Ginkgo biloba exocarp extracts induces autophagy in Lewis lung cancer cells involving AMPK / mTOR / p70S6k signaling pathway

Cao, Chengjie; Han, Demin; Su, Ya; Ge, Yu; Chen, Huasheng; Xu, Anjian

doi:10.1016/j.biopha.2017.07.036

Cited by 27 publications

(16 citation statements)

References 35 publications

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“…It has been shown that paeonol (2'-hydroxy-4'-methoxyacetophenone), isolated from the Moutan Cortex, can up-regulate autophagy by activating the AMPK/mTOR signaling pathway, and the autophagy inhibitor CQ notably attenuates paeonol-induced autophagy . Cao et al proved that Ginkgo Semen leaf extract can restore autophagy through inhibition of mTOR signaling (Cao et al, 2017). Based on this evidence, we postulate that JZ-1 protects against HSV-2 by inducing autophagy, a supposition confirmed in our later experiments.…”

Section: Discussionsupporting

confidence: 80%

The Chinese herbal prescription JZ-1 induces autophagy to protect against herpes simplex Virus-2 in human vaginal epithelial cells by inhibiting the PI3K/Akt/mTOR pathway

Shao

Liu

Wang

et al. 2020

Journal of Ethnopharmacology

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Section: Discussionsupporting

confidence: 80%

The Chinese herbal prescription JZ-1 induces autophagy to protect against herpes simplex Virus-2 in human vaginal epithelial cells by inhibiting the PI3K/Akt/mTOR pathway

Shao

Liu

Wang

et al. 2020

Journal of Ethnopharmacology

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“…In order to estimate the potential mechanism of TNF‐α and miR‐153 effect on MIN6 cells, AMPK/mTOR pathway was examined. AMPK/mTOR pathway is known as a common signaling pathway associated with tumors (An et al, ; C. Cao et al, ). It mainly takes part in the progression of proliferation and autophagy (An et al, ; Chang, Hsu, Leu, Chen, & Guh, ; H. Tang et al, ).…”

Section: Discussionmentioning

confidence: 99%

Retracted: Bilobalide alleviates tumor necrosis factor‐alpha‐induced pancreatic beta‐cell MIN6 apoptosis and dysfunction through upregulation of miR‐153

Hao

Wang

Wu³

et al. 2019

Phytotherapy Research

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Type 1 diabetes mellitus (T1DM) is a systemic disease and one classical type of total DM. Bilobalide (BB) is constituted of EGb 761. Our purpose was identifying the role of BB in TIDM in the current study. MIN6 cells were treated by TNF-α; then, viability, apoptosis, and insulin secretion were assessed by performing Cell Counting Kit-8 assay, flow cytometry, glucose-stimulated insulin secretion assay, and western blot.The effects of BB were assessed to identify its function. Further, the above mentioned parameters were reassessed when silencing miR-153. TNF-α declined viability and insulin secretion as well as raised apoptosis and inducible nitric oxide synthase (iNOS) expression in MIN6 cells. BB alleviated the apoptosis and dysfunction induced by TNF-α. MiR-153 expression was elevated by BB when induced by TNF-α. Increase of viability and insulin secretion as well as decline of apoptosis and iNOS induced by BB treatment was alleviated by silencing miR-153. The rates of p/t-p70S6K, p/tmammalian target of rapamycin (mTOR) and p/t-adenosine monophosphateactivated protein kinase (AMPK) were raised by BB and suppressed by silencing miR-153 under TNF-α induced condition. BB raised viability and insulin secretion, declined apoptosis and iNOS expression by up-regulating miR-153. Furthermore, BB activated AMPK/mTOR pathway by up-regulating miR-153.

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“…mTOR collects and transmits multiple autophagy signals upstream and negatively regulates the autophagy process. The activated mTOR complex mainly regulates the downstream target molecule through phosphorylation, p70S6K is one of its target molecules, and the p70S6K of phosphorylation is the marker of mTOR activation [48].…”

Section: Discussionmentioning

confidence: 99%

Roux-en-Y Gastric Bypass in Obese Diabetic Rats Promotes Autophagy to Improve Lipid Metabolism through mTOR/p70S6K Signaling Pathway

Tang

et al. 2020

Journal of Diabetes Research

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Purpose. To investigate the effects of Roux-en-Y gastric bypass (RYGB) surgery on markers of liver mitochondrial dynamics and find new therapeutic basis on obese type 2 diabetes mellitus (T2DM) patients. Materials and Methods. Thirty-two rats were divided into nondiabetic group, diabetic group, sham group, and RYGB group. The Dual-energy X-ray absorptiometry (DEXA) was used to detect short-term curriculum vitae for rat body component and fat and lean mass. Hepatic lipid content and triglyceride levels were detected by Oil Red O staining. Western blotting was used to examine autophagy and mammalian target of rapamycin/P70S6 kinase (mTOR/p70S6K) pathway-related proteins. The carbon dioxide production from the oxidation of [14C] oleate was measured. Plasma glucose was measured by glucose oxidase assay. The insulin and C-peptide were detected. Triacylglyceride (TG) and free fat acid (FFA) in plasma were determined by enzymatic colorimetric assays. Results. RYGB improved metabolic parameters and enhanced plasma GLP-1 level, ameliorated the lipopexia, and increased insulin sensitivity in the liver; RYGB promoted the hepatic autophagy and inhibited the mTOR/p70S6K signaling pathway. GLP-1 reduced fat load and increased fatty acid β-oxidation by activated autophagy to regulate the hepatic lipid pathway through mTOR/p70S6K signaling pathway. Conclusions. RYGB may reduce liver lipid toxicity and improve insulin sensitivity through activating the hepatic fat hydrolysis pathway and inhibiting the liver fat synthesis pathway. However, the transport pathway of liver fat does not play a key role.

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Ginkgo biloba exocarp extracts induces autophagy in Lewis lung cancer cells involving AMPK / mTOR / p70S6k signaling pathway

Cited by 27 publications

References 35 publications

The Chinese herbal prescription JZ-1 induces autophagy to protect against herpes simplex Virus-2 in human vaginal epithelial cells by inhibiting the PI3K/Akt/mTOR pathway

The Chinese herbal prescription JZ-1 induces autophagy to protect against herpes simplex Virus-2 in human vaginal epithelial cells by inhibiting the PI3K/Akt/mTOR pathway

Retracted: Bilobalide alleviates tumor necrosis factor‐alpha‐induced pancreatic beta‐cell MIN6 apoptosis and dysfunction through upregulation of miR‐153

Roux-en-Y Gastric Bypass in Obese Diabetic Rats Promotes Autophagy to Improve Lipid Metabolism through mTOR/p70S6K Signaling Pathway

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