Gingival Crevicular Fluid Levels of Neopterin in Healthy Subjects and in Patients With Different Periodontal Diseases

Pradeep, A.R.; Kumar, Magesh; Ramachandraprasad, M.V.; Chowdhry, Shikha

doi:10.1902/jop.2007.070096

Cited by 28 publications

(30 citation statements)

References 20 publications

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“…In our study also salivary neopterin levels were increased in both the groups and also decreased levels were seen in both the groups after NSPT, but significant reduction was seen in the pre-menopause group. This study result of reduced neopterin levels after NSPT are consistent with the study by Pradeep et al34. The neopterin levels were correlated with the intensity of disease.…”

Section: Discussionsupporting

confidence: 93%

Comparative Evaluation of Salivary Neopterin Levels and Its Effects to Periodontal Therapy in Pre- and Post-Menopausal Women

et al. 2017

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ObjectivesNeopterin is a valuable diagnostic biomarker, which is elevated in inflammatory conditions like periodontitis, that is characterized by destruction of the supporting structures of the teeth. Among the biomarkers, neopterin occurs in body fluids, and acts as a diagnostic marker for present and future disease activity.MethodsThirty female subjects with chronic periodontitis, mean age 50 years (40-60 years) were included in this study. Depending upon their menstrual history, subjects were categorized into two groups of fifteen each. Group I 15 pre-menopausal women, and Group II 15 post-menopausal women. Saliva was collected, and neopterin levels were assessed by enzyme-linked immunosorbent assay in both the groups, at base line and after three months of nonsurgical periodontal therapy (NSPT). Periodontal parameters like pocket probing depth (PD) and Russell's periodontal disease index (PDI) were assessed before treatment as well as after three months of scaling and root planning.ResultsIntra group analysis showed significant markdown in the mean values of all the parameters from baseline to three months (P < 0.001), for all patients. The intergroup comparison, from baseline to 3 months also showed no significant change in PD and PDI values, but there was a statistically significant difference in the salivary neopterin levels (P = 0.04).ConclusionsNeopterin levels were found to be reduced in three months after NSPT in both the groups, suggesting that the NSPT is the gold standard therapy, and also that neopterin levels in saliva can be used as an indicator to identify periodontal inflammation and destruction.

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Section: Discussionsupporting

confidence: 93%

Comparative Evaluation of Salivary Neopterin Levels and Its Effects to Periodontal Therapy in Pre- and Post-Menopausal Women

et al. 2017

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“…As a primary marker of macrophage activation, NP is an important indicator of AS progression . Furthermore, a recent study found that NP increased in parallel with the severity of inflammatory disease and its levels of GCF may be useful as an indicator of periodontal inflammation and the host response . Serum NP can better reflect systemic inflammation, compared to saliva NP which can only reflect local inflammation in a person's mouth.…”

Section: Discussionmentioning

confidence: 99%

Decreased circulating neopterin is associated with increased arterial elasticity: a beneficial role of periodontal treatment

Ren

et al. 2016

Australian Dental Journal

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“…To stratify the chronic gingivitis and chronic periodontitis patients, the clinical periodontal parameters from average 6 sites per individual were assessed at the initial examination for mean probing depth (PD), clinical attachment loss (CAL), bleeding upon probing (BOP), and the gingival index (GI) (Table 1) [15]. According to the criteria and complications, we divided the samples into normal (N), chronic gingivitis (GV), moderate periodontitis (MP), and severe periodontitis (SP) and collected GCF with a sterile gel loading tip (DASLAB, Spain).…”

Section: Methodsmentioning

confidence: 99%

Identification of azurocidin as a potential periodontitis biomarker by a proteomic analysis of gingival crevicular fluid

et al. 2011

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BackgroundThe inflammatory disease periodontitis results in tooth loss and can even lead to diseases of the whole body if not treated. Gingival crevicular fluid (GCF) reflects the condition of the gingiva and contains proteins transuded from serum or cells at inflamed sites. In this study, we aimed to discover potential protein biomarkers for periodontitis in GCF proteome using LC-MS/MS.ResultsWe identified 305 proteins from GCF of healthy individuals and periodontitis patients collected using a sterile gel loading tip by ESI-MS/MS coupled to nano-LC. Among these proteins, about 45 proteins were differentially expressed in the GCF proteome of moderate periodontitis patients when compared to the healthy individuals. We first identified azurocidin in the GCF, but not the saliva, as an upregulated protein in the periodontitis patients and verified its increased expression during periodontitis by ELISA using the GCF of the classified periodontitis patients compared to the healthy individuals. In addition, we found that azurocidin inhibited the differentiation of bone marrow-derived macrophages to osteoclasts.ConclusionsOur results show that GCF collection using a gel loading tip and subsequent LC-MS/MS analysis following 1D-PAGE proteomic separation are effective for the analysis of the GCF proteome. Our current results also suggest that azurocidin could be a potential biomarker candidate for the early detection of inflammatory periodontal destruction by gingivitis and some chronic periodontitis. Our data also suggest that azurocidin may have an inhibitory role in osteoclast differentiation and, thus, a protective role in alveolar bone loss during the early stages of periodontitis.

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Gingival Crevicular Fluid Levels of Neopterin in Healthy Subjects and in Patients With Different Periodontal Diseases

Abstract: Neopterin increased in parallel with the severity of inflammatory disease. Its levels in GCF may be potentially useful as an indicator of periodontal inflammation and the host response.

Cited by 28 publications

References 20 publications

Comparative Evaluation of Salivary Neopterin Levels and Its Effects to Periodontal Therapy in Pre- and Post-Menopausal Women

Comparative Evaluation of Salivary Neopterin Levels and Its Effects to Periodontal Therapy in Pre- and Post-Menopausal Women

Decreased circulating neopterin is associated with increased arterial elasticity: a beneficial role of periodontal treatment

Identification of azurocidin as a potential periodontitis biomarker by a proteomic analysis of gingival crevicular fluid

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