Gill morphology and sodium influx in the rainbow trout (<i>Salmo gairdneri</i>) acclimated to artificial freshwater environments

Avella, Martine; Masoni, A.; Bornancin, M.; Mayer-Gostan, N.

doi:10.1002/jez.1402410202

Cited by 152 publications

(64 citation statements)

References 12 publications

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“…deionized freshwater) results in a proliferation of cells on the secondary lamellae in several teleosts (Avella et al, 1987;Perry, 1997). In some species, such as American shad (Alosa sapidissima), the number of chloride cells on the secondary lamellae is high in freshwater fish but greatly reduced in seawater fish ).…”

Section: Discussionmentioning

confidence: 99%

Influence of salinity on the localization of Na+/K+-ATPase,Na+/K+/2Cl- cotransporter (NKCC) and CFTR anion channel in chloride cells of the Hawaiian goby (Stenogobius hawaiiensis)

McCormick

Sundell

Björnsson

et al. 2003

Journal of Experimental Biology

111

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SUMMARY Na+/K+-ATPase,Na+/K+/2Cl- cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR) are the three major transport proteins thought to be involved in chloride secretion in teleost fish. If this is the case, the levels of these transporters should be high in chloride cells of seawater-acclimated fish. We therefore examined the influence of salinity on immunolocalization of Na+/K+-ATPase, NKCC and CFTR in the gills of the Hawaiian goby (Stenogobius hawaiiensis). Fish were acclimated to freshwater and 20‰ and 30‰ seawater for 10 days. Na+/K+-ATPase and NKCC were localized specifically to chloride cells and stained throughout most of the cell except for the nucleus and the most apical region, indicating a basolateral/tubular distribution. All Na+/K+-ATPase-positive chloride cells were also positive for NKCC in all salinities. Salinity caused a slight increase in chloride cell number and size and a slight decrease in staining intensity for Na+/K+-ATPase and NKCC, but the basic pattern of localization was not altered. Gill Na+/K+-ATPase activity was also not affected by salinity. CFTR was localized to the apical surface of chloride cells, and only cells staining positive for Na+/K+-ATPase were CFTR-positive. CFTR-positive cells greatly increased in number (5-fold), area stained (53%) and intensity (29%)after seawater acclimation. In freshwater, CFTR immunoreactivity was light and occurred over a broad apical surface on chloride cells, whereas in seawater there was intense immunoreactivity around the apical pit (which was often punctate in appearance) and a light subapical staining. The results indicate that Na+/K+-ATPase, NKCC and CFTR are all present in chloride cells and support current models that all three are responsible for chloride secretion by chloride cells of teleost fish.

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Section: Discussionmentioning

confidence: 99%

Influence of salinity on the localization of Na+/K+-ATPase,Na+/K+/2Cl- cotransporter (NKCC) and CFTR anion channel in chloride cells of the Hawaiian goby (Stenogobius hawaiiensis)

McCormick

Sundell

Björnsson

et al. 2003

Journal of Experimental Biology

111

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“…Chloride cells on the secondary lamellae increase in number during exposure to ion-poor water (Avella et al, 1987), suggesting an important function for these cells in ion absorption. The Na ϩ -K ϩ -2Cl Ϫ cotransporter has been hypothesized only to be in seawater chloride cells and involved in ion secretion.…”

Section: Figmentioning

confidence: 99%

Effects of Growth Hormone and Cortisol on Na+–K+–2Cl− Cotransporter Localization and Abundance in the Gills of Atlantic Salmon

Pelis

McCormick

2001

General and Comparative Endocrinology

109

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The hormones responsible for the regulation of the teleostean gill Na ؉ -K ؉ -2Cl ؊ cotransporter have not been elucidated. With Western blotting and immunocytochemistry, Na ؉ -K ؉ -2Cl ؊ cotransporter abundance and localization were examined in the gills of Atlantic salmon (Salmo salar) following 2-week treatment with growth hormone (GH; 5.0 g ⅐ g ؊1 ), cortisol (50 g ⅐ g ؊1 ), and both hormones in combination (GH؉cortisol). GH and cortisol treatments increased gill Na ؉ -K ؉ -2Cl ؊ cotransporter abundance over levels seen in controls, and both hormones together (GH؉cortisol) produced a greater effect than either hormone alone. Gill Na ؉ ,K ؉ -ATPase activity was also elevated by all three hormone treatments. Compared to controls, Na ؉ -K ؉ -2Cl ؊ cotransporter immunoreactive chloride cells on the primary filament were greater in number and size following all three treatments. Although the number of immunoreactive chloride cells on the secondary lamellae did not differ among the treatment groups, GH؉cortisol increased their size. These data indicate that GH and cortisol increase gill Na ؉ -K ؉ -2Cl ؊ cotransporter abundance through chloride cell proliferation and differentiation in the gills of Atlantic salmon and are likely the hormones responsible for Na ؉ -K ؉ -2Cl ؊ cotransporter regulation during smolting and seawater acclimation.

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“…The role of different cell types in marine fish gill epithelia has been established and cell distribution has been clearly defined (Foskett et al, 1981;Perry & Laurent, 1993). The physiological functions of the gill cells of freshwater fish, however, appear to be more complex (Evans, 1984;Perry & Wood, 1985;Avella et al, 1987;Perry & Laurent, 1989, 1993Brown, 1992;Goss et al, 1992aGoss et al, , b, 1994. Freshwater environments vary from almost distilled to high ion concentration waters and, therefore, the epithelial gill cell types in freshwater fish show morphological and functional adaptations that allow the species to live in such environments.…”

Section: Introductionmentioning

confidence: 99%

Epithelial gill cells in the armored catfish, Hypostomus cf. plecostomus (Loricariidae)

Fernandes

Perna-Martins

2001

Rev. Bras. Biol.

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Epithelial gill cell morphology and distribution were investigated in the armored catfish, Hypostomus cf. plecostomus, which lives in soft ion-poor Brazilian freshwaters. Pavement cells are the most abundant type of cell on both filament and lamellar epithelia and there are a great number of mucous and chloride cells between them. Mucous cells are almost covered by adjacent pavement cells and have large packed granules showing electrondense differences. No mucous cells were found on the lamellar epithelium. Chloride cell were distributed throughout both epithelia and usually have large apical surface facing the external medium and may exhibit short and sparsely distributed microvilli. The presence of chloride cells on the lamellar epithelium may be an adaptation to low ion concentrations in the water, allowing for improved ion-transport capacity of the gill. The large size of these cells increases the water-blood barrier and may affect the transference of respiratory gases. However, the negative effect on the respiratory process may be minimized by this species' ability to resort to atmospheric air to fulfill its oxygen requirements.Key words: gills, epithelium, teleost, loricariid, Hypostomus cf. plecostomus. RESUMOEpitélio branquial de cascudo, Hypostomus cf. plecostomus (Loricariidae)

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Gill morphology and sodium influx in the rainbow trout (Salmo gairdneri) acclimated to artificial freshwater environments

Cited by 152 publications

References 12 publications

Influence of salinity on the localization of Na+/K+-ATPase,Na+/K+/2Cl- cotransporter (NKCC) and CFTR anion channel in chloride cells of the Hawaiian goby (Stenogobius hawaiiensis)

Influence of salinity on the localization of Na+/K+-ATPase,Na+/K+/2Cl- cotransporter (NKCC) and CFTR anion channel in chloride cells of the Hawaiian goby (Stenogobius hawaiiensis)

Effects of Growth Hormone and Cortisol on Na+–K+–2Cl− Cotransporter Localization and Abundance in the Gills of Atlantic Salmon

Epithelial gill cells in the armored catfish, Hypostomus cf. plecostomus (Loricariidae)

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