Ginseng polysaccharide attenuates red blood cells oxidative stress injury by regulating red blood cells glycolysis and liver gluconeogenesis

Wang, Siming; Zhao, Yushun; Yang, Junjie; Liu, Shichao; Ni, Weifeng; Bai, Xueyuan; Yang, Ze; Zhao, Daqing; Liu, Meichen

doi:10.1016/j.jep.2022.115716

Cited by 16 publications

(7 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Structural changes in RBCs’ membrane lipids, proteins, and hemoglobin (Hb) are known to be caused by excess H 2 O 2 , leading to oxidative hemolysis of these cells [ 22 ] and the release of Hb. Thus, measurement of Hb absorbance provides an indication of the degree of RBCs damage.…”

Section: Resultsmentioning

confidence: 99%

“…Damage experiments were carried out without enzymes; control experiments were performed without enzymes and H 2 O 2 ; toxicity control experiments were carried out only with fusion proteins. After treatment for 1 h at 37 °C, the samples were centrifuged at 2500 rpm for 10 min at 4 °C, and the absorbance was detected at 412 nm [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…The toxicity of fusion enzymes was evaluated during the interim period, and there were no significant differences in the hemolysis rates of the fusion enzymes compared to the control groups. Structural changes in RBCs' membrane lipids, proteins, and hemoglobin (Hb) are known to be caused by excess H 2 O 2 , leading to oxidative hemolysis of these cells [22] and the release of Hb. Thus, measurement of Hb absorbance provides an indication of the degree of RBCs damage.…”

Section: Se-hgpx1uag-l4-sod3-72p and Se-hgpx4uag-l3-sod3-72p Aid In P...mentioning

confidence: 99%

See 2 more Smart Citations

Study on the Properties and Synergistic Antioxidant Effects of Novel Bifunctional Fusion Proteins Expressed Using the UTuT6 System

Yan,

Wei,

Song

et al. 2023

Antioxidants

View full text Add to dashboard Cite

Important antioxidant enzymes, glutathione peroxidase (GPx) and superoxide dismutase (SOD), are involved in maintaining redox balance. They can protect each other and result in more efficiently removing excessive reactive oxygen species (ROS), protecting cells against injury, and maintaining the normal metabolism of ROS. In this study, human cytosolic GPx (hGPx1) and human phospholipid hydroperoxide GPx (hGPx4) genes were integrated into the same open reading frame with human extracellular SOD active site (SOD3-72P) genes, respectively, and several novel fusion proteins were obtained by using the UTuT6 expression system for the first time. Among them, Se-hGPx1UAG-L4-SOD3-72P is the bifunctional fusion protein with the highest GPx activity and the best anti-hydrogen peroxide inactivation ability thus far. The Se-hGPx4UAG-L3-SOD3-72P fusion protein exhibits the strongest alkali and high temperature resistance and a greater protective effect against lipoprotein peroxidation damage. Se-hGPx1UAG-L4-SOD3-72P and Se-hGPx4UAG-L3-SOD3-72P fusion proteins both have good synergistic and antioxidant abilities in H2O2-induced RBCs and liver damage models. We believe that this research will help with the development of novel bifunctional fusion proteins and the investigation of the synergistic and catalytic mechanisms of GPx and SOD, which are important in creating novel protein therapeutics.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Se-hgpx1uag-l4-sod3-72p and Se-hgpx4uag-l3-sod3-72p Aid In P...mentioning

confidence: 99%

See 1 more Smart Citation

Study on the Properties and Synergistic Antioxidant Effects of Novel Bifunctional Fusion Proteins Expressed Using the UTuT6 System

Yan,

Wei,

Song

et al. 2023

Antioxidants

View full text Add to dashboard Cite

show abstract

“…Due to these efforts, polysaccharides-which have distinct and particular properties-have been the preferred option in several applications. [63][64][65]…”

Section: Advantages and Disadvantages Of Polysaccharides In Sensors A...mentioning

confidence: 99%

Polysaccharides Based Biosensors for Medical Applications: Prospective and Future Aspects

2023

View full text Add to dashboard Cite

Biopolymers have attracted much of interest in various applications needed for sustainable and biodegradable solutions. Indeed, biopolymers lessen the requirement for petroleum‐based resources, municipal solid waste output, and carbon dioxide emissions. Polysaccharides are a unique category of biopolymers that are especially characteristic such as biocompatibility and editability. Polysaccharides functionalization plays a significant role in various applications and their suitability for additive applications. Sensor‐based polysaccharides are widely used with many drawbacks localized in durability, moldability, and sensitivity. Otherwise, biosensors are devices monitoring a specimen's biological phenomena or measurements, usually not disposable devices. For that, biosensors are needed urgently to address the high cost of disposable supplies in the medical diagnostic industry, which span many elements of medical diagnostic applications. Additionally, manipulating of polysaccharide‐based materials is considered accessible and usable and requires low equipment requirements. Herein the main features of polysaccharides will be discussed, along with potential applications as biosensors for medical diagnosis and new lines of inquiry with such focus on the main required to formulate polysaccharides biosensors.This article is protected by copyright. All rights reserved

show abstract

“…In addition, RG displayed antioxidant activity in mice. Experiments found that red ginseng polysaccharides (RGPS) significantly increased the levels of glutathione peroxidase (GSH-Px) and SOD and decreased the content of the free radical oxidation product MDA in serum and tissues to reduce the damage to the body [ 20 ]. There are also related studies showing that RG can accelerate burn wound healing in mice [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

By-Product of the Red Ginseng Manufacturing Process as Potential Material for Use as Cosmetics: Chemical Profiling and In Vitro Antioxidant and Whitening Activities

et al. 2022

View full text Add to dashboard Cite

Red ginseng (RG), which is obtained from heated Panax ginseng and is produced by steaming followed by drying, is a valuable herb in Asian countries. Steamed ginseng dew (SGD) is a by-product produced in processing red ginseng. In the present study, phytochemical profiling of extracts of red ginseng and steamed ginseng dew was carried out using gas chromatography-mass spectrometry (GC-MS) and rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) analysis. Additionally, antioxidant activities (DPPH, ·OH, and ABTS scavenging ability) and whitening activities (tyrosinase and elastase inhibitory activity) were analyzed. Phytochemical profiling revealed the presence of 66 and 28 compounds that were non-saponin components in chloroform extracts of red ginseng and steamed ginseng dew (RG-CE and SGD-CE), respectively. Meanwhile, there were 20 ginsenosides identified in n-butanol extracts of red ginseng and steamed ginseng dew (RG-NBE and SGD-NBE). By comparing the different polar extracts of red ginseng and steamed ginseng dew, it was found that the ethyl acetate extract of red ginseng (RG-EAE) had the best antioxidant capacity and whitening effect, the water extract of steamed ginseng dew (SGD-WE) had stronger antioxidant capacity, and the SGD-NBE and SGD-CE had a better whitening effect. This study shows that RG and SGD have tremendous potential to be used in the cosmetic industries.

show abstract

Ginseng polysaccharide attenuates red blood cells oxidative stress injury by regulating red blood cells glycolysis and liver gluconeogenesis

Cited by 16 publications

References 59 publications

Study on the Properties and Synergistic Antioxidant Effects of Novel Bifunctional Fusion Proteins Expressed Using the UTuT6 System

Study on the Properties and Synergistic Antioxidant Effects of Novel Bifunctional Fusion Proteins Expressed Using the UTuT6 System

Polysaccharides Based Biosensors for Medical Applications: Prospective and Future Aspects

By-Product of the Red Ginseng Manufacturing Process as Potential Material for Use as Cosmetics: Chemical Profiling and In Vitro Antioxidant and Whitening Activities

Contact Info

Product

Resources

About