Ginsenoside Rg2 protects PC12 cells against β-amyloid25-35-induced apoptosis via the phosphoinositide 3-kinase/Akt pathway

Cui, Jing; Wang, Jiao; Zheng, Min; Gou, Dongxia; Liu, Chunming; Zhou, Yifa

doi:10.1016/j.cbi.2017.07.021

Cited by 53 publications

(31 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ginsenosides, known as saponins, are isolated from Asian ginseng ( Panax ginseng ). Ginsenoside has various pharmacological and therapeutic uses, such as antioxidative, antineoplastic, vasorelaxation, and antidiabetic and anti‐inflammatory effects (Chae et al., 2009; Cho et al., 2006; Cui et al., 2017; Leung & Wong, 2010; Mancuso & Santangelo, 2017; Wang et al., 2008). Ginsenoside Rb2 is a main active protopanaxadiol‐type compound in stem leaves and the root of ginseng (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rb2 alleviates myocardial ischemia/reperfusion injury in rats through SIRT1 activation

Xue

Liu

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

The cardioprotective effects of ginsenoside Rb2 on oxidative stress, which is induced by hydrogen peroxide and myocardial ischemia/reperfusion (MI/R) injury, have been studied. The mechanisms were associated with the inhibition of cardiomyocyte apoptosis, a high concentration of antioxidant defense enzymes, and scavenging oxidative stress products. Because of the association with oxidative reaction and cardioprotection, sirtuin‐1 (SIRT1) was selected as a promising target for investigating whether MI/R injury can be alleviated by ginsenoside Rb2 pretreatment through SIRT1 activation. The rats were exposed to ginsenoside Rb2 with or without SIRT1 inhibitor EX527 before ligation of coronary artery. Ginsenoside Rb2 reduced myocardial superoxide generation; downregulated gp91phox expression; and decreased the mRNA expression levels and activities of interleukin‐1β, interleukin‐6, and tumor necrosis factor‐α. The results demonstrated that ginsenoside Rb2 significantly attenuated oxidative stress and inflammation induced by MI/R injury. In addition, ginsenoside Rb2 upregulated SIRT1 expression and downregulated Ac‐p53 expression. However, EX527 blocked the protective effects, indicating that the pharmacological action of ginsenoside Rb2 involves SIRT1. Our results thus revealed that ginsenoside Rb2 alleviated MI/R injury in rats by inhibiting oxidative stress and inflammatory response through SIRT1 activation. Practical Application Ginsenoside Rb2 has a protective effect on MI/R injury by activating SIRT1 expression, reducing myocardium inflammation, and alleviating oxidative stress. Thus, ginsenoside Rb2 is a promising novel agent for ameliorating MI/R injury in ischemic heart diseases and cardiac surgery.

show abstract

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rb2 alleviates myocardial ischemia/reperfusion injury in rats through SIRT1 activation

Xue

Liu

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…Although AKT inhibitors have not been approved for the treatment of leukemia, there are several undergoing clinical trials and great promising potentials have emerged ( Lara et al, 2015 ). Saponins have been reported to inhibit the PI3K/AKT signaling pathway ( Cui et al, 2017 ). In the present study, TSPf suppressed the phosphorylation and activation of the AKT signaling as well as its downstream signals but had no effects on their total expression, suggesting that TSPf inhibits the activation of the AKT signaling.…”

Section: Discussionmentioning

confidence: 99%

Saponins From Paris forrestii (Takht.) H. Li Display Potent Activity Against Acute Myeloid Leukemia by Suppressing the RNF6/AKT/mTOR Signaling Pathway

Lü

Wang

et al. 2018

Front. Pharmacol.

View full text Add to dashboard Cite

Saponins are amphipathic glycosides found in traditional Chinese medicines. In the present study, we isolated a panel of saponins from Paris forrestii (Takht.) H. Li, a unique plant found in Tibet and Yunnan provinces, China. By examining their activities in suppressing acute myeloid leukemia (AML) cell proliferation, total saponins from Paris forrestii (TSPf) displayed more potent activity than individual ones. TSPf induced more than 40% AML cell apoptosis and decreased the viability of all leukemia cell lines. TSPf-induced apoptosis was confirmed by both Annexin V staining and caspase-3 activation. In line with these findings, TSPf downregulated pro-survival proteins Mcl-1, Bcl-xL, and Bcl-2 but upregulated the expression of tumor suppressor proteins p53, p27, Bax, and Beclin 1. The AKT/mTOR signaling pathway is frequently overactivated in various AML cells, and TSPf was found to suppress the activation of both AKT and mTOR, but had no effects on their total protein expression. This was further confirmed by the inactivation of 4EBP-1 and p70S6K, two typical downstream signal molecules in the AKT/mTOR pathway. Moreover, TSPf-inactivated AKT/mTOR signaling was found to be associated with downregulated RNF6, a recently identified oncogene in AML. RNF6 activated AKT/mTOR, and consistently, knockdown of RNF6 led to inactivation of the AKT/mTOR pathway. Furthermore, TSPf suppressed the growth of AML xenografts in nude mice models. Oral administration of TSPf almost fully suppressed tumor growth without gross toxicity. Consistent with the findings in cultured cell lines, TSPf also downregulated RNF6 expression along with inactivated AKT/mTOR signaling in tumor tissues. This study thus demonstrated that TSPf displays potent anti-AML activity by suppressing the RNF6/AKT/mTOR pathway. Given its low toxicity, TSPf could be developed for the treatment of AML.

show abstract

“…78 affecting the PI3K/Akt signalling pathway. 82 Moreover, numerous reports have shown that many other kinds of ginsenosides, such as Re, Rg1 and Rf, had the capability to downregulate the Aβ deposition and the tau protein phosphorylation. [83][84][85] These results suggest that these ginsenosides might have antidepressant effects through regulating Aβ.…”

Section: Effect On Other Mechanismsmentioning

confidence: 99%

Mechanisms of Panax ginseng action as an antidepressant

et al. 2019

View full text Add to dashboard Cite

Objectives Panax ginseng, a well‐known traditional Chinese medicine with multiple pharmacological activities, plays a crucial role in modulating mood disorders. Several recent studies have identified an underlying role of Panax ginseng in the prevention and treatment of depression. However, the cellular and molecular mechanisms remain unclear. Materials and Methods In this review, we summarized the recent progress of antidepressant effects and underlying mechanisms of Panax ginseng and its representative herbal formulae. Results The molecular and cellular mechanisms of Panax ginseng and its herbal formulae include modulating monoamine neurotransmitter system, upregulating the expression of neurotrophic factors, regulating the function of HPA axis, and anti‐inflammatory action. Conclusions Therefore, this review may provide theoretical bases and clinical applications for the treatment of depression by Panax ginseng and its representative herbal formulae.

show abstract

Ginsenoside Rg2 protects PC12 cells against β-amyloid25-35-induced apoptosis via the phosphoinositide 3-kinase/Akt pathway

Cited by 53 publications

References 31 publications

Ginsenoside Rb2 alleviates myocardial ischemia/reperfusion injury in rats through SIRT1 activation

Ginsenoside Rb2 alleviates myocardial ischemia/reperfusion injury in rats through SIRT1 activation

Saponins From Paris forrestii (Takht.) H. Li Display Potent Activity Against Acute Myeloid Leukemia by Suppressing the RNF6/AKT/mTOR Signaling Pathway

Mechanisms of Panax ginseng action as an antidepressant

Contact Info

Product

Resources

About