GISH reveals different levels of meiotic pairing with wheat for individual Aegilops biuncialis chromosomes

Molnár, István; Molnár-Láng, Márta

doi:10.1007/s10535-010-0045-8

Cited by 11 publications

(9 citation statements)

References 38 publications

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“…biuncialis (MvGB642) addition lines 1U b , 1U b 6U b , 3U b , 2M b , 3M b and 7M b [12], and the substitution 3M b (4B) and the centric fusion 3M b .4BS, both obtained from a cross between Mv9kr1/ Ae. biuncialis (MvGB642) 3M b addition × Chinese Spring ph1b mutant [36], were produced in Martonvásár. The wheat (Chinese Spring)- Ae.…”

Section: Methodsmentioning

confidence: 99%

Syntenic Relationships between the U and M Genomes of Aegilops, Wheat and the Model Species Brachypodium and Rice as Revealed by COS Markers

et al. 2013

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Diploid Aegilops umbellulata and Ae. comosa and their natural allotetraploid hybrids Ae. biuncialis and Ae. geniculata are important wild gene sources for wheat. With the aim of assisting in alien gene transfer, this study provides gene-based conserved orthologous set (COS) markers for the U and M genome chromosomes. Out of the 140 markers tested on a series of wheat-Aegilops chromosome introgression lines and flow-sorted subgenomic chromosome fractions, 100 were assigned to Aegilops chromosomes and six and seven duplications were identified in the U and M genomes, respectively. The marker-specific EST sequences were BLAST-ed to Brachypodium and rice genomic sequences to investigate macrosyntenic relationships between the U and M genomes of Aegilops, wheat and the model species. Five syntenic regions of Brachypodium identified genome rearrangements differentiating the U genome from the M genome and from the D genome of wheat. All of them seem to have evolved at the diploid level and to have been modified differentially in the polyploid species Ae. biuncialis and Ae. geniculata. A certain level of wheat–Aegilops homology was detected for group 1, 2, 3 and 5 chromosomes, while a clearly rearranged structure was showed for the group 4, 6 and 7 Aegilops chromosomes relative to wheat. The conserved orthologous set markers assigned to Aegilops chromosomes promise to accelerate gene introgression by facilitating the identification of alien chromatin. The syntenic relationships between the Aegilops species, wheat and model species will facilitate the targeted development of new markers specific for U and M genomic regions and will contribute to the understanding of molecular processes related to allopolyploidization.

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Section: Methodsmentioning

confidence: 99%

Syntenic Relationships between the U and M Genomes of Aegilops, Wheat and the Model Species Brachypodium and Rice as Revealed by COS Markers

et al. 2013

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“…The Chinese Spring ph1b ( CSph1b ) mutant genotype (Sears 1977 ), which lacks the Ph1 locus, has been successfully used for the introgression of alien genetic material into the wheat genome by the induction of homoeologous pairing (Lukaszewski 2000 ). From this reason the intergenomic bivalent and trivalent appeared in Molnár and Molnár-Láng ( 2010 ) study. Considering presented study, FISH experiments showed that the pair of chromosomes 5B was present in all hybrids of each generation and probably is responsible for diploid-like pairing of chromosomes during meiosis, which was confirmed by ANOVA tests (Tables 4 , 5 and 6 ) that demonstrated no differences in means of chromosome configurations between hybrid plants.…”

Section: Discussionmentioning

confidence: 87%

“…variabilis × S. cereale ) × triticale ‘Lamberto.’ There were no intergenomic chromosome configurations observed in the plant carrying 2S v and/or 3S v chromosomes, which is opposite to other published studies concerning intergenomic hybridizations between cultivated cereals and Aegilops species. For example, Molnár and Molnár-Láng ( 2010 ) reported the intergenomic rod and ring bivalents and trivalent between 2 M, 3 M, 3U and 7 M chromosomes of Ae. biuncialis and wheat (Chinese Spring ph1b ) chromosomes.…”

Section: Discussionmentioning

confidence: 99%

Allocation of the S-genome chromosomes of Aegilops variabilis Eig. carrying powdery mildew resistance in triticale (× Triticosecale Wittmack)

et al. 2015

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It has been hypothesized that the powdery mildew adult plant resistance (APR) controlled by the Pm13 gene in Aegilops longissima Schweinf. & Muschl. (SlSl) has been evolutionary transferred to Aegilops variabilis Eig. (UUSS). The molecular marker analysis and the visual evaluation of powdery mildew symptoms in Ae. variabilis and the Ae. variabilis × Secale cereale amphiploid forms (2n = 6x = 42, UUSSRR) showed the presence of product that corresponded to Pm13 marker and the lower infection level compared to susceptible model, respectively. This study also describes the transfer of Ae. variabilis Eig. (2n = 4x = 28, UvUvSvSv) chromosomes, carrying powdery mildew resistance, into triticale (× Triticosecale Wittm., 2n = 6x = 42, AABBRR) using Ae. variabilis × S. cereale amphiploid forms. The individual chromosomes of Ae. variabilis, triticale ‘Lamberto’ and hybrids were characterized by genomic and fluorescence in situ hybridization (GISH/FISH). The chromosome configurations of obtained hybrid forms were studied at first metaphase of meiosis of pollen mother cells (PMCs) using GISH. The statistical analysis showed that the way of S-genome chromosome pairing and transmission to subsequent hybrid generations was diploid-like and had no influence on chromosome pairing of triticale chromosomes. The cytogenetic study of hybrid forms were supported by the marker-assisted selection using Pm13 marker and visual evaluation of natural infection by Blumeria graminis, that allowed to select the addition or substitution lines of hybrids carrying chromosome 3Sv which were tolerant to the powdery mildew infection.

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“…The Chinese Spring ph1b ( CSph1b ) mutant genotype (Sears 1977 ), which lacks the Ph1 locus, has been successfully used for the introgression of alien genetic material into the wheat genome by the induction of homoeologous pairing (Lukaszewski 2000 ). This is the reason for the intergenomic bivalent and trivalent appearance in the study of Molnár and Molnár-Láng ( 2010 ). In this work, the rDNA-FISH analysis and repetitive sequence FISH experiments showed that the pair of chromosomes 5B was present in all hybrids examined (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…According to the other studies concerning the meiotic analysis of Aegilops species with wheat and rye, the distant crosses should result in the intergenomic chromosome formation during meiosis. Molnár and Molnár-Láng ( 2010 ) observed the intergenomic rod and ring bivalents and trivalents between 2M, 3M, 3U, and 7M of Ae. biuncialis and wheat (Chinese Spring ph1b ) chromosomes.…”

Section: Resultsmentioning

confidence: 99%

Effective transfer of chromosomes carrying leaf rust resistance genes from Aegilops tauschii Coss. into hexaploid triticale (X Triticosecale Witt.) using Ae. tauschii × Secale cereale amphiploid forms

et al. 2014

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This paper shows the results of effective uses of a molecular cytogenetics toolbox and molecular marker to transfer leaf rust resistance genes from Aegilops tauschii × Secale cereale (DDRR, 2n = 4x = 28) amphiploid forms to triticale cv. Bogo (AABBRR, 2n = 6x = 42). The molecular markers of resistance genes and in situ hybridization analysis of mitotic metaphase of root meristems confirmed the stable inheritance of chromosome 3D segments carrying Lr32 from the BC2F2 to the BC2F5 generation of (Ae. tauschii × S. cereale) × triticale hybrids. The chromosome pairing analysis during metaphase I of meiosis of BC2F4 and BC2F5 hybrids showed increasing regular bivalent formation of 3D chromosome pairs and decreasing number of univalents in subsequent generations. The results indicate that using amphiploid forms as a bridge between wild and cultivated forms can be a successful technology to transfer the D-genome chromatin carrying leaf rust resistance genes into triticale.

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GISH reveals different levels of meiotic pairing with wheat for individual Aegilops biuncialis chromosomes

Cited by 11 publications

References 38 publications

Syntenic Relationships between the U and M Genomes of Aegilops, Wheat and the Model Species Brachypodium and Rice as Revealed by COS Markers

Syntenic Relationships between the U and M Genomes of Aegilops, Wheat and the Model Species Brachypodium and Rice as Revealed by COS Markers

Allocation of the S-genome chromosomes of Aegilops variabilis Eig. carrying powdery mildew resistance in triticale (× Triticosecale Wittmack)

Effective transfer of chromosomes carrying leaf rust resistance genes from Aegilops tauschii Coss. into hexaploid triticale (X Triticosecale Witt.) using Ae. tauschii × Secale cereale amphiploid forms

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