The work presented here experimentally tests components that would be expected to function within the regulon, but lie outside of the genetically defined apparatus (Humphry et al 2010). The selected genes encoding myosin XI and callose synthase (CS) relate to their previously reported defense roles and how the roles relate to vesicle trafficking (Ellinger et al 2013;Ellinger, Glöckner, et al 2014;Ellinger, Sode, et al 2014;Yang et al 2014;Peremyslov et al 2015;Leslie et al 2016;De Benedictis et al 2018;Sassmann et al 2018;Wang et al 2018). Furthermore, an examination of CS has been performed due to our previous observations that have shown an apparent increase in callose labeling at sites of engineered resistance in transgenic lines overexpressing GmSYP121-1 (GmSYP121-1-OE), leading to a successful defense response while in contrast GmSYP121-1-RNAi transgenic lines exhibit an impaired defense response and an apparent decrease in callose at sites of successful parasitism (Sharma et al 2016).…”

Isolation and characterization of the GbVIP1 gene and response to Verticillium wilt in cotton and tobacco

“…Among these, PR1 is a marker gene of disease-resistance response, and PR1 was also up-regulated by some disease-resistance genes during incubation of Verticillium wilt in cotton (Lu et al 2011). For instance, Arabidopsis plants overexpressing GhSNAP33 and Gbvdr6 showed resistance to V. dahliae with elevated expression of PR1, and endogenous cAMP induced rapid increases of PR1 transcription in plant defense responses against pathogen Verticillium (Yang et al 2017;Jiang et al 2005;Wang et al 2018). It is possible that GbVIP1 might increase resistance to Verticillium wilt in transgenic tobacco by up-regulating expression of PR1.…”