Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers

Bai, Wenqin; Xiao, Yazhong; Jun, Zhao; Song, Shuiqing; Hu, Lin; Zeng, Jianyan; Li, Xianbi; Hou, Lei; Luo, Ming; Li, De-Mou; Pei, Yan

doi:10.1371/journal.pone.0096537

Cited by 64 publications

(46 citation statements)

References 38 publications

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“…For example, in G. arboreum there were several light-related motifs, like MCACGTGGC (G box, S000041), and stem internode response motifs, like TAGTGGAT (NRRBNEXTA, S000242), which may modulate stem development. In G. hirsutum , cis-elements, like the sugar repression-related motif TACGTA (A-box, S000130) and the gibberellin response motif GATGAYRTGG(OPAQUE2ZMB32, S000077) (48), occurred more often and we hypothesize these modulate cotton fiber development.…”

Section: Functional Applicationsmentioning

confidence: 98%

ccNET: Database of co-expression networks with functional modules for diploid and polyploidGossypium

You

Zhang

et al. 2016

Nucleic Acids Res

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Plant genera with both diploid and polyploid species are a common evolutionary occurrence. Polyploids, especially allopolyploids such as cotton and wheat, are a great model system for heterosis research. Here, we have integrated genome sequences and transcriptome data of Gossypium species to construct co-expression networks and identified functional modules from different cotton species, including 1155 and 1884 modules in G. arboreum and G. hirsutum, respectively. We overlayed the gene expression results onto the co-expression network. We further provided network comparison analysis for orthologous genes across the diploid and allotetraploid Gossypium. We also constructed miRNA-target networks and predicted PPI networks for both cotton species. Furthermore, we integrated in-house ChIP-seq data of histone modification (H3K4me3) together with cis-element analysis and gene sets enrichment analysis tools for studying possible gene regulatory mechanism in Gossypium species. Finally, we have constructed an online ccNET database (http://structuralbiology.cau.edu.cn/gossypium) for comparative gene functional analyses at a multi-dimensional network and epigenomic level across diploid and polyploid Gossypium species. The ccNET database will be beneficial for community to yield novel insights into gene/module functions during cotton development and stress response, and might be useful for studying conservation and diversity in other polyploid plants, such as T. aestivum and Brassica napus.

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Section: Functional Applicationsmentioning

confidence: 98%

ccNET: Database of co-expression networks with functional modules for diploid and polyploidGossypium

You

Zhang

et al. 2016

Nucleic Acids Res

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“…Giving an insight into previously reported specificity of GhSCFP [15,34,35], the expression pattern of CpEXPA1 under GhSCFP promoter was found to be higher in Cotton boll than that of the CaMV35S. The GhSCFP, isolated from Cotton, when allowed to express in Arabidopsis,…”

Section: Plos Onementioning

confidence: 80%

“…The in-silico joining of NCBI retrieved Expansin-like A1 gene sequence with two different promoters was done i.e. constitutive Cauliflower Mosaic Virus, CamV35S, [14] and Gossypium hirsutum seed coat Fiber-specific protease promoter, GhSCFSP, [15] with final gene constructs as 35SEXPA1 and FSEXPA1 respectively (Fig 1A and 1B). Protein translation, Codon optimization, functional protein synthesis, and restriction site analysis were done in silico through online tools of Expert Protein Analysis System (ExPASy) [16], Integrated DNA Technology website [17], ExPASy and WEB-CUTTER 2.0 [18] respectively.…”

Section: Plasmid Construction and Cloningmentioning

confidence: 99%

Comparative analysis of Constitutive and fiber-specific promoters under the expression pattern of Expansin gene in transgenic Cotton

et al. 2020

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Promoters are specified segments of DNA that lead to the initiation of transcription of a specific gene. The designing of a gene cassette for plant transformation is significantly dependent upon the specificity of a promoter. Constitutive Cauliflower mosaic virus promoter, CaMV35S, due to its developmental role, is the most commonly used promoter in plant transformation. While Gossypium hirsutum (Gh) being fiber-specific promoter (GhSCFP) specifically activates transcription in seed coat and fiber associated genes. The Expansin genes are renowned for their versatile roles in plant growth. The overexpression of Expansin genes has been reported to enhance fiber length and fineness. Thus, in this study, a local Cotton variety was transformed with Expansin (CpEXPA1) gene, in the form of two separate cassettes, each with a different promoter, named as 35SEXPA1 and FSEXPA1 expressed under CaMV35S and GhSCFP promoters respectively. Integration and Spatiotemporal relative expression of the transgene were studied in an advanced generation. GhSCFP bearing transgene expression was significantly higher in Cotton fiber than other plant parts. While transgene with CaMV35S promoter was found to be continually expressing in all tissues but the expression was lower in fiber than that expressed under GhSCFP. The temporal expression profile was quite interesting with a gradual increasing pattern of both constructs from 1DPA (days post anthesis) to 18DPA and decreased expression from 24 to 30 DPA. Besides the relative expression of promoters, fiber cellulose quantification and fluorescence intensity were also observed. The study significantly compared the two most commonly used promoters and it is deduced from the results that the GhSCFP promoter could be used more efficiently in fiber when compared with CaMV35S which being constitutive in nature preferred for expression in all parts of the plant.

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“…Currently, cotton fibre has become a trait of primary interest and several efforts have been made (Table 1) to identify fibrerelated genes and their functions to improve fibre quality such as E6 (John and Crow, 1992), GhExp1 (Harmer et al, 2002), GhSusA1 (Jiang et al, 2012), PIP2s (Li et al, 2013b) and GA20ox (Bai et al, 2014). Additionally, actin cytoskeleton (Li et al, 2005), polysaccharide biosynthesis, signal transduction and protein translocation (Sun et al, 2017)-related genes are also preferentially expressed in various fibre developmental pathways.…”

Section: Fibre Qualitymentioning

confidence: 99%

Recent insights into cotton functional genomics: progress and future perspectives

Ashraf

Zuo

Wang

et al. 2018

Plant Biotechnology Journal

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SummaryFunctional genomics has transformed from futuristic concept to well‐established scientific discipline during the last decade. Cotton functional genomics promise to enhance the understanding of fundamental plant biology to systematically exploit genetic resources for the improvement of cotton fibre quality and yield, as well as utilization of genetic information for germplasm improvement. However, determining the cotton gene functions is a much more challenging task, which has not progressed at a rapid pace. This article presents a comprehensive overview of the recent tools and resources available with the major advances in cotton functional genomics to develop elite cotton genotypes. This effort ultimately helps to filter a subset of genes that can be used to assemble a final list of candidate genes that could be employed in future novel cotton breeding programme. We argue that next stage of cotton functional genomics requires the draft genomes refinement, re‐sequencing broad diversity panels with the development of high‐throughput functional genomics tools and integrating multidisciplinary approaches in upcoming cotton improvement programmes.

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Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers

Cited by 64 publications

References 38 publications

ccNET: Database of co-expression networks with functional modules for diploid and polyploidGossypium

ccNET: Database of co-expression networks with functional modules for diploid and polyploidGossypium

Comparative analysis of Constitutive and fiber-specific promoters under the expression pattern of Expansin gene in transgenic Cotton

Recent insights into cotton functional genomics: progress and future perspectives

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