Gibberellic acid-mediated male sterility during gametogenesis of Brassica campestris L.

Abstract

The present work was done to elucidate the impact of gibberellic acid (GA 3) on male meiosis of Brassica campestris L. with an aim to study its cytological peculiarities. Microsporogenesis analysis of distilled water treated sets displayed the normal meiotic and post-meiotic courses. However GA 3 sprayed sets possessed several meiotic manifestations followed by disturbed postmeiosis. In present case meiotic irregularities followed by unusual tetrad configurations such as tetrad having randomly arranged microspores, completely vacant (sterile) tetrad, monad (microsporesac bearing one microspore), dyad (two microspores), triad (three microspores) and polyads (more than four microspores) were registered. As a consequence of such an anomalous microsporogenesis, pollen fertility rate of the present crop has been declined considerably in a dose dependent manner whereas its direct and negative influence on the reproductive success of the test crop has been determined. In ours study, marked dropdown in the reproductive success has been recorded; which was estimated on the basis of rate of increased pollen abortion which in turn led to considerable decreased seed set of crop at maturity.

Automated monitoring of pollen quality in Douglas fir using digital images: applications for handling and storage

“…It is an actively pursued practice within genetic improvement programs, and for enhancing seed production in plantations. Hence, monitoring the quantity and quality of pollen is essential to ensure good fertilization and seed production (Ho 1991, Kears & Inouye 1993, Chhun et al 2007, Kumar & Dwivedi 2014.…”

“…B.S.P. (Ho 1991), as well as in angiosperms such as Oryza sativa (Chhun et al 2007) and Brassica campestris L. (Kumar & Dwivedi 2014).…”