Ginsenoside Rg1 defenses PC-12 cells against hydrogen peroxide-caused damage via up-regulation of miR-216a-5p

Abstract

BackgroundSpinal cord injury (SCI) is a destructive trauma accompanying with local injury, half of which cause chronic paralysis. Ginsenoside Rg1 exerts anti-apoptosis and anti-autophagy properties. Therefore, our goal was to study the protective mechanism of Rg1 in attenuating cell injury.MethodsMiR-216a-5p inhibitor was transfected into PC-12 cells, then cells were pre-treated by Rg1 and treated with 300 μM hydrogen peroxide (H2O2) for 24 h. CCK-8 and apoptosis experiments were done to test cell activity and apoptosis respectively. Expression of miR-216a-5p and cell damage relative factors was tested via qRT-PCR and western blot experiments, respectively.ResultsH2O2 induced cell activity suppression, apoptosis and autophagy well at the concentration of 300 μM, leading cell injury. Rg1 could attenuate cell injury induced by H2O2 at the working concentration of 200 μM that it elevated cell activity, attenuated apoptosis and autophagy and activated PI3K/AKT and AMPK signal pathways. Further, miR-216a-5p was up-regulated by Rg1. Rg1 played its role in relieving cell injury by positively regulating miR-216a-5p.ConclusionOur study demonstrated that Rg1 attenuated H2O2-caused cell injury through positively regulated miR-216a-5p.

Chrysophanol protects PC12 cells against oxygen glucose deprivation-evoked injury by up-regulating miR-216a

“…More importantly, an investigation performed in primary cortical neuronal cells confirmed that elevating the expression of miR-216a could stimulate the neuroprotective effects on ischemic damages via targeting JAK2 both in vivo and in vitro [15]. Furthermore, a previous study specified that Ginsenoside Rg1, the primary active composition extracted from ginseng, exerted its protective roles against H 2 O 2 -triggered damages through elevating miR-216a-5p expression in PC12 cells [27]. Therefore, we suspected that CHR might exert its regulatory roles via mediating the expression of miR-216a as well.…”

Mechanisms of ginsenosides exert neuroprotective effects on spinal cord injury: A promising traditional Chinese medicine

“…To further clarify the mechanism, the PI3K inhibitor LY294002 was used to prove that the anti-apoptotic effect was achieved through the PI3K/Akt signaling pathway. Similarly, Rg1 exerts neuroprotection via the same pathway as Rb1 (Yi et al, 2019).…”