Gill Na,K-ATPase in the spiny lobster Palinurus elephas and other marine osmoconformers

Lucu, Čedomil; Devescovi, Massimo; Skaramuca, Boško; Kožul, Valter

doi:10.1016/s0022-0981(99)00179-3

Cited by 46 publications

(27 citation statements)

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“…This has been accomplished, starting from marine ancestors, through a keen capacity to withstand salinity fluctuations in estuaries or to absorb salt from freshwater, or even to tolerate air exposure, especially in crabs (Kirschner, '91;Péqueux, '95;Freire et al, 2008a,b). Selected osmoregulatory strategies underlying this range of distribution follow a pattern: osmoconformation in sea water, osmoregulation in fresh water, and a variable behavior in estuaries (Kirschner, '91, 2004;Péqueux, '95;Lucu et al, 2000;Freire et al, 2008a,b). It should be noted that this is a general invertebrate pattern, not exclusive to the decapod crustaceans (Kirschner, '91, 2004;Péqueux, '95;Freire et al, 2008a,b), as there is no way out from osmoregulation (AER) in fresh water.…”

Section: Discussionmentioning

confidence: 99%

“…Time of exposure to the salinity challenge is a key factor in determining what type of responses are available and will be elicited in the animal. Although sometimes clear osmoregulatory or osmoconforming patterns can be visualized, the limits/boundaries between both types of behavior are not always clearly defined (see Péqueux, '95;Lucu et al, 2000;Freire et al, 2003Freire et al, , 2008a. Most osmoregulators, depending on the salinity and time of exposure, may start to display osmoconforming behavior, until eventually reaching their limit of tolerance.…”

mentioning

confidence: 99%

“…On the other hand, stenohaline regulators or conformers presumably display a relatively lower capacity to regulate cell volume, which then results in a narrower range of tolerance to salinity variation (Gilles, '73;Gilles and Péqueux, '83;Kirschner, '91;Péqueux, '95;Freire et al, 2008b). Uncompensated water fluxes to/from the cells then lead to irreversible cell swelling or shrinking (Gilles, '73;Burton and Feldman, '82;Herrera et al, '89;Dragolovich and Pierce, '92;Deaton, '97;Lucu et al, 2000;Souza and Scemes, 2000;Freire et al, 2008b). Cell volume regulation or IIR (Florkin, '62;Péqueux, '95;Freire et al, 2008b) is a well-conserved process, involving transmembrane transport mechanisms in species widely separated by the evolutionary process (Kinne, '93;Péqueux, '95;Wehner et al, 2003;Augusto et al, 2007b;Hoffmann et al, 2009).…”

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confidence: 99%

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Do osmoregulators have lower capacity of muscle water regulation than osmoconformers? A study on decapod crustaceans

et al. 2010

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Decapod crustaceans occupy various aquatic habitats. In freshwater they are osmoregulators, while marine species are typically osmoconformers. Freshwater crustaceans are derived from marine ancestors. The hypothesis tested here was that osmoregulators, which can rely on salt transport by interface epithelia to prevent extracellular disturbance, would have a lower capacity of tissue water regulation when compared with osmoconformers. Four species of decapod crustaceans (the marine osmoconformer crab Hepatus pudibundus, and three osmoregulators of different habitats) have been exposed in vivo to a salinity challenge, for up to 24 hr. Osmoregulators were: the estuarine shrimp Palaemon pandaliformis, the diadromous freshwater shrimp Macrobrachium acanthurus, and the hololimnetic red crab Dilocarcinus pagei. H. pudibundus displayed hemolymph dilution already after 0.5 hr in 25 per thousand, reaching approximately 30% reduction in osmolality, but its muscle degree of hydration did not increase. To make the different in vivo salinity challenges directly comparable, the ratio between the maximum change in muscle hydration with respect to the control value measured for the species and the maximum change in hemolymph osmolality was calculated (x 1,000): H. pudibundus (25 per thousand, 8.1% kg H(2)O/mOsm x 10(3))>P. pandaliformis (2 per thousand, 9.2)>M. acanthurus (30 per thousand, 12.6)>P. pandaliformis (35 per thousand, 16.7)>D. pagei (7 per thousand, 60.4). Muscle slices submitted in vitro to a 30% osmotic challenge confirmed in vivo results. Thus, the estuarine/freshwater osmoregulators displayed a lower capacity to hold muscle tissue water than the marine osmoconformer, despite undergoing narrower variations in hemolymph osmolality.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Do osmoregulators have lower capacity of muscle water regulation than osmoconformers? A study on decapod crustaceans

et al. 2010

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“…Crabs can actively uptake or excrete ions upon salinity change, and the gills play a major role among the osmoregulatory organs. Several enzymes are involved in the osmoregulatory process, including Na (Lucu et al, 2000). For example, in the marine crab Carcinus maenas, NKA is the driving force for Na + uptake in dilute seawater (Weihrauch et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

V-type H+-ATPase and Na+,K+-ATPase in the gills of 13 euryhaline crabs during salinity acclimation

Tsai

Lin

2007

Journal of Experimental Biology

127

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SUMMARY Because of their diverse habitats, crabs are excellent experimental species to study owing to the morphological changes and physiological adaptation that occur during their terrestrial invasion. Their hemolymphic osmoregulation in brackish water is crucial for a successful terrestrial invasion. Crabs can actively uptake or excrete ions upon salinity change, and the gills play a major role among the osmoregulatory organs. Several enzymes are involved in the osmoregulatory process, including Na+,K+-ATPase and V-type H+-ATPase (V-H+-ATPase). Na+,K+-ATPase is the driving force in establishing an ion gradient across the epithelial cell membrane in marine crabs. It has been reported that the osmoregulatory mechanisms in freshwater crabs are different from those in marine ones, suggesting that the driving force may come from V-H+-ATPase by generating the H+ ion gradient to facilitate the ion flow. Thirteen crab species from two families were used in this study. These crabs lived in five different habitats, including marine,intertidal, bimodal, freshwater and terrestrial habitats. The distribution of V-H+-ATPase in the 13 euryhaline crabs was revealed by histochemistry. V-H+-ATPase was localized in the apical region in crabs that could survive in the freshwater environment. We found that the freshwater and terrestrial crabs with stable Na+,K+-ATPase activity during salinity changes tended to have an apical V-H+-ATPase, whereas the intertidal ones with varying Na+,K+-ATPase activity showed a cytoplasmic V-H+-ATPase distribution. Finally, in Uca formosensis, a crab that had stable Na+,K+-ATPase activity, a significant difference in V-H+-ATPase activity between salinities was found. In conclusion, the hypothesis that V-H+-ATPase plays a crucial role in the freshwater adaptation of crabs is supported by our systemic investigation on 13 euryhaline crabs.

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“…Esse valor encontra-se na faixa relatada para homogenados de brânquias de outras espécies de Callinectes, compreendidos de 35 a 225 U/mg (Lovett & Watts, 1995;Piller et al, 1995;Lucu et al, 2000), porém é cerca de três vezes menor que o reportado para C. danae (306.9 ± 17.2 U mg -1 ). Esse resultado já era esperado, pois em salinidades menores existe uma maior necessidade de transporte de íons sódio e potássio.…”

Section: Caracterização Da Atividade Atpase Da (Naunclassified

Caracterização cinética da (Na+,K+)-ATPase da fração microsomal do tecido branquial do siri Callinectes ornatus ordway, 1863 (Crustacea, Portunidae)

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Agradeço por seu precioso tempo dedicado a minha formação, durante o período que estive em seu laboratório. Sinto-me honrada e feliz pelas oportunidades de aprendizado e constantes discussões científicas que me foram proporcionadas por ele, apesar das "divergências". As palavras são poucas para expressar minha profunda gratidão. Muito obrigada..... Aos meus familiares pelo apoio, incentivo, confiança e amor. Muito obrigado por vocês existirem na minha vida, amo todos vocês. AGRADECIMENTOS AGRADECIMENTOS AGRADECIMENTOS AGRADECIMENTOSAos colegas e amigos do laboratório Nara, Kelly Cristina, Luciana, Rúbia, Flávio, Carlos, Ana Lúcia, Sandra, Camila, Ricardo e aos colegas dos laboratórios de bioquímica do departamento de química, que conviveram comigo durante a realização deste trabalho.A Ivana Ap. Borin e Nilton R. Alves, tanto pela dedicação e suporte técnico, quanto pela profunda prova de companheirismo recebida durante esse período.A Lâmia, Isabel, Emerson, André, Sonia O., Maria Inês, Sonia M. e Denise pela eficiência, atenção e paciência dedicadas.A todos os Docentes e

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Gill Na,K-ATPase in the spiny lobster Palinurus elephas and other marine osmoconformers

Cited by 46 publications

References 30 publications

Do osmoregulators have lower capacity of muscle water regulation than osmoconformers? A study on decapod crustaceans

Do osmoregulators have lower capacity of muscle water regulation than osmoconformers? A study on decapod crustaceans

V-type H+-ATPase and Na+,K+-ATPase in the gills of 13 euryhaline crabs during salinity acclimation

Caracterização cinética da (Na+,K+)-ATPase da fração microsomal do tecido branquial do siri Callinectes ornatus ordway, 1863 (Crustacea, Portunidae)

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