Giant Artificial Ion Channels Formed by Self-Assembled, Cationic Rigid-Rodβ-Barrels

Baumeister, Bodo; Sakai, Naomi; Matile, Stefan

doi:10.1002/1521-3757(20000602)112:11<2031::aid-ange2031>3.0.co;2-w

Cited by 12 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Identical b-barrel expansion has been observed for L2W-mutations 5 and for rigid-rod b-barrel ion channels with internal lysines. 6 Consistent with these findings as well as with molecular models, 2 we thus conclude that internal`c rowding,'' perhaps supported by internal charge repulsion, accounts as well for the expansion observed for the present L2H-mutations leading to hexamer 1.…”

Section: Suprastructurementioning

confidence: 99%

Toward catalytic rigid‐rod β‐barrels: A hexamer with multiple histidines

2001

Self Cite

View full text Add to dashboard Cite

Rigid-rod b-barrels are composed of interdigitating, short, amphiphilic peptide strands that are flanked by stabilizing rigid-rod``staves.'' As a first step toward the construction of catalytic rigidrod b-barrels, we here report synthesis and study of a new barrel designed to comprise alternating leucine and histidine residues at the inner and lysine and glutamate residues at the outer barrel surface. Synthesis of p-octiphenyls with lateral tripeptide strands followed procedures described previously. Barrel formation by programmed assembly of complementary tripeptide-p-octiphenyl rods was monitored by circular dichroism (CD). CD-mixing curves (Job-plots) were consistent with 1:1-stoichiometry. Guanidinium chloride denaturation experiments gave a DG H20 = )1.8 kcal mol )1 with a C 50 = 1.9 M. Size exclusion chromatography suggested quantitative formation of a hexamer. Facile barrel deconstruction by acid and divalent cations demonstrated the presence of internal, nonproximal histidines. Inclusion complex formation with fluorescent guests corroborated internal hydrophobicity of b-barrel hosts and potential for intratoroidal catalysis.

show abstract

Section: Suprastructurementioning

confidence: 99%

Toward catalytic rigid‐rod β‐barrels: A hexamer with multiple histidines

2001

Self Cite

View full text Add to dashboard Cite

show abstract

“…Biological nanotubular complexes, such as the non-selective pore formed by α-hemolysin 2 , and the highly selective channels of ions 3 and water 4 , provide some of the most spectacular examples of supramolecular assemblies with extraordinary structural and functional sophistication. Taking lessons from biological assemblies, the recent integration of molecular design and synthesis with noncovalent forces such as hydrogenbonding, electrostatic, hydrophobic, π − π and ion-π interactions, along with metal-organic architectures and covalent capture of supramolecular assemblies, has made a significant inroad towards producing various synthetic channels and pores, either from organic building blocks or from cyclic peptides [5][6][7][8][9][10][11][12][13] . Many of these synthetic molecular and supramolecular structures exhibit interesting masstransporting properties.…”

mentioning

confidence: 99%

Self-assembling subnanometer pores with unusual mass-transport properties

et al. 2012

View full text Add to dashboard Cite

A long-standing aim in molecular self-assembly is the development of synthetic nanopores capable of mimicking the mass-transport characteristics of biological channels and pores. Here we report a strategy for enforcing the nanotubular assembly of rigid macrocycles in both the solid state and solution based on the interplay of multiple hydrogen-bonding and aromatic π − π stacking interactions. The resultant nanotubes have modifiable surfaces and inner pores of a uniform diameter defined by the constituent macrocycles. The self-assembling hydrophobic nanopores can mediate not only highly selective transmembrane ion transport, unprecedented for a synthetic nanopore, but also highly efficient transmembrane water permeability. These results establish a solid foundation for developing synthetically accessible, robust nanostructured systems with broad applications such as reconstituted mimicry of defined functions solely achieved by biological nanostructures, molecular sensing, and the fabrication of porous materials required for water purification and molecular separations.

show abstract

An Artificial Ion Channel Formed by a Macrocyclic Resorcin[4]arene with Amphiphilic Cholic Acid Ether Groups

Yoshino¹,

Satake²,

Kobuke³

2001

Angew. Chem.

View full text Add to dashboard Cite

Eine Kleinformat‐Doppelschichtmembran inklusive Ionenkanal wurde bei der End‐zu‐End‐Anordnung zweier Moleküle eines Resorc[4]arens erhalten (siehe schematische Darstellung). Die Steroid‐Einheiten bieten den Vorteil, dass die Poren relativ lang geöffnet sind (2.5–4.5 s; im Falle gewöhnlicher Alkylketten dagegen nur wenige zig Millisekunden), und ermöglichen es, die hydrophilen Öffnungen zu modifizieren, ohne die Gesamtstruktur zu verändern.

show abstract

Giant Artificial Ion Channels Formed by Self-Assembled, Cationic Rigid-Rodβ-Barrels

Cited by 12 publications

References 31 publications

Toward catalytic rigid‐rod β‐barrels: A hexamer with multiple histidines

Toward catalytic rigid‐rod β‐barrels: A hexamer with multiple histidines

Self-assembling subnanometer pores with unusual mass-transport properties

An Artificial Ion Channel Formed by a Macrocyclic Resorcin[4]arene with Amphiphilic Cholic Acid Ether Groups

Contact Info

Product

Resources

About