Gibberellin-induced changes in the transcriptome of grapevine (Vitis labrusca × V. vinifera) cv. Kyoho flowers

Cheng, Chenxia; Chen, Jiao; Singer, Stacy D.; Gao, Min; Xu, Xiaozhao; Zhou, Yiming; Li, Zhi; Fei, Zhangjun; Wang, Yuejin; Wang, Xiping

doi:10.1186/s12864-015-1324-8

Cited by 83 publications

(62 citation statements)

References 71 publications

(76 reference statements)

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“…However, to our knowledge, there is no information available regarding the length of the juvenile period in K. longiflora. Similar to our results, GA 3 has been reported to reduce the juvenile period of Tagetes erecta (Kumar et al, 2014) and stimulate the rate of flower development of Vitis labrusca • Vitis vinifera cultivar Kyoho (Cheng et al, 2015).…”

Section: Growth Regulators Discussionsupporting

confidence: 92%

“…The results of the study show the potential to accelerate flowering in Kalancho€ e, as observed in several other ornamental species (Cardoso et al, 2012;Chen et al, 2003;Cheng et al, 2015;Kumar et al, 2014;Sarkar et al, 2014;Wadhi and Ram, 1967). However, the proposed methodology can be suitable for flowering induction in the breeding programs.…”

Section: Growth Regulators Discussionmentioning

confidence: 61%

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Exogenous Application of Gibberellic Acid Improves Flowering in Kalanchoë

Coelho¹,

Fkiara²,

Mackenzie³

et al. 2018

horts

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Kalanchoë is an economically important genus comprising numerous potted plants and recently is also emerging as cut flowers. However, the lack of information about flower-inducing factors limits the number of species that can be used in commercial production and breeding programs. Therefore, the aim of this study was to investigate the effects of exogenous application of gibberellic acid (GA3) on flower induction and flowering quality of Kalanchoë longiflora and Kalanchoë pinnata. The experiment was conducted under a short day (SD) photoperiod with a day temperature of 22 °C and a night temperature of 15 °C for 8 weeks. The treatments consisted of four applications of either 0.25 or 0.50 μg of GA3 per plant per week, providing a total of 100 μg or 200 μg/plant and 0 μg/plant for the control. The volume of 100 μL of GA3 solution containing 1% agarose was applied to the shoot apex using a pipette. For both species, flowering was enhanced by the GA3 treatments compared with the control plants. Gibberellin-treated plants flowered earlier, produced more inflorescences, and exhibited an increased number of flowers compared with the control plants. Moreover, the GA3 treatments in K. longiflora delayed the appearance of wilted flowers. Plant height increased in plants that received GA3, but the number of nodes did not differ from the control plants. Thus, we conclude that the application of GA3 improves flowering of Kalanchoë species and can be a useful tool for the production of cut flower cultivars.

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Section: Growth Regulators Discussionsupporting

confidence: 92%

Section: Growth Regulators Discussionmentioning

confidence: 61%

Exogenous Application of Gibberellic Acid Improves Flowering in Kalanchoë

Coelho¹,

Fkiara²,

Mackenzie³

et al. 2018

horts

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show abstract

“…The genes of GA2ox which encode the GA-inactivating enzymes exhibited differential expression after the application of GA3, Glyma.18G061300 (GA2ox 1) was up-regulated by log2 FC of 1.93. These changes coincided with the feedback mechanism of the responsiveness of tissue to the bioactive GAs influencing GA metabolism (Cheng et al 2015). Glyma.13G259500 (GA2ox 8) which hydroxylates the C20-GA GA12 and GA53 (Schomburg et al 2003), was down-regulated by log2 FC -3.61.…”

Section: Resultsmentioning

confidence: 62%

Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networks

Han¹,

Shi²,

Gao³

et al. 2020

Biol. plant.

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Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood. In this study, GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area were significantly inhibited in early stages after GA3 treatment. Microscopic examination showed that GA3 treatment changed the cortex thickness, the pericycle diameter, and cell size in main root. Interestingly, exogenous GA3 increased the quantity of lateral root primordia (LRP), but LR number decreased in this period. Moreover, the content of GAs, auxin and abscisic acid in root was altered. RNA-seq results revealed that application of GA3 not only changed the expression of genes in GA biosynthesis pathway, including GA20ox and GA2ox, but also the GA regulation genes and signalling pathway genes. The changes in expression of gene concerning other hormones were also detected. In addition, GA3 altered cell wall biogenesis and degradation genes which might be related to the changes of root morphology. In response to increased GA3, 103 transcription factors were detected. Thus, exogenous GA3 changed the content of hormones in roots and affected the root development by regulating the expression of respective genes.

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“…DELLA proteins are transcriptional repressors and downregulate GA response genes. In the presence of gibberellin, the stable GID1-GA-DELLA complex is recognized by the SCF SLY1 complex which ubiquintylates the DELLA proteins and causes their degradation by the 26S proteasome [71,72]. It has been reported previously that GID1-transcripts are upregulated during bud dormancy in grapevine while transcripts of DELLA are downregulated [73].…”

Section: Discussionmentioning

confidence: 99%

Characterization of genes and alleles involved in the control of flowering time in grapevine

Kamal

Ochßner

Schwandner

et al. 2019

Preprint

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34 Grapevine (Vitis vinifera) is one of the most important perennial crop plants in worldwide. 35 Understanding of developmental processes like flowering, which impact quality and quantity of yield 36 in this species is therefore of high interest. This gets even more important when considering some of 37 the expected consequences of climate change. Earlier bud burst and flowering, for example, may 38 result in yield loss due to spring frost. Berry ripening under higher temperatures will impact wine 39 quality. Knowledge of interactions between a genotype or allele combination and the environment 40 can be used for the breeding of genotypes that are better adapted to new climatic conditions. To this 41 end, we have generated a list of more than 500 candidate genes that may play a role in the timing of 42 flowering. The grapevine genome was exploited for flowering time control gene homologs on the 43 basis of functional data from model organisms like A. thaliana. In a previous study, a mapping 44 population derived from early flowering GF.GA-47-42 and late flowering 'Villard Blanc' was 45 analyzed for flowering time QTLs. In a second step we have now established a workflow combining 46 amplicon sequencing and bioinformatics to follow alleles of selected candidate genes in the F 1 47 individuals and the parental genotypes. Allele combinations of these genes in individuals of the 48 mapping population were correlated with early or late flowering phenotypes. Specific allele 49 combinations of flowering time candidate genes within and outside of the QTL regions for flowering 50 time on chromosome 1, 4, 14, 17, and 18 were found to be associated with an early flowering 51 phenotype. In addition, expression of many of the flowering candidate genes was analyzed over 52 consecutive stages of bud and inflorescence development indicating functional roles of these genes in 53 the flowering control network. 54 Introduction 55 The reproductive developmental cycle of grapevine spans two years (S1 Figure). Grapevine plants 56 need intense light and high temperatures to initiate inflorescences during spring, which develop and 57 flower during the subsequent summer [1]. The ongoing tendency to higher temperatures in spring 58 due to global warming causes earlier bud burst and flowering [2]. As a consequence, late spring 59 frost is an increasing risk to viticulture, which may cause significant crop loss [3]. Together with 60 flowering the onset of ripening is shifted towards earlier dates [4,5] and the ripening process occurs 61 under warmer conditions. This influences berry composition [6], affects wine quality and promotes 62 e.g. fungi infection. Grapevine breeding programs aim to keep the production of high quality grapes 63 in a changing environment consistent. Making use of late flowering genotypes may be one approach 64 to compensate for earlier ripening. Understanding the flowering process in grapevine and 65 determining factors that lead to early or late flowering may help to control variation in berry 66 production [7]....

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Gibberellin-induced changes in the transcriptome of grapevine (Vitis labrusca × V. vinifera) cv. Kyoho flowers

Cited by 83 publications

References 71 publications

Exogenous Application of Gibberellic Acid Improves Flowering in Kalanchoë

Exogenous Application of Gibberellic Acid Improves Flowering in Kalanchoë

Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networks

Characterization of genes and alleles involved in the control of flowering time in grapevine

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