Gibberellic Acid Regulates the Level of a BiP Cognate in the Endoplasmic Reticulum of Barley Aleurone Cells

Jones, Russell L.; Bush, Douglas

doi:10.1104/pp.97.1.456

Cited by 30 publications

(15 citation statements)

References 11 publications

(31 reference statements)

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“…Some of these Ca; +-binding proteins, such as the soluble ER protein BiP, have been shown in yeast to be essential for proper processing of secreted proteins. Using antibodies to a yeast BiP, we have identified a BiP cognate in the ER of barley aleurone whose levels are increased by GA3 and decreased by ABA (Jones and Bush 1991). The 45Ca2+-overlays we present here indicate that, in addition to BiP, there are several Ca2+-binding proteins in the ER whose levels are increased by GA3.…”

Section: Discussionmentioning

confidence: 69%

Hormonal regulation of Ca2+ transport in the endomembrane system of the barley aleurone

Bush

Biswas

Jones

1993

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Abstract. Gibberellic acid (GA3) and abscisic acid (ABA)regulate a-amylase production in aleurone tissue of the barley (Hordeum vulgare L.) grain during germination. Since s-amylase is a Ca2+-containing protein that is synthesized on the rough endoplasmic reticulum (ER), we investigated the possibility that GA 3 and ABA regulate the flux of Ca 2+ into the ER of aleurone cells. Membrane vesicles were isolated from GA 3-and ABA-treated aleurone layers to characterize the effect of these hormones on calcium transport (CAT) activity as measured by the uptake of 45CAC12 in vitro. We found that ABA and GA 3 greatly alter the relative amounts of CaT activity in ER and tonoplast (TP) membrane fractions. Treatment with GA 3 results in CaT being associated primarily with the ER, whereas treatment with ABA results in roughly equal amounts of CaT in ER and TP. Moreover, we found that ABA prevents or reverses the GA3-induced increase in CaT in the ER. The effects of ABA and GA3 on CaT in the ER require several hours to develop, and the effects of these hormones on CaT cannot be brought about by adding the hormones to membranes in vitro. In order to determine whether the in-vitro measurements of CaT reflect changes that occur in vivo we measured the Ca 2+ content of isolated ER, measured Ca 2 + transport into aleurone layers, and examined differences in the ability of ER proteins from GA-and ABAtreated layers to bind Ca 2+. Our results indicate that GA 3 increases and ABA decreases both Ca 2+ flux into the ER and the amount of calcium that accumulates in the ER of barley aleurone cells in vivo.

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Section: Discussionmentioning

confidence: 69%

Hormonal regulation of Ca2+ transport in the endomembrane system of the barley aleurone

Bush

Biswas

Jones

1993

Planta

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“…In aleurone, a 16-h treatment with GA3 stimulated the synthesis of BiP (Jones and Bush 1991). Synthesis of maize BiP was not stimulated by GA3 or ABA during the 3-h labelling period (Fig.…”

Section: Discussionmentioning

confidence: 85%

Regulation of synthesis and turnover of maize auxin-binding protein and observations on its passage to the plasma membrane: comparisons to maize immunoglobulin-binding protein cognate

Oliver¹,

Venis²,

Freedman

et al. 1995

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Electrophysiological experiments have indicated that a fraction of the major auxin-binding protein (ABP1) of maize (Zea mays L.) might be a receptor on the outer surface of the plasma membrane. The predominant location of ABP1 is in the lumen of the endoplasmic reticulum (ER), in accord with its C-terminal KDEL retention signal. Little is known about the biology of the protein in vivo or the rate at which it might pass to the cell surface. We have examined the turnover of ABP1 by in vivo labelling of maize coleoptile sections. After different chase times, ABP1 was immunoprecipitated from detergent-solubilised membrane preparations. Two polypeptides coprecipitated with ABP1. Neither was recognised by any ABP1 antibodies nor by monoclonals to ER retention sequences. The possible significance of these coprecipitating polypeptides is discussed. In addition, we have used a monoclonal antibody to precipitate HDEL proteins from the same membrane preparations. Two dimensional electrophoresis and N-terminal sequencing showed that the major HDEL protein precipitated was a member of the heat-shock-protein 70 family, a homologue of BiP (immunoglobulin-binding protein). We have investigated the turnover of this BiP homologue for comparison with ABP1 and found that both had extended lifetimes, with half-lives greater than 24 h. Use of cordycepin to inhibit transcription indicated that ABP1 mRNA was also long-lived. Synthesis of ABP1 was strongly reduced by heat stress, was reduced a little in response to dithiothreitol and was not markedly changed by tunicamycin. In contrast, BiP synthesis increased markedly in response to tunicamycin and dithiothreitol and increased a little after heat stress.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…During germination of barley seeds, the embryo synthesizes gibberellins, which trigger the production of secreted hydrolases on the RER in aleurone cells (Jones, 1985). Gibberellin also induces BiP levels (Jones and Bush, 1991) before gibberellin-induced hydrolase production . This response thus may be similar to the rapid induction of BiP during plant-pathogen interactions in anticipation of increased protein synthesis on the RER.…”

Section: Bip Induction In Anticipation Of Er Stress: a Novel Process mentioning

confidence: 97%

Anticipating Endoplasmic Reticulum Stress: A Novel Early Response before Pathogenesis-Related Gene Induction

1999

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When it is attacked by a pathogen, a plant produces a range of defense-related proteins. Many of these are synthesized by the rough endoplasmic reticulum (RER) to be secreted from the cell or deposited in vacuoles. Genes encoding endoplasmic reticulum (ER)-resident chaperones, such as the lumenal binding protein (BiP), are also induced under these conditions. Here, we show that BiP induction occurs systemically throughout the plant. Furthermore, this induction occurs rapidly and precedes expression of genes encoding pathogenesis-related (PR) proteins. The underlying signal transduction pathway was shown to be independent of the signaling molecule salicylic acid and the unfolded protein response pathway. In addition, BiP induction was independent of PR gene induction. Overproduction of BiP alone was not sufficient to cause induction of PR gene expression; however, limiting the amount of BiP in the ER lumen via superimposed ER stress inhibited the induction of PR gene expression. We propose that the induction of BiP expression during plant-pathogen interactions is required as an early response to support PR protein synthesis on the RER and that a novel signal transduction pathway exists to trigger this rapid response.

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Gibberellic Acid Regulates the Level of a BiP Cognate in the Endoplasmic Reticulum of Barley Aleurone Cells

Cited by 30 publications

References 11 publications

Hormonal regulation of Ca2+ transport in the endomembrane system of the barley aleurone

Hormonal regulation of Ca2+ transport in the endomembrane system of the barley aleurone

Regulation of synthesis and turnover of maize auxin-binding protein and observations on its passage to the plasma membrane: comparisons to maize immunoglobulin-binding protein cognate

Anticipating Endoplasmic Reticulum Stress: A Novel Early Response before Pathogenesis-Related Gene Induction

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