Gibberellin oxidase activities in Bradyrhizobium japonicum bacteroids

Méndez, Constanza; Baginsky, Cecilia; Hedden, Peter; Gong, Fan; Carú, Margarita; Rojas, María Cecilia

doi:10.1016/j.phytochem.2013.11.013

Cited by 30 publications

(45 citation statements)

References 37 publications

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Section: The Role Of Plant Hormones In Nodulationmentioning

confidence: 58%

“…Given that this work did not demonstrate a production or even presence of GA3, as was found in the liquid culture of B. japonicum by Boiero et al (2007), it is likely that the biosynthesis pathway in bacteroids differs to that of the free-living bacterium (Méndez et al, 2014). This is possibly due to the low O2 environment of the nodule interior which induces specific GA biosynthesis enzymes (Méndez et al, 2014). It has been suggested that bacteroids do not produce bioactive GAs directly, instead they secret GA9 into the plant tissue, possibly to then be converted via the plant biosynthesis pathway into bioactive GAs (Méndez et al, 2014).…”

Section: The Role Of Plant Hormones In Nodulationmentioning

confidence: 62%

“…Recent work by Méndez et al (2014) has enabled the proposal of the GA biosynthesis pathway present in B. japonicum bacteroids. Given that this work did not demonstrate a production or even presence of GA3, as was found in the liquid culture of B. japonicum by Boiero et al (2007), it is likely that the biosynthesis pathway in bacteroids differs to that of the free-living bacterium (Méndez et al, 2014).…”

Section: The Role Of Plant Hormones In Nodulationmentioning

confidence: 99%

“…This is possibly due to the low O2 environment of the nodule interior which induces specific GA biosynthesis enzymes (Méndez et al, 2014). It has been suggested that bacteroids do not produce bioactive GAs directly, instead they secret GA9 into the plant tissue, possibly to then be converted via the plant biosynthesis pathway into bioactive GAs (Méndez et al, 2014).…”

Section: The Role Of Plant Hormones In Nodulationmentioning

confidence: 99%

“…then also converted to GA9 17-nor-16-one (Figure 1-1; Méndez et al, 2014). The exact components which act in the bacteroid biosynthesis pathway to convert GAs from one to another are not known; this presents another possible difference in the bacteroid pathway (Méndez et al, 2014).…”

Section: The Role Of Plant Hormones In Nodulationmentioning

confidence: 99%

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Molecular genetic and biochemical analysis of gibberellic acid involvement in the stages of soybean (Glycine max L.) nodule development

Potten¹

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This thesis aimed to characterise four genes identified to be involved in early nodulation in Glycine max (soybean). The first gene studied was a GmTIR-NBS-LRR gene, a Toll/Interleukin 1 receptor -Nucleotide Binding Site -Leucine-Rich Repeat (TIR-NBS-LRR) suspected of being involved in host specificity in regards to rhizobia. Over-expression, silencing and histochemical promoter studies of this gene saw no phenotypic changes. This is due to the previously unknown existence of a transcript variant (TV) of the gene as well as the likely existence of alternative promoter(s) (AP). Future characterisation work will focus on understanding and identifying the TVs and possible APs of GmTIR-NBS-LRR.The remaining three early nodulation genes are involved in GA biosynthesis, a plant hormone whose role in nodulation is still unclear. The genes GmGA20ox a, GmGA2ox and GmGA3ox 1a were analysed through silencing and histochemical promoter studies, over-expression and phytogenic analysis and histochemical promoter studies, respectably. Additionally, measurement of endogenous GAs in G. max roots which had yet to be reported was carried out.No phenotypic changes were observed following either silencing or over-expression for any of the GA biosynthesis genes. The histochemical promoter studies of GmGA20ox a highlighted likely role in facilitating infection thread formation and early nodule development in the cortical cells. GmGA3ox 1a appeared to be more general, but still NF dependent in its expression, through its widespread presence in the phloem during early nodulation.Successful measurement of endogenous GA has shown that independent of nodulation, GA3 is the more abundant bioactive GA in soybean compared to GA1. This differs from Pisum sativum (pea) roots where GA3 was not detected and GA1 is most abundant.The recent availability of G. max mutants of many of these genes through Soybase (http://soybase.org), coupled with the new method for measuring endogenous GA in G. max roots, opens up many pathways for further effective characterisation of these nodulation specific genes and thus, a better understanding of nodulation as a whole.

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Section: The Role Of Plant Hormones In Nodulationmentioning

confidence: 58%

Section: The Role Of Plant Hormones In Nodulationmentioning

confidence: 62%

Section: The Role Of Plant Hormones In Nodulationmentioning

confidence: 99%

Section: The Role Of Plant Hormones In Nodulationmentioning

confidence: 99%

Section: The Role Of Plant Hormones In Nodulationmentioning

confidence: 99%

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Molecular genetic and biochemical analysis of gibberellic acid involvement in the stages of soybean (Glycine max L.) nodule development

Potten¹

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Gibberellins in fungi, bacteria and lower plants: biosynthesis, function and evolution

Tudzynski¹,

Studt²,

Rojas³

2016

Annual Plant Reviews, Volume 49

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Gibberellins in Fungi, Bacteria and Lower Plants: Biosynthesis, Function and Evolution

Tudzynski

Studt

Rojas

2018

Annual Plant Reviews Online

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The rice pathogenFusarium fujikuroi, as well as two distantly related fungi,Sphaceloma manihoticolaandPhaeosphaeriaesp., contains clusters of gibberellin (GA) biosynthesis genes and produces GAs. Fungal GAs are structurally identical to those synthesised by higher plants, although the respective biosynthetic pathways, genes and enzymes differ. Besides fungi, some bacteria synthesise GAs. An operon of GA biosynthesis genes was found in the symbiotic rhizobacteriumBradyrhizobium japonicumand in otherRhizobiumspecies. This operon encodes the enzymes of GA9biosynthesis and includes a reductase or alcohol dehydrogenase gene not present in fungal clusters. Differences between genes and enzymes indicate convergent evolution of the GA biosynthesis pathway in higher plants, fungi and bacteria. In contrast to higher plants, GAs are not produced by all lower plants. Mosses or liverworts synthesiseent‐kaurenoids, but not GAs, while lycophytes and ferns synthesise GAs or GA‐related products that participate in the regulation of reproductive development.

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Gibberellin oxidase activities in Bradyrhizobium japonicum bacteroids

Cited by 30 publications

References 37 publications

Molecular genetic and biochemical analysis of gibberellic acid involvement in the stages of soybean (Glycine max L.) nodule development

Molecular genetic and biochemical analysis of gibberellic acid involvement in the stages of soybean (Glycine max L.) nodule development

Gibberellins in fungi, bacteria and lower plants: biosynthesis, function and evolution

Gibberellins in Fungi, Bacteria and Lower Plants: Biosynthesis, Function and Evolution

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