2022
DOI: 10.1101/2022.10.24.513352
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GiRAFR improves gRNA detection and annotation in single cell CRISPR screens

Abstract: Novel single cell RNA-seq analysis combined with CRISPR screens enables the high- throughput characterization of transcriptional changes caused by genetic perturbations. Dedicated software to annotate CRISPR guide RNA (gRNA) libraries and associate them with single cell transcriptomes are however lacking. Here, we generated a CRISPR droplet sequencing dataset. We demonstrate that the current default tool fails to detect mutant gRNAs. We therefore developed GiRAFR, a pysam-based software tool to characterize in… Show more

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“…GiRAFR is available as an open-source Python package at our GitHub Repository (github.com/FunctionalEpigeneticsLab/GiRAFR) and Zenodo repository with https://doi. org/10.5281/zenodo.8333711 24 . Scripts to recreate all figures using processed data are also included in this repository as jupyter notebooks.…”
Section: Data Availabilitymentioning
confidence: 99%
“…GiRAFR is available as an open-source Python package at our GitHub Repository (github.com/FunctionalEpigeneticsLab/GiRAFR) and Zenodo repository with https://doi. org/10.5281/zenodo.8333711 24 . Scripts to recreate all figures using processed data are also included in this repository as jupyter notebooks.…”
Section: Data Availabilitymentioning
confidence: 99%