Gingival mesenchymal stem cells as an alternative source to bone marrow mesenchymal stem cells in regeneration of bone defects: In vivo study

Al-Qadhi, Gamilah; Solimán, M. K.; Aboushady, Iman M.; Rashed, Laila A.

doi:10.1016/j.tice.2019.101325

Cited by 27 publications

(32 citation statements)

References 52 publications

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“…The transplantation of GMSCs and BMSCs loaded onto the NanoBone showed better bone regeneration compared to a scaffold without cells. Interestingly, no difference was found in the new bone formed by the scaffolds loaded with GMSCs or BMSCs, indicating that GMSCs represent a good alternative to BMSCs [ 94 ].…”

Section: Biomaterials and Mesenchymal Stem Cells For Bone Tissue Engineeringmentioning

confidence: 99%

Oral Bone Tissue Regeneration: Mesenchymal Stem Cells, Secretome, and Biomaterials

Gugliandolo

Fonticoli

Trubiani

et al. 2021

IJMS

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In the last few decades, tissue engineering has become one of the most studied medical fields. Even if bone shows self-remodeling properties, in some cases, due to injuries or anomalies, bone regeneration can be required. In particular, oral bone regeneration is needed in the dentistry field, where the functional restoration of tissues near the tooth represents a limit for many dental implants. In this context, the application of biomaterials and mesenchymal stem cells (MSCs) appears promising for bone regeneration. This review focused on in vivo studies that evaluated bone regeneration using biomaterials with MSCs. Different biocompatible biomaterials were enriched with MSCs from different sources. These constructs showed an enhanced bone regenerative power in in vivo models. However, we discussed also a future perspective in tissue engineering using the MSC secretome, namely the conditioned medium and extracellular vesicles. This new approach has already shown promising results for bone tissue regeneration in experimental models.

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Section: Biomaterials and Mesenchymal Stem Cells For Bone Tissue Engineeringmentioning

confidence: 99%

Oral Bone Tissue Regeneration: Mesenchymal Stem Cells, Secretome, and Biomaterials

Gugliandolo

Fonticoli

Trubiani

et al. 2021

IJMS

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“…For instance, Ansari S et al reported that GMSCs encapsulated in alginate hydrogel without gelatin methacryloyl (GelMA) underwent osteogenic differentiation without the aid of additional growth factors, suggesting the possibility to control the fate of encapsulated MSCs within hydrogels by tuning the mechanical properties of the matrix (131). Most recently, a study has shown that local application of GMSCs can promote long bone regeneration (132). Using a criticalsized tibiae defect model in rabbit, Al-Qadhi G et al showed that local transplantation NanoBone scaffolds loaded with GMSCs or BMSCs significantly enhanced the new bone formation as compared to the unloaded scaffolds, whereby GMSCs and BMSCs exhibited similar degree of bone regenerative potentials in bone defects in rabbits' tibiae, suggesting that GMSCs could be a comparable alternative source to BMSCs for bone regeneration (132).…”

Section: Bone and Cartilage Regenerationmentioning

confidence: 99%

“…Most recently, a study has shown that local application of GMSCs can promote long bone regeneration (132). Using a criticalsized tibiae defect model in rabbit, Al-Qadhi G et al showed that local transplantation NanoBone scaffolds loaded with GMSCs or BMSCs significantly enhanced the new bone formation as compared to the unloaded scaffolds, whereby GMSCs and BMSCs exhibited similar degree of bone regenerative potentials in bone defects in rabbits' tibiae, suggesting that GMSCs could be a comparable alternative source to BMSCs for bone regeneration (132).…”

Section: Bone and Cartilage Regenerationmentioning

confidence: 99%

Gingiva-Derived Mesenchymal Stem Cells: Potential Application in Tissue Engineering and Regenerative Medicine - A Comprehensive Review

Kim

Lee

et al. 2021

Front. Immunol.

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A unique subpopulation of mesenchymal stem cells (MSCs) has been isolated and characterized from human gingival tissues (GMSCs). Similar to MSCs derived from other sources of tissues, e.g. bone marrow, adipose or umbilical cord, GMSCs also possess multipotent differentiation capacities and potent immunomodulatory effects on both innate and adaptive immune cells through the secretion of various types of bioactive factors with immunosuppressive and anti-inflammatory functions. Uniquely, GMSCs are highly proliferative and have the propensity to differentiate into neural cell lineages due to the neural crest-origin. These properties have endowed GMSCs with potent regenerative and therapeutic potentials in various preclinical models of human disorders, particularly, some inflammatory and autoimmune diseases, skin diseases, oral and maxillofacial disorders, and peripheral nerve injuries. All types of cells release extracellular vesicles (EVs), including exosomes, that play critical roles in cell-cell communication through their cargos containing a variety of bioactive molecules, such as proteins, nucleic acids, and lipids. Like EVs released by other sources of MSCs, GMSC-derived EVs have been shown to possess similar biological functions and therapeutic effects on several preclinical diseases models as GMSCs, thus representing a promising cell-free platform for regenerative therapy. Taken together, due to the easily accessibility and less morbidity of harvesting gingival tissues as well as the potent immunomodulatory and anti-inflammatory functions, GMSCs represent a unique source of MSCs of a neural crest-origin for potential application in tissue engineering and regenerative therapy.

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“…A total of 26 studies were selected for the current systematic review. Of these, 20 in vitro were culture studies [44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63] and 6 were in vivo studies [64][65][66][67][68][69]. All papers were published in the English language, and the date of publication started in 2009.…”

Section: Characteristics Of In Vitro Included Studiesmentioning

confidence: 99%

“…Due to limitations in the number of animal studies comparing GMSCs with other sources, the authors decided to include animal studies that compare GMSCs with the control groups as well. In addition to negative controls (scaffolds without stem cells) that were used in all in vivo studies, GMSCs were compared with BMSCs [48] and with PDLSCs and BMSCs [66]. Different animal species were used to create the bony defect, rabbits [48], mice [66][67][68], dogs [69], and rats [52].…”

Section: Characteristics Of In Vivo Studiesmentioning

confidence: 99%

The Gingiva from the Tissue Surrounding the Bone to the Tissue Regenerating the Bone: A Systematic Review of the Osteogenic Capacity of Gingival Mesenchymal Stem Cells in Preclinical Studies

Al-Qadhi

Aboushady

Al-Sharabi

2021

Stem Cells International

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The current review aims to systematically assess the osteogenic capacity of gingiva-derived mesenchymal stem cells (GMSCs) in preclinical studies. A comprehensive electronic search of PubMed, Embase, Web of Science, and Scopus databases, as well as a manual search of relevant references, was performed in June 2020 without date or language restrictions. Eligibility criteria were the following: studies that compared mesenchymal stem cells (MSCs) derived from the gingiva with other MSC sources (in vitro or in vivo) or cell-free scaffold (in vivo) and studies that reported at least one of the following outcomes: osteogenic potential and new bone formation for in vitro and in vivo, respectively. Moreover, the assessment of included studies was conducted using appropriate guidelines. From 646 initial retrieved studies, 35 full-text articles were subjected to further screening and 26 studies were selected (20 in vitro studies and 6 in vivo studies). GMSCs showed great proliferation capacity and expressed recognized mesenchymal stem cell markers, particularly CD90. In vitro, MSC sources including GMSCs were capable of undergoing osteogenic differentiation with less ability in GMSCs, while most in vivo studies confirmed the capacity of GMSCs to regenerate bony defects. Concerning the assessment of methodological quality, in vitro studies met the relevant guideline except in five areas: the sample size calculation, randomization, allocation concealment, implementation, and blinding, and in vivo publications had probably low risk of bias in most domains except in three areas: allocation concealment, attrition, and blinding items.

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Gingival mesenchymal stem cells as an alternative source to bone marrow mesenchymal stem cells in regeneration of bone defects: In vivo study

Cited by 27 publications

References 52 publications

Oral Bone Tissue Regeneration: Mesenchymal Stem Cells, Secretome, and Biomaterials

Oral Bone Tissue Regeneration: Mesenchymal Stem Cells, Secretome, and Biomaterials

Gingiva-Derived Mesenchymal Stem Cells: Potential Application in Tissue Engineering and Regenerative Medicine - A Comprehensive Review

The Gingiva from the Tissue Surrounding the Bone to the Tissue Regenerating the Bone: A Systematic Review of the Osteogenic Capacity of Gingival Mesenchymal Stem Cells in Preclinical Studies

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