Ginkgo Biloba Ameliorates Subfertility Induced by Testicular Ischemia/Reperfusion Injury in Adult Wistar Rats: A Possible New Mitochondrial Mechanism

Abstract

Testicular torsion, a surgical emergency, could affect the endocrine and exocrine testicular functions. This study demonstrates histopathological and physiological effects of testicular ischemia/perfusion (I/R) injury and the possible protective effects of Ginkgo biloba treatment. Fifty adult male Wistar rats, 180–200 gm, were randomly divided into sham-operated, Gingko biloba supplemented, ischemia only, I/R, and Gingko biloba treated I/R groups. Overnight fasted rats were anaesthetized by Pentobarbital; I/R was performed by left testis 720° rotation in I/R and treated I/R groups. Orchiectomy was performed for histopathological studies and detection of mitochondrial NAD+. Determination of free testosterone, FSH, TNF-α, and IL1-β in plasma was performed. Plasma-free testosterone was significantly decreased, while plasma FSH, TNF-α, IL-1β, and testicular mitochondrial NAD+ were significantly increased in I/R group compared to control group. These parameters were reversed in Gingko biloba treated I/R group compared to I/R group. I/R caused marked testicular damage and increased APAF-1 in the apoptotic cells which were reversed by Ginkgo biloba treatment. It could be concluded that I/R caused subfertility induced by apoptosis and oxidative stress manifested by the elevated testicular mitochondrial NAD+, which is considered a new possible mechanism. Also, testicular injury could be reduced by Gingko biloba administration alone.

Paeonol protects against testicular ischaemia‐reperfusion injury in rats through inhibition of oxidative stress and inflammation

“…In agreement with the present study, several previous reports (Ahmed, Lasheen, & El-Zawahry, 2016;Arabaci Tamer et al, 2018;Zheng, Shao, Wu, Shen, & Lin, 2018) reported similar findings regarding the ability of testicular IR to increase the proinflammatory cytokines TNF-α, IL-1β and IL-6 suggesting a role for these cytokines in the IR testicular injury. The current results showed that paeonol exerted anti-inflammatory effects on IR testes by downregulating TNF-α gene expression as well as IL-1β and IL-6 protein expression.…”

The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity

“…The plasma testosterone levels were measured as described in previous studies using testosterone ELISA kits (Abcam, Inc., Cambridge, UK) for rats according to the manufacturer’s instructions [ 3 , 24 , 25 ]. The assay is based on competitive ELISA where testosterone in the sample competes with the added testosterone-horseradish peroxidase (HRP) for antibody binding.…”

Effects of Ginkgo biloba on Diseases Related to Oxidative Stress

“…The results suggest that EGB761 can be a protective and therapeutic agent against the male reproductive toxicity induced through electromagnetic radiation and through aluminum, besides lessening the alterations caused by ischemia in the testicular tissue, helping to treat the infertility in such cases. [28,67,68] Colorectal cancer The results showed that EGB761 has antitumor activity, and it can help to prevent the progression of the colorectal cancer.…”