Gibberellic acid and thidiazuron promote micropropagation of an endangered woody tree (Pterocarpus marsupium Roxb.) using in vitro seedlings

Ahmad, Anees; Ahmad, Naseem; Anis, Mohammad; Alatar, Abdulrahman A.; Abdel-Salam, Eslam M.; Qahtan, Ahmed A.; Faisal, Mohammad

doi:10.1007/s11240-020-01969-1

Cited by 25 publications

(8 citation statements)

References 53 publications

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“…Root formation in in vitro derived micro-shoots is often problematic in woody species causing considerable economic effect at this stage (De Klerk 2002;Wiszniewska et al, 2016) and modified methods are often reported (Ahmad et al, 2021). The rooting percentage was low in the present study.…”

Section: Establishment Of Initial Cultures and Multiplication Phasecontrasting

confidence: 56%

“…Senna artemisioides mother plant, 7 years old, cultivated in a private botanical garden, at Peania (37°57'53.0"N 23°51'10.0"E, Attica, Greece), in September 2020 (A) blooming in May (B) fruiting in August (C) A simple, cost effective and quick protocol is crucial for achieving practical goals of exploitation of new species. The in vitro seedling method significantly reduces time taken to initiate in vitro cultures bypassing the explant preparation step (Ahmad et al, 2021). Starting from seeds is a widespread method to establish in vitro cultures of new ornamental species of high proliferation rate (Papafotiou et al, 2013;Cabahug et al, 2018).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

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In vitro germination and regeneration of Senna artemisioides, a valuable leguminous ornamental shrub

Bertsouklis

NAKSI

ARETAKI

2023

Not Bot Horti Agrobo

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Τhis research was carried out at the prospect of introducing new ornamental species in the floriculture industry concerning the use of valuable plants. A leguminous species native to Australia was the object of the present study. Seeds of Senna artemisioides established in a private botanical garden were chosen as the starting material for both germination and in vitro studies. Hot water treatment at 90 °C, for 10 min was applied to interrupt the seed-coat dormancy. Incubation was conducted in vitro in Petri dishes, at seven different temperatures ranging from of 5.0 to 35.0 °C, involving three separate treatments of incubation at 5.0 °C, for 0, 30 and 60 days. The seeds germinated at 25 °C (56.0%). Node explants derived from produced seedlings were cultured in vitro onto Murashige and Skoog media (MS), hormone free (Hf) or containing 6-Benzyladenine (BA). The establishment of initial cultures was successful being higher on MS supplemented with BA at the range 0.1-1.0 mg L-1 media. The multiplication phase followed on MS media containing the same range of a total of four cytokinins widely used in in vitro experimentation, i.e BA, Kinetin (KIN), N6-Isopentenyladenine (2IP) and Zeatin (ZEA). 1-Naphthaleneacetic acid (NAA) was tested in conjunction with the higher concentration of the four cytokinins. MS media supplemented with BA at 0.5 mg L-1 led to a satisfactory proliferation rate; 2.4 shoots/explant were formed. Rooting phase had moderate efficiency, being higher for media with 4.0 mg L-1 IBA. Acclimatisation was successful for all rooted plantlets, promising an effective method for exploitation of this new ornamental species of high value.

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Section: Establishment Of Initial Cultures and Multiplication Phasecontrasting

confidence: 56%

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

In vitro germination and regeneration of Senna artemisioides, a valuable leguminous ornamental shrub

Bertsouklis

NAKSI

ARETAKI

2023

Not Bot Horti Agrobo

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“…Half strength MS medium supplemented with 3.0 mg/l IBA possessed the highest frequency of root induction (95%) and number of roots per explant (10.2) as well as mean root length (2.6 cm). IBA induced root induction has been reported in other systems like Plumbago zeylanica (Sharma and Agarwal 2018), Pterocarpus marsupium (Ahmad et al 2021) and Swertia minor (Kshirsagar et al 2021) In conclusion, a robust shoot regeneration system through ower bud derived callus organogenesis has been standardized for G. febrifugum. This protocol could be utilized for the mass multiplication of this plant for commercial purpose.…”

Section: Rooting Of Shootsmentioning

confidence: 55%

High-frequency shoot regeneration from flower bud derived callus of Gymnostachyum febrifugum Benth., an endemic medicinal plant to the Western Ghats

Silpa

Thomas

2021

Plant Cell Tiss Organ Cult

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Gymnostachyum febrifugum Benth. is a small, scapigerous, rare and endemic medicinal herb indigenous to India belonging to the family Acanthaceae. This study reports an e cient protocol for high-frequency ower bud derived callus induction and shoot organogenesis in G. febrifugum. Flower buds at 7d before anthesis (dBA) were excised from the in orescence and cultured on MS medium supplemented with various concentrations of 2, 4-dichlorophenoxy acetic acid (2, 4-D; 0.5-2.0 mg/l) for callus induction. The optimum callus induction (78%) was obtained on MS medium supplemented with 1.5 mg/l 2, 4-D. The calli when subcultured on MS medium supplemented with different concentrations of thidiazuron (TDZ; 0.5-2.5 mg/l) or 6-benzylaminopurine BAP (0.5-2.5 mg/l) alone or in combination with 1naphthaleneacetic acid (NAA; 0.2-0.7 mg/l) induced shoots. The highest frequency (94%) and number of shoots (44.6 shoots/unit callus) were obtained on MS medium supplemented with 2.0 mg/l TDZ and 0.5 mg/l NAA. The optimum rooting frequency (95%) and number of roots (10.2) were observed on ½ MS medium supplemented with 3.0 mg/l indole-3-butyric acid (IBA). The rooted plantlets were acclimatized and transferred to soil with 94% success.

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“…The combination of GA 3 and TDZ in the culture media of Pterocarpus marsupium Roxb. improved the germination percentage, multiplication, and subsequent elongation of shoots [38]. The use of 1 and 5 mg•L −1 GA 3 resulted in 80.5 and 92.3% root induction in Cynara scolymus L. [36].…”

Section: Discussionmentioning

confidence: 94%

In Vitro Shoot Multiplication and Rooting of ‘Kashan’ and ‘Hervy Azerbaijan’ Damask Rose (Rosa damascena Mill.) Genotypes for Cosmetic and Ornamental Applications

Kaviani,

Deltalab,

Kulus

et al. 2024

Plants

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The damask rose (Rosa damascena Mill.) is an ornamental–medicinal plant from the Rosaceae family, and its aromatic compounds and essential oils are applied globally in the food, cosmetic, and pharmaceutical industries. Due to its economic value, this research aimed to establish a protocol for an efficient, rapid, and cost-effective method for in vitro shoot multiplication and rooting of the R. damascena ‘Kashan’ and ‘Hervy Azerbaijan’ genotypes. Nodal segments (as primary explants) were cultured on the Murashige and Skoog (MS) medium with combinations of various plant growth regulators (PGRs) such as gibberellic acid (GA3), 6-benzylaminopurine (BAP), and indole-3-butyric acid (IBA), as well as a PGR-like substance, phloroglucinol (PG), vitamins such as ascorbic acid (AA), and activated carbon in the form of active charcoal (AC). For the establishment stage, 0.1 mg·L−1 PG, 0.2 mg·L−1 GA3, and 1 mg·L−1 BAP were added to the media. Secondary explants (nodal segments containing axillary buds produced from primary explants) were obtained after 30 days of in vitro culture and transferred to the proliferation media supplemented with different concentrations of BAP (0, 0.5, 1, 1.5, 2, and 2.5 mg·L−1) and GA3 (0, 0.1, 0.2, 0.4, 0.8, and 1 mg·L−1) together with 0.1 mg·L−1 PG and 20 mg·L−1 of AA. The rooting media were augmented with different concentrations of BAP and GA3 with 0.1 mg·L−1 of IBA, PG and 20 mg·L−1 of AA and AC. The results showed that the highest regeneration coefficient (4.29 and 4.28) and the largest number of leaves (23.33–24.33) were obtained in the explants grown on the medium supplemented with 2 mg·L−1 BAP and 0.4 mg·L−1 GA3 for the ‘Kashan’ and ‘Hervy Azerbaijan’ genotypes, respectively. Likewise, this PGR combination provided the shortest time until bud break (approximately 6.5 days) and root emergence (approximately 10 days) in both genotypes. The highest number of shoots (4.78 per explant) and roots (3.96) was achieved in this medium in the ‘Kashan’ rose. Stem and root lengths, as well as stem and root fresh and dry weights, were also analyzed. In most measured traits, the lowest values were found in the PGRs-free control medium. Rooted plantlets were transferred to pots filled with perlite and peat moss in a 2:1 proportion and were acclimatized to ambient greenhouse conditions with a mean 90.12% survival rate. This research contributes significantly to our understanding of Damask rose propagation and has practical implications for the cosmetic and ornamental plant industries. By offering insights into the manipulation of regeneration processes, our study opens up new possibilities for the effective production of high-quality plant material.

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Gibberellic acid and thidiazuron promote micropropagation of an endangered woody tree (Pterocarpus marsupium Roxb.) using in vitro seedlings

Cited by 25 publications

References 53 publications

In vitro germination and regeneration of Senna artemisioides, a valuable leguminous ornamental shrub

In vitro germination and regeneration of Senna artemisioides, a valuable leguminous ornamental shrub

High-frequency shoot regeneration from flower bud derived callus of Gymnostachyum febrifugum Benth., an endemic medicinal plant to the Western Ghats

In Vitro Shoot Multiplication and Rooting of ‘Kashan’ and ‘Hervy Azerbaijan’ Damask Rose (Rosa damascena Mill.) Genotypes for Cosmetic and Ornamental Applications

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