Ginseng-derived exosome-like nanovesicles extracted by sucrose gradient ultracentrifugation to inhibit osteoclast differentiation

Seo, Kwansung; Yoo, Ji Hye; Kim, Jisu; Min, Sung Jun; Heo, Dong Nyoung; Kwon, Il Keun; Moon, Hojin

doi:10.1039/d2nr07018a

Cited by 39 publications

(11 citation statements)

References 37 publications

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“…In the majority of studies (61% or 17/28 studies), exosomes were isolated from humans; additionally, exosomes were isolated from mice in 6 studies (primarily RAW264.7 cells), rats in 4 studies (primarily rat BMSCs) and plant‐based exosomes in one study (ginseng). All target cells examined in vitro demonstrated the stimulatory effects of exosomes on osteogenic differentiation, with the exception of research conducted by Seo et al., 41 who investigated the impact of bone marrow‐derived macrophages on osteoclast inhibition by exosomes. The target tissue was BMSCs in 27/28 studies, of which 12 were performed on human BMSCs, whereas 7 studies were performed on rats and 9 were performed on mice.…”

Section: Resultsmentioning

confidence: 99%

Understanding exosomes: Part 3—therapeutic + diagnostic potential in dentistry

Miron,

Estrin,

Sculean

et al. 2024

Periodontology 2000

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Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with the ability to communicate with other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due to their ability to be utilized as therapeutic options for a wide array of various diseases. Over 5000 publications are currently being published on this topic yearly, many of which in the dental space. This extensive review article is the first scoping review aimed at summarizing all therapeutic uses of exosomes in regenerative dentistry. A total of 944 articles were identified as using exosomes in the dental field for either their regenerative/therapeutic potential or for diagnostic purposes derived from the oral cavity. In total, 113 research articles were selected for their regenerative potential (102 in vitro, 60 in vivo, 50 studies included both). Therapeutic exosomes were most commonly derived from dental pulps, periodontal ligament cells, gingival fibroblasts, stem cells from exfoliated deciduous teeth, and the apical papilla which have all been shown to facilitate the regenerative potential of a number of tissues including bone, cementum, the periodontal ligament, nerves, aid in orthodontic tooth movement, and relieve temporomandibular joint disorders, among others. Results demonstrate that the use of exosomes led to positive outcomes in 100% of studies. In the bone field, exosomes were found to perform equally as well or better than rhBMP2 while significantly reducing inflammation. Periodontitis animal models were treated with simple gingival injections of exosomes and benefits were even observed when the exosomes were administered intravenously. Exosomes are much more stable than growth factors and were shown to be far more resistant against degradation by periodontal pathogens found routinely in a periodontitis environment. Comparative studies in the field of periodontal regeneration found better outcomes for exosomes even when compared to their native parent stem cells. In total 47 diagnostic studies revealed a role for salivary/crevicular fluid exosomes for the diagnosis of birth defects, cardiovascular disease, diabetes, gingival recession detection, gingivitis, irritable bowel syndrome, neurodegenerative disease, oral lichen planus, oral squamous cell carcinoma, oropharyngeal cancer detection, orthodontic root resorption, pancreatic cancer, periodontitis, peri‐implantitis, Sjögren syndrome, and various systemic diseases. Hence, we characterize the exosomes as possessing “remarkable” potential, serving as a valuable tool for clinicians with significant advantages.

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Section: Resultsmentioning

confidence: 99%

Understanding exosomes: Part 3—therapeutic + diagnostic potential in dentistry

Miron,

Estrin,

Sculean

et al. 2024

Periodontology 2000

View full text Add to dashboard Cite

show abstract

“… 26 These methods involve extracting ELNs from plant tissues or cell cultures, followed by meticulous separation approaches such as differential centrifugation, ultrafiltration, or precipitation methods. 27 The choice of isolation method depends on factors such as desired yield, purity, and specific downstream applications, ensuring the procurement of well-defined plant ELN populations.…”

Section: Properties and Characteristics Of Plant Elnsmentioning

confidence: 99%

“…Bone tissue engineering has emerged as a promising approach for repairing and regenerating damaged or lost bone tissue, and plant ELNs offer unique properties that make them attractive for promoting bone regeneration. 27 The small size, biocompatibility, and cargo-carrying capabilities of plant ELNs contribute to their potential in this field.…”

Section: Application Of Plant Elns In Promotion Of Different Tissue R...mentioning

confidence: 99%

Plant-Derived Exosome-Like Nanoparticles: Emerging Nanosystems for Enhanced Tissue Engineering

Feng,

Yue,

Zhang

et al. 2024

IJN

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Tissue engineering holds great potential for tissue repair and rejuvenation. Plant-derived exosome-like nanoparticles (ELNs) have recently emerged as a promising avenue in tissue engineering. However, there is an urgent need to understand how plant ELNs can be therapeutically applied in clinical disease management, especially for tissue regeneration. In this review, we comprehensively examine the properties, characteristics, and isolation techniques of plant ELNs. We also discuss their impact on the immune system, compatibility with the human body, and their role in tissue regeneration. To ensure the suitability of plant ELNs for tissue engineering, we explore various engineering and modification strategies. Additionally, we provide insights into the progress of commercialization and industrial perspectives on plant ELNs. This review aims to highlight the potential of plant ELNs in regenerative medicine by exploring the current research landscape and key findings.

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“…EVs secreted by plants contain mRNAs, proteins, miRNAs, and bioactive lipids with unique and diverse pharmacological mechanisms that can exert multiple effect, such as antioxidant, anti-inflammatory, and antiosteoporotic effects. Studies have reported that EVs isolated from yams, 164 ginseng, 165 plums, 166 and apples. 167 have antiosteoporotic effects.…”

Section: The Potential Role Of Evs Derived From Different Sources In ...mentioning

confidence: 99%

The role and applications of extracellular vesicles in osteoporosis

Fang,

Yang,

Wang

et al. 2024

Bone Res

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Osteoporosis is a widely observed condition characterized by the systemic deterioration of bone mass and microarchitecture, which increases patient susceptibility to fragile fractures. The intricate mechanisms governing bone homeostasis are substantially impacted by extracellular vesicles (EVs), which play crucial roles in both pathological and physiological contexts. EVs derived from various sources exert distinct effects on osteoporosis. Specifically, EVs released by osteoblasts, endothelial cells, myocytes, and mesenchymal stem cells contribute to bone formation due to their unique cargo of proteins, miRNAs, and cytokines. Conversely, EVs secreted by osteoclasts and immune cells promote bone resorption and inhibit bone formation. Furthermore, the use of EVs as therapeutic modalities or biomaterials for diagnosing and managing osteoporosis is promising. Here, we review the current understanding of the impact of EVs on bone homeostasis, including the classification and biogenesis of EVs and the intricate regulatory mechanisms of EVs in osteoporosis. Furthermore, we present an overview of the latest research progress on diagnosing and treating osteoporosis by using EVs. Finally, we discuss the challenges and prospects of translational research on the use of EVs in osteoporosis.

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Ginseng-derived exosome-like nanovesicles extracted by sucrose gradient ultracentrifugation to inhibit osteoclast differentiation

Abstract: Ginseng-derived extracellular nanovesicles effectively blocked bone loss both in vitro and in vivo by inhibiting osteoclast differentiation through RANKL-induced IκBα, JNK, and ERK activation.

Cited by 39 publications

References 37 publications

Understanding exosomes: Part 3—therapeutic + diagnostic potential in dentistry

Understanding exosomes: Part 3—therapeutic + diagnostic potential in dentistry

Plant-Derived Exosome-Like Nanoparticles: Emerging Nanosystems for Enhanced Tissue Engineering

The role and applications of extracellular vesicles in osteoporosis

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