Gigapixel imaging with a novel multi-camera array microscope

Abstract

The dynamics of living organisms are organized across many spatial scales. However, current cost-effective imaging systems can measure only a subset of these scales at once. We have created a scalable multi-camera array microscope (MCAM) that enables comprehensive high-resolution recording from multiple spatial scales simultaneously, ranging from structures that approach the cellular scale to large-group behavioral dynamics. By collecting data from up to 96 cameras, we computationally generate gigapixel-scale images and movies with a field of view over hundreds of square centimeters at an optical resolution of 18 µm. This allows us to observe the behavior and fine anatomical features of numerous freely moving model organisms on multiple spatial scales, including larval zebrafish, fruit flies, nematodes, carpenter ants, and slime mold. Further, the MCAM architecture allows stereoscopic tracking of the z-position of organisms using the overlapping field of view from adjacent cameras. Overall, by removing the bottlenecks imposed by single-camera image acquisition systems, the MCAM provides a powerful platform for investigating detailed biological features and behavioral processes of small model organisms across a wide range of spatial scales.

Supertemporal Resolution Imaging of Membrane Potential via Stroboscopic Microscopy

“…It is noteworthy that SVI requires repeated measurement of the same bioelectrical event, such as triggered firing of a single AP, and is thus incapable of recording spontaneous electrical activities. Parallel recording with a multicamera array has been applied to boost the FoV in high-speed biological imaging applications, such as the motion of organisms , and Ca 2+ responses in cell ensembles . Alternatively, the acquisition rate could be increased via temporal pixel multiplexing, which involves embedding several sequential image acquisition frames into a single camera exposure via a synchronized oscillatory projector. , These techniques offer attractive avenues to achieve kilohertz voltage imaging for capturing spontaneous events.…”

High-throughput imaging across multiple spatial scales with the multi-camera array microscope (MCAM)

“…5 Other designs include up to 96 cameras. 6 Beyond its obvious advantage to simultaneously image a large FOV at high resolution, the MCAM allows different configurations for different functionalities. 5 Multi-view imaging for 3D reconstructions In this configuration, the MCAM uses lenses with a large working distance and a low image magnification.…”

Scalable Apparatus to Measure Posture and Locomotion (SAMPL): a high-throughput solution to study unconstrained vertical behavior in small animals

“…These devices lend themselves well to screens, and offer commercial options, but the range of behaviors is compressed 72 . Like other attempts to preserve high-resolution kinematic information while accommodating natural unconstrained behavior 22,[73][74][75][76][77][78] , SAMPL sits between these two extremes, joining other open-source software packages such as Stytra 79 and Zebrazoom 80 . We see SAMPL as a complementary tool.…”