Gingival crevicular fluid and salivary HIF‐1α, VEGF, and TNF‐α levels in periodontal health and disease

Afacan, Beral; Öztürk, Veli Özgen; Paşalı, Çiğdem; Bozkurt, Emir; Köse, Timur; Emingil, Gülnur

doi:10.1002/jper.18-0412

Cited by 74 publications

(70 citation statements)

References 50 publications

(116 reference statements)

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“…17 Increased HIF-1 protein expression levels have been shown in the gingival tissue of patients with periodontitis. 18,19 Recently, two clinical studies by Afacan et al 20 and Tayman et al 21 have reported that G-AgP and chronic periodontitis patients had significantly higher GCF HIF-1 levels than periodontally healthy controls. These findings suggest a possible association of HIF-1 with the pathophysiology of periodontal inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…These findings suggest a possible association of HIF-1 with the pathophysiology of periodontal inflammation. [18][19][20][21] As HIF-1 , under hypoxic condition, upregulates primer angiogenic mediator vascular endothelial growth factor (VEGF) 22,23 which is closely related to periodontal inflammation, wound healing and alveolar bone remodeling, [24][25][26] TNF-increases HIF-1 and its downstream effector VEGF in an oxygenindependent manner. 27,28 It has been reported that protein expressions and GCF total amounts of HIF-1 may be associated with VEGF and TNF-levels in patients with periodontitis, which in turn implies that TNF-/HIF-1 /VEGF pathway may involved in periodontal inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…27,28 It has been reported that protein expressions and GCF total amounts of HIF-1 may be associated with VEGF and TNF-levels in patients with periodontitis, which in turn implies that TNF-/HIF-1 /VEGF pathway may involved in periodontal inflammation. 18,20 The management of G-AgP still represents a challenge in periodontal practice. The use of topical and systemic antimicrobials as an adjunct to scaling and root planing (SRP) have been investigated in patients with G-AgP.…”

Section: Introductionmentioning

confidence: 99%

“…Increased HIF‐1α protein expression levels have been shown in the gingival tissue of patients with periodontitis 18,19 . Recently, two clinical studies by Afacan et al 20 . and Tayman et al 21 .…”

Section: Introductionmentioning

confidence: 99%

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Effect of non‐surgical periodontal treatment on gingival crevicular fluid hypoxia inducible factor‐1 alpha, vascular endothelial growth factor and tumor necrosis factor‐alpha levels in generalized aggressive periodontitis patients

Afacan

Yücel

Paşalı

et al. 2020

Journal of Periodontology

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Background: Hypoxia-inducible angiogenic pathway involving hypoxia inducible factor-1 alpha (HIF-1), vascular endothelial growth factor (VEGF) and tumor necrosis factor-alpha (TNF-) may regulate several biological processes related to inflammation. The present study aimed to assess the effect of non-surgical periodontal treatment on gingival crevicular fluid (GCF) HIF-1 , VEGF, and TNFlevels in generalized aggressive periodontitis (G-AgP). Methods: Twenty G-AgP patients and 20 periodontally healthy individuals were included. G-AgP patients received scaling and root planning (SRP), per quadrant at a 1-week-interval, performed with ultrasonic and periodontal hand instruments. GCF samples were collected and clinical periodontal parameters including probing depth, clinical attachment level, gingival index and plaque index were recorded at baseline, 1 and 3 months after treatment. Biomarker levels in GCF were analyzed by ELISA. Results: At baseline all clinical parameters and GCF HIF-1 , VEGF, and TNFlevels were significantly higher in G-AgP patients compared to healthy control (P < 0.05). All clinical parameters improved over the 3-month-period in G-AgP patients (P < 0.05). GCF HIF-1 levels in G-AgP reduced at 1 and 3 months posttreatment, however, this did not reach to statistical significance (P > 0.05). GCF VEGF and TNF-levels remained unchanged throughout the study period (P > 0.05). This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of non‐surgical periodontal treatment on gingival crevicular fluid hypoxia inducible factor‐1 alpha, vascular endothelial growth factor and tumor necrosis factor‐alpha levels in generalized aggressive periodontitis patients

Afacan

Yücel

Paşalı

et al. 2020

Journal of Periodontology

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“…Even though a broad range of methods has been used to analyze protein signatures in saliva for the characterization of G‐AgP, spanning from immunoassays to mass spectrometric–based proteomics, [ 29‐31 ] most of them have by far failed to reach the clinical setting, due their laborious and complex configuration.…”

mentioning

confidence: 99%

Salivary Fingerprinting of Periodontal Disease by Infrared‐ATR Spectroscopy

Beyer‐Hans

Sigrist

Silbereisen

et al. 2020

Proteomics Clinical Apps

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Purpose: Periodontal diseases, the most common chronic inflammatory diseases in humans, do not only affect tooth-supporting tissues but also other body parts by contributing to the development of life-threatening conditions. Since currently available diagnostic methods in periodontics lack the ability to identify patients at high risk for periodontal disease progression, development of innovative, non-invasive, rapid detection methods for diagnosing periodontal diseases is needed. This study aims to assess the potential of infrared attenuated total reflection (IR-ATR) spectroscopy to detect differences in composition of saliva supernatant in non-periodontitis individuals (control) and patients with generalized aggressive periodontitis (G-AgP). Experimental Design: IR-ATR is performed with a wavelength interval from 1230 to 1180 cm −1 , analyzed with a simple subtraction in absorbance data. Results: Ten samples show in the analysis of variance of the two data sets a true difference (99.8%). A principal component analysis (PCA) is able to discriminate between G-AgP and control groups. Conclusion and Clinical Relevance:This study demonstrates for the first time that IR-ATR spectroscopy is a promising tool for the analysis of saliva supernatant for the diagnosis of periodontitis, and potentially other periodontal conditions. IR-ATR spectroscopy holds the potential to be miniaturized and utilized as a non-invasive screening test.Periodontal diseases are the most common chronic inflammatory diseases in humans, and affect the tooth-supporting tissues. Moreover, recent evidence has indicated that periodontal diseases could contribute to the development of life-threating conditions. [1,2] Of the several forms of periodontitis, generalized aggressive periodontitis (G-AgP) is the most severe,

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Gingival crevicular fluid periodontal ligament‐associated protein‐1, sclerostin, and tumor necrosis factor‐alpha levels in periodontitis

Gür

Afacan

Çevik

et al. 2023

Journal of Periodontology

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BackgroundIn periodontitis, the equilibrium between bone formation and resorption skews in favor of bone loss. Periodontal ligament‐associated protein‐1 (PLAP‐1) and sclerostin play a significant role in the suppression of bone formation. Tumor necrosis factor‐alpha (TNF‐α) is a central proinflammatory cytokine related to periodontal bone loss. This study aims to assess gingival crevicular fluid (GCF) PLAP‐1, sclerostin, and TNF‐α levels in individuals with periodontal disease.MethodsSeventy‐one individuals diagnosed with generalized stage III grade C periodontitis (n = 23), gingivitis (n = 24), and periodontal health (n = 24) were included in the study. Full‐mouth clinical periodontal measurements were performed. PLAP‐1, sclerostin, and TNF‐α total amounts in GCF were quantified by ELISA. Nonparametric methods were used for the data analyses.ResultsPeriodontitis group exhibited significantly higher GCF PLAP‐1, sclerostin and TNF‐α levels compared with gingivitis and periodontally healthy groups (p < 0.05). GCF PLAP‐1 and TNF‐α levels of gingivitis group were higher than healthy controls (p < 0.05) whereas GCF sclerostin levels were similar in two groups (p > 0.05). Significant positive correlations were found between GCF PLAP‐1, sclerostin and TNF‐α levels and all clinical parameters (p < 0.01).ConclusionsTo our knowledge, this is the first study showing GCF PLAP‐1 levels in periodontal health and disease. Increased GCF PLAP‐1 and sclerostin levels and their correlations with TNF‐α in periodontitis imply that those molecules might be involved in the pathogenesis of periodontal disease. Further studies in larger mixed cohorts are needed to enlighten the possible role of PLAP‐1 and sclerostin in periodontal bone loss.

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Gingival crevicular fluid and salivary HIF‐1α, VEGF, and TNF‐α levels in periodontal health and disease

Cited by 74 publications

References 50 publications

Effect of non‐surgical periodontal treatment on gingival crevicular fluid hypoxia inducible factor‐1 alpha, vascular endothelial growth factor and tumor necrosis factor‐alpha levels in generalized aggressive periodontitis patients

Effect of non‐surgical periodontal treatment on gingival crevicular fluid hypoxia inducible factor‐1 alpha, vascular endothelial growth factor and tumor necrosis factor‐alpha levels in generalized aggressive periodontitis patients

Salivary Fingerprinting of Periodontal Disease by Infrared‐ATR Spectroscopy

Gingival crevicular fluid periodontal ligament‐associated protein‐1, sclerostin, and tumor necrosis factor‐alpha levels in periodontitis

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