Giant Magnetoresistive Based Handheld System for Rapid Detection of Human NT-proBNP

Abstract

In this work, we developed giant magnetoresistive (GMR) based handheld biosensing systems that serve as platform for detecting human NT-proBNP. This assay takes advantages of high sensitivity and real-time signal readout of GMR biosensor. The limit of detection was estimated to be less than 0.01ng/mL, and detection range covered from 0.01 ng/mL to 5 ng/mL was obtained. The assay can be completed within 20 min, which is very important for further development of point-of-care testing. The proposed GMR handheld system is also successfully used for the detection of real NT-proBNP human samples. It can be foreseen that this handheld detection system could become a robust contender in the applications of in vitro biomarker diagnostics.

Multifunctional Core@Satellite Magnetic Particles for Magnetoresistive Biosensors

“…It is worth pointing out that the GMR signal at a concentration in the order of 1 ng mL –1 is approximately 3 × 10 –3 , a value significantly larger than that obtained with a similar experimental layout but with “ordinary” magnetic beads . The relatively large GMR signal entailed by CSMPs is also comparable to that achieved by other groups who obtained approximately 500 ppm at 250 pg/mL for both human IL6 and Human NT-ProBNP . However, compared to our detection scheme (Figure ), the latter approach required an additional step since the MNPs tethered the secondary Abs rather than the analyte.…”

“… 32 The relatively large GMR signal entailed by CSMPs is also comparable to that achieved by other groups who obtained approximately 500 ppm at 250 pg/mL for both human IL6 33 and Human NT-ProBNP. 34 However, compared to our detection scheme ( Figure 1 ), the latter approach required an additional step since the MNPs tethered the secondary Abs rather than the analyte.…”