Gibberellin-induced mesocotyl elongation in deep-sowing tolerant maize inbred line 3681-4

Abstract

In order to elucidate the molecular mechanism of gibberellin (GA)-induced mesocotyl elongation, gene expression profiling analyses were performed in a deep-sowing tolerant maize inbred line 3681-4. Gene expression studies combining Affymetrix GeneChip analysis and Realtime PCR were employed to determine the molecular mechanism underlying GA promotion of maize mesocotyl elongation. These studies showed that a mount of genes, such as the GA receptor GID1, the transcriptional factor MYB, and the genes encoding the DELLA protein DWRF8, kinases, Raf, LRR and RLCK, might play a critical role in maize mesocotyl elongation. Moreover, the genes that were involved in flavonoid biosynthesis, aminosugars metabolism, cell wall synthesis and modification were also found to be responsible for this process.

Gene cloning of ZmMYB59 transcription factor in maize and its expression during seed germination in response to deep‐sowing and exogenous hormones

“…However, its expression was upregulated as germination time went on at 0 cm sowing depth. The results suggest the key factor for seed germination of maize in deep soil (Troyer, 1997;Zhao et al, 2010). However, there were no obvious laws of ZmMYB59 expression in plumules or roots.…”

“…The results coincide with the trends of ZmMYB59 expression under the conditions of deep‐sowing. As is well known, mesocotyl elongation is the key factor for seed germination of maize in deep soil (Troyer, ; Zhao et al., ). However, there were no obvious laws of ZmMYB59 expression in plumules or roots.…”

“…In maize, there are about 200 MYB members, but the number of known MYB genes is limited. These MYB genes are mainly involved in the regulation of phenylpropanoid metabolism, anthocyanin synthesis, lignin formation, cell differentiation, secondary wall synthesis, leaf primordium development, formation of heterosis, stress response and so on (Cone et al, 1993;Du, Feng, Yang, Huang, & Tang, 2012;Fornal e et al, 2009;Franken, Schrell, Peterson, Saedler, & Wienand, 1994;Ju et al, 2006;Paz-Ares, Ghosal, Wienand, Peterson, & Saedler, 1987;Piazza, Procissi, Jenkins, & Tonelli, 2002;Timmermans, Hudson, Becraft, & Nelson, 1999;Zhang et al, 2016;Zhao et al, 2010;Zhong et al, 2011).…”

Expression of MYB transcription factor gene ZmMYB59 affects seed germination in Nicotiana tabacum and Oryza sativa

“…Nonetheless, the contributions of MYB transcription factors during seed germination have not yet been functionally characterized. In our previous study, to elucidate the molecular mechanisms of maize seed germination at 20 cm sowing depth, gene expression of mesocotyl was analyzed by Affymetrix GeneChip [15]. The results demonstrated that there was the highest expression level of MYB genes, accounting for 8.15% of total number of transcription factor family [15].…”

“…In our previous study, a new MYB transcription factor gene named ZmMYB59, was cloned from the B73 inbred line. Affymetrix GeneChip was employed to analyze the gene expression patterns of mesocotyl tissue during maize seed germination at 20 cm sowing depth, indicating that MYB genes were significantly expressed [15]. Thereafter, Real-Time PCR showed the expression of ZmMYB59 in maize mesocotyl was fairly down-regulated during seed germination [16].…”