Ginsenoside Re Promotes Nerve Regeneration by Facilitating the Proliferation, Differentiation and Migration of Schwann Cells via the ERK- and JNK-Dependent Pathway in Rat Model of Sciatic Nerve Crush Injury

Wang, Lei; Yuan, Damin; Zhang, Dongmei; Zhang, Weidong; Li, Chun; Cheng, Hongbing; Song, Yan; Tan, Qian

doi:10.1007/s10571-015-0177-7

Cited by 35 publications

(21 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…; Wang et al . ). This study showed increased expression of HMGB1 in nerve following injury and the source of this increase is infiltrating macrophages and proliferating Schwann cells.…”

Section: Discussionmentioning

confidence: 97%

Perineural expression of high‐mobility group box‐1 contributes to long‐lasting mechanical hypersensitivity via matrix metalloprotease‐9 up‐regulation in mice with painful peripheral neuropathy

Zhang

Morioka

Harano

et al. 2015

Journal of Neurochemistry

View full text Add to dashboard Cite

High-mobility group box-1 (HMGB1) has been shown to be critical in the modulation of nociceptive transduction following a peripheral neuropathy. However, the precise role of peripherally expressed HMGB1 in neuropathic pain has yet to be fully elaborated. Following a partial sciatic nerve ligation (PSNL) in mice, a persistent ipsilateral up-regulation of HMGB1 was observed from 3 to 21 days after PSNL, in paralleled with a robust ipsilateral hind paw mechanical hypersensitivity. Increased HMGB1 was detected in both infiltrating macrophages and proliferating Schwann cells in the ipsilateral nerve 14 days following PSNL. Repeated perineural treatment with anti-HMGB1 antibody significantly ameliorated PSNL-induced mechanical hypersensitivity. Several pronoci-ceptive molecules, including matrix metalloprotease-9 (MMP-9), tumor necrosis factor-a, interleukin-1b (IL-1b), and cyclooxygenase-2, were up-regulated in injured sciatic nerve 14 days following PSNL. Repeated perineural treatment with an anti-HMGB1 antibody significantly suppressed expression of MMP-9, but not other pronociceptive molecules. Perineural treatment with a selective MMP-9 inhibitor ameliorated PSNL-induced mechanical hypersensitivity. The current findings demonstrate that the maintenance of the neuropathic state following an injured nerve is dependent on the up-regulation of HMGB1 and MMP-9. Thus, blocking HMGB1 function in sciatic nerve could be a potent therapeutic strategy for the treatment of neuropathic pain. Abbreviations used: COX-2, cyclooxygenase-2; DRG, dorsal root ganglion; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HMGB1, high-mobility group box-1; IL-1b, interleukin-1b; IL-6, interleukin-6; iNOS, inducible nitric oxide; MBP, myelin basic protein; MMP-2, matrix metalloprotease-2; MMP-9, matrix metalloprotease-9; PSNL, partial sciatic nerve ligation; RAGE, receptor for advanced glycation end-products; SDS, sodium dodecyl sulfate; TIMP-1, tissue inhibitor of metalloproteinase 1; TLR, toll-like receptor; TNF-a, tumor necrosis factor-a.

show abstract

“…; Wang et al . ). This study showed increased expression of HMGB1 in nerve following injury and the source of this increase is infiltrating macrophages and proliferating Schwann cells.…”

Section: Discussionmentioning

confidence: 97%

Perineural expression of high‐mobility group box‐1 contributes to long‐lasting mechanical hypersensitivity via matrix metalloprotease‐9 up‐regulation in mice with painful peripheral neuropathy

Zhang

Morioka

Harano

et al. 2015

Journal of Neurochemistry

View full text Add to dashboard Cite

show abstract

“…Nerve regeneration process comprises a series of events from neuroinflammation and Schwann cells activation to expression of key genes and myelin remodeling, strongly implicated in NP development ( Wang et al, 2015 ). Our results showed that CFO treated mice presented a similar number of myelinated fibers to sham animals, opposed to the low count in vehicle treated animals.…”

Section: Discussionmentioning

confidence: 99%

Long-Chain Omega-3 Fatty Acids Supplementation Accelerates Nerve Regeneration and Prevents Neuropathic Pain Behavior in Mice

et al. 2017

View full text Add to dashboard Cite

Fish oil (FO) is the main source of long chain omega-3 polyunsaturated fatty acids (ω-3 PUFAs), which display relevant analgesic and anti-inflammatory properties. Peripheral nerve injury is driven by degeneration, neuroinflammation, and neuronal plasticity which results in neuropathic pain (NP) symptoms such as allodynia and hyperalgesia. We tested the preventive effect of an EPA/DHA-concentrate fish oil (CFO) on NP development and regenerative features. Swiss mice received daily oral treatment with CFO 4.6 or 2.3 g/kg for 10 days after NP was induced by partial sciatic nerve ligation. Mechanical allodynia and thermal hypernociception were assessed 5 days after injury. CFO 2.3 g/kg significantly prevented mechanical and thermal sensitization, reduced TNF levels in the spinal cord, sciatic MPO activity, and ATF-3 expression on DRG cells. CFO improved Sciatic Functional Index (SFI) as well as electrophysiological recordings, corroborating the increased GAP43 expression and total number of myelinated fibers observed in sciatic nerve. No locomotor activity impairment was observed in CFO treated groups. These results point to the regenerative and possibly protective properties of a combined EPA and DHA oral administration after peripheral nerve injury, as well as its anti-neuroinflammatory activity, evidencing ω-3 PUFAs promising therapeutic outcomes for NP treatment.

show abstract

“…As previous articles reported, our KEGG pathway analysis showed that cAMP signaling pathway, Wnt signaling pathway, ERK signaling pathway, PI3-Akt signaling pathway, Hippo/YAP signaling pathway, MAPK signaling pathway, and others were among the most relevant pathways for injured PNS repair process of SCs. Calcitonin gene-related peptides could activate the cAMP-PKA-ERK signaling cascade, which may play a direct role in initiating inflammatory processes in the PNS [28, 29]. Tao et al [30] discovered that carboxymethylated chitosan can promote the proliferation of cultured SCs and synthesis of nerve growth factor by activating the Wnt/b-catenin signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Comparison of DNA Methylation in Schwann Cells before and after Peripheral Nerve Injury in Rats

Zhou

Lin

Ren

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

This study aims to find the difference of genomewide DNA methylation in Schwann cells (SCs) before and after peripheral nerve system (PNS) injury by Methylated DNA Immunoprecipitation Sequencing (MeDIP-Seq) and seek meaningful differentially methylated genes related to repairment of injured PNS. SCs harvested from sciatic nerve were named as activated Schwann cells (ASCs), and the ones harvested from brachial plexus were named as normal Schwann cells (NSCs). Genomic DNA of ASCs and NSCs were isolated and MeDIP-Seq was conducted. Differentially methylated genes and regions were discovered and analyzed by bioinformatic methods. MeDIP-Seq analysis showed methylation differences were identified between ASCs and NSCs. The distribution of differentially methylated regions (DMRs) peaks in different components of genome was mainly located in distal intergenic regions. GO and KEGG analysis of these methylated genes were also conducted. The expression patterns of hypermethylated genes (Dgcr8, Zeb2, Dixdc1, Sox2, and Shh) and hypomethylated genes (Gpr126, Birc2) detected by qRT-PCR were opposite to the MeDIP analysis data with significance (p < 0.05), which proved MeDIP analysis data were real and believable. Our data serve as a basis for understanding the injury-induced epigenetic changes in SCs and the foundation for further studies on repair of PNS injury.

show abstract

Ginsenoside Re Promotes Nerve Regeneration by Facilitating the Proliferation, Differentiation and Migration of Schwann Cells via the ERK- and JNK-Dependent Pathway in Rat Model of Sciatic Nerve Crush Injury

Cited by 35 publications

References 55 publications

Perineural expression of high‐mobility group box‐1 contributes to long‐lasting mechanical hypersensitivity via matrix metalloprotease‐9 up‐regulation in mice with painful peripheral neuropathy

Perineural expression of high‐mobility group box‐1 contributes to long‐lasting mechanical hypersensitivity via matrix metalloprotease‐9 up‐regulation in mice with painful peripheral neuropathy

Long-Chain Omega-3 Fatty Acids Supplementation Accelerates Nerve Regeneration and Prevents Neuropathic Pain Behavior in Mice

Comparison of DNA Methylation in Schwann Cells before and after Peripheral Nerve Injury in Rats

Contact Info

Product

Resources

About