Ginsenoside Rg1 and platelet-rich fibrin enhance human breast adipose-derived stem cells function for soft tissue regeneration

Abstract

Adipose-derived stem cells (ASCs) can be used to repair soft tissue defects, wounds, burns, and scars and to regenerate various damaged tissues. The cell differentiation capacity of ASCs is crucial for engineered adipose tissue regeneration in reconstructive and plastic surgery. We previously reported that ginsenoside Rg1 (G-Rg1 or Rg1) promotes proliferation and differentiation of ASCs in vitro and in vivio. Here we show that both G-Rg1 and platelet-rich fibrin (PRF) improve the proliferation, differentiation, and soft tissue regeneration capacity of human breast adipose-derived stem cells (HBASCs) on collagen type I sponge scaffolds in vitro and in vivo. Three months after transplantation, tissue wet weight, adipocyte number, intracellular lipid, microvessel density, and gene and protein expression of VEGF, HIF-1α, and PPARγ were higher in both G-Rg1- and PRF-treated HBASCs than in control grafts. More extensive new adipose tissue formation was evident after treatment with G-Rg1 or PRF. In summary, G-Rg1 and/or PRF co-administration improves the function of HBASCs for soft tissue regeneration engineering.

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

“…Based on our previous studies, PRF is an excellent adjuvant for promoting the proliferation, differentiation, and paracrine function of ASCs [6]. In addition, functional optimization of NFSCs produced by PRF was also observed in this study (Figs.…”

“…The passaged cells grew markedly faster and reached the same confluence within 3 days compared with primary cells. All NFSC passages shared similar shapes and growth patterns with ASCs, which were obtained via the conventional collagenase digestion method described in our previous study [6]. After 2 weeks of inductive culture for adipocytes, osteoblasts, and chon-drocytes, positive staining for Oil Red O, alizarin red, and Alcian blue in cells indicated the multipotency of the NFSCs (Fig.…”

“…Multiple differentiation and paracrine functions of ASCs contribute to their tissue-repairing properties, which are improved by stimulation with platelet-rich fibrin (PRF) [6, 7]. PRF is also a known skin rejuvenator that has been used in esthetic medical procedures for anti-aging effects in skin [8].…”

Adipose derived stem cells and platelet rich plasma improve the tissue integration and angiogenesis of biodegradable scaffolds for soft tissue regeneration

“…The implants were implanted in the subcutaneous environment and thus the ADSCs may have either secreted required growth factors to generate adipose tissue or differentiated into adipose tissue to form soft tissue. To date, minimal evidence has demonstrated the in-situ capacity of ADSCs to form adipose tissue without differentiation medium as shown in this study [39]. Fang-Tian et al demonstrated that ADSCs with PRP and ginsenoside Rg1 on collagen type I scaffolds could support adipocyte differentiation in vivo after 3 months of implantation in a nude mouse model [39].…”

“…To date, minimal evidence has demonstrated the in-situ capacity of ADSCs to form adipose tissue without differentiation medium as shown in this study [39]. Fang-Tian et al demonstrated that ADSCs with PRP and ginsenoside Rg1 on collagen type I scaffolds could support adipocyte differentiation in vivo after 3 months of implantation in a nude mouse model [39]. However, further studies are needed to understand the potential of ADSCs to form adipose in vivo without the supporting signals of differentiation medium.…”