Giant linear plasmids of Î²-lactam antibiotic producing Streptomyces

Netolitzky, Donald; Wu, Xiaoning; Jensen, Susan E.; Roy, Kanak

doi:10.1111/j.1574-6968.1995.tb07749.x

Cited by 14 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strain of S. coelicolor (M145) from which the putative terminal DNA was cloned does not contain any free plasmid (Kieser et al ., 1992) and, therefore, was not tested. For S. clavuligerus , the 2.1 kb putative terminal fragment hybridized to a 120 kb linear plasmid, pSCL2 (Netolitzky et al ., 1995), and not to the chromosomal DNA (Fig. 3A).…”

Section: Resultsmentioning

confidence: 99%

“…In S. lividans , the chromosomal termini share a 15.3 kb homology with the right end of the linear plasmid SLP2 (Chen et al ., 1993; Lin et al ., 1993). S. clavuligerus harbours three linear plasmids (Netolitzky et al ., 1995), of which only the largest (pSCL3) exhibited extensive hybridization to the chromosomal DNA (Netolitzky et al ., 1995). In the other cases examined, no hybridization was detected between the terminal DNA of the linear plasmids and the host chromosome: those in S. griseus NRRL 3851 and S. jumonjinesis NRRL 5741 (Netolitzky et al ., 1995), SCP1 in S. coelicolor (H. Kinashi, personal communication) and pSPA1 in S. parvulus (Fig.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The telomeres of Streptomyces chromosomes contain conserved palindromic sequences with potential to form complex secondary structures

Huang¹,

Lin²,

Yang³

et al. 1998

Molecular Microbiology

108

119

View full text Add to dashboard Cite

SummaryThe chromosomes of the Gram-positive soil bacteria Streptomyces are linear DNA molecules, usually of about 8 Mb, containing a centrally located origin of replication and covalently bound terminal proteins (which are presumably involved in the completion of replication of the telomeres). The ends of the chromosomes contain inverted repeats of variable lengths. The terminal segments of five Streptomyces chromosomes and plasmids were cloned and sequenced. The sequences showed a high degree of conservation in the first 166-168 bp. Beyond the terminal homology, the sequences diverged and did not generally crosshybridize. The homologous regions contained seven palindromes with a few nucleotide differences. Many of these differences occur in complementary pairs, such that the palindromicity is preserved. Energyoptimized modelling predicted that the 3Ј strand of the terminal palindromes can form extensive hairpin structures that are similar to the 3Ј ends of autonomous parvovirus genomes. Most of the putative hairpins have a GCGCAGC sequence at the loop, with the potential to form a stable single C-residue loop closed by a sheared G:A pairing. The similarity between the terminal structures of the Streptomyces replicons and the autonomous parvoviral genomes suggests that they may share some structural and/or replication features.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The telomeres of Streptomyces chromosomes contain conserved palindromic sequences with potential to form complex secondary structures

Huang¹,

Lin²,

Yang³

et al. 1998

Molecular Microbiology

108

119

View full text Add to dashboard Cite

show abstract

“…Small linear plasmids were also found in S. azureus [3], S. rimosus [4] and S. clavuligerus [5]. Large linear plasmids include SCP1 (350 kb) in S. coelicolor A3(2) [1], pKSL (520 kb) in S. lasaliensis [1], pSA1 (100 kb) and pSA2 (250 kb) in S. avermitilis [6] and those in β‐lactam antibiotic‐producing Streptomyces [7]. Some of them were first identified genetically [8], before being physically detected by PFGE.…”

Section: Introductionmentioning

confidence: 99%

A linear plasmid temperature-sensitive for replication inStreptomyces hygroscopicus10-22

Pang¹,

Sun²,

Li³

et al. 2002

FEMS Microbiology Letters

View full text Add to dashboard Cite

Streptomyces hygroscopicus 10-22 harbors a conjugative, autonomously replicating linear plasmid pHZ6 of ca. 70 kb, which shows no obvious homology with chromosomal DNA and is temperature-sensitive for replication, being stable in the host at 28 degrees C but easily lost at 37 degrees C. On a lawn of the wild-type S. hygroscopicus 10-22 cured of pHZ6, pHZ6 elicit pocks. Temperature sensitivity seemed to be a unique property for pHZ6 among six linear plasmids tested, including the well-known linear plasmids SLP2 in Streptomyces lividans 1326 and SCP1 in Streptomyces coelicolor A3(2). The distinct identity of pHZ6 from previously identified pHZ1-pHZ5 was demonstrated by the profile of relevant plasmids in six well-defined strains originated from S. hygroscopicus 10-22.

show abstract

“…These plasmids can recombine with the chromosome, enhancing the genetic interchange of secondary metabolites (Medema et al, ). Interestingly, S. cattleya NRRL 3841 and S. lipmannii NRRL 3584 are two species of the genus lacking plasmids (Netolitzky, Wu, Jensen, & Roy, ), and even the strain S. clavuligerus F613‐1 has a different number of plasmids (Cao et al, ). These findings suggest that plasmids could provide a vehicle for genetic interchange by horizontal gene transfer or some other mechanism in this species and even in the genus.…”

Section: Introductionmentioning

confidence: 99%

A putative antimicrobial peptide from Hymenoptera in the megaplasmid pSCL4 of Streptomyces clavuligerus ATCC 27064 reveals a singular case of horizontal gene transfer with potential applications

Ayala-Ruano

Santander-Gordón

Tejera

et al. 2019

Ecology and Evolution

View full text Add to dashboard Cite

Streptomyces clavuligerus is a Gram‐positive bacterium that is a high producer of secondary metabolites with industrial applications. The production of antibiotics such as clavulanic acid or cephamycin has been extensively studied in this species; nevertheless, other aspects, such as evolution or ecology, have received less attention. Furthermore, genes that arise from ancient events of lateral transfer have been demonstrated to be implicated in important functions of host species. This approximation discovered relevant genes that genomic analyses overlooked. Thus, we studied the impact of horizontal gene transfer in the S. clavuligerus genome. To perform this task, we applied whole‐genome analysis to identify a laterally transferred sequence from different domains. The most relevant result was a putative antimicrobial peptide (AMP) with a clear origin in the Hymenoptera order of insects. Next, we determined that two copies of these genes were present in the megaplasmid pSCL4 but absent in the S. clavuligerus ATCC 27064 chromosome. Additionally, we found that these sequences were exclusive to the ATCC 27064 strain (and so were not present in any other bacteria) and we also verified the expression of the genes using RNAseq data. Next, we used several AMP predictors to validate the original annotation extracted from Hymenoptera sequences and explored the possibility that these proteins had post‐translational modifications using peptidase cleavage prediction. We suggest that Hymenoptera AMP‐like proteins of S. clavuligerus ATCC 27064 may be useful for both species adaptation and as an antimicrobial molecule with industrial applications.

show abstract

Giant linear plasmids of Î²-lactam antibiotic producing Streptomyces

Cited by 14 publications

References 23 publications

The telomeres of Streptomyces chromosomes contain conserved palindromic sequences with potential to form complex secondary structures

The telomeres of Streptomyces chromosomes contain conserved palindromic sequences with potential to form complex secondary structures

A linear plasmid temperature-sensitive for replication inStreptomyces hygroscopicus10-22

A putative antimicrobial peptide from Hymenoptera in the megaplasmid pSCL4 of Streptomyces clavuligerus ATCC 27064 reveals a singular case of horizontal gene transfer with potential applications

Contact Info

Product

Resources

About