GJIC Enhances the phototoxicity of photofrin‐mediated photodynamic treatment by the mechanisms related with ROS and Calcium pathways

Wu, Dalin; Fan, Lixia; Xu, Chenyu; Liu, Zhen; Zhang, Yuan; Liu, Lucy; Wang, Qin; Tao, Liang

doi:10.1002/jbio.201400131

Cited by 14 publications

(24 citation statements)

References 44 publications

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“…Besides the above‐mentioned representative work, the research interests of China researchers are quite diverse, covering technique development through to biomedical applications. Consulting the papers published over the last three years in Journal of Biophotonics, the hot topics include photoacoustic microscopy , other technical improvement for imaging , photodynamic therapy , and human studies .…”

Section: Research Interests Indicated By Jbpmentioning

confidence: 99%

Biophotonics in China

Shi

Luo

2017

Journal of Biophotonics

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Biophotonics is a highly interdisciplinary field where physicists, chemists, biologists, physicians and engineers work together to solve the problems appearing in biology and medicine. In China, the Biophotonics discipline is often referred to as Biomedical Photonics, under the first‐level disciplines Biomedical Engineering or Optical Engineering, and was initiated in the late 1990s. Over the past 20 years, biophotonics research in China expanded extraordinarily and has reached the frontiers of the world‐level sciences. This white paper introduces the research groups in the biophotonics field in China, and their representative contributions.

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Section: Research Interests Indicated By Jbpmentioning

confidence: 99%

Biophotonics in China

Shi

Luo

2017

Journal of Biophotonics

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“…Photofrin‐mediated PDT has firstly been approved in 1993 in Canada for the prophylactic treatment of bladder cancer . Our previous studies showed that homotypic Cx32‐ and Cx26‐composed GJIC‐mediated “bystander effect” could enhance the phototoxicity of Photofrin‐mediated PDT . The present study represented the first demonstration of the ability of heterotypic Cx32/Cx26‐composed GJIC to have a “Good Samaritan” effect on HeLa cells following Photofrin‐PDT treatment.…”

Section: Introductionmentioning

confidence: 50%

“…The Western blot assay was utilized as described in our previous studies . Briefly, after protein samples were separated, samples were transferred to nitrocellulose transfer membrane (Excell Bio, China).…”

Section: Methodsmentioning

confidence: 99%

“…The assay was performed according to our previous studies for the measurement of gap junctional function . After donor and receiver cells were grown to 70–100% confluence, donor cells from one well were incubated with 5 µM calcein‐acetoxymethyl ester for 30 min, and then the cells were trypsinized and seeded to the receiver cells at 1:150 donor/receiver ratio for 4 hours at 37°C, and then donor cells were allowed to contact with the monolayer of receiver cells and form GJ.…”

Section: Methodsmentioning

confidence: 99%

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Decreased phototoxicity of photodynamic therapy by Cx32/Cx26‐composed GJIC: A “Good Samaritan” effect

Ding

Bai

et al. 2019

Lasers Surg Med

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Background and Objective Photodynamic therapy (PDT) has been widely used to treat malignant tumors. Our previous studies indicated that connexin (Cx) 32‐ and Cx26‐composed gap junctional intercellular communication (GJIC) could improve the phototoxicity of PDT. However, the role of heterotypic Cx32/Cx26‐formed GJIC in PDT phototoxicity is still unknown. Thus, the present study was aimed to investigate the effect of Cx32/Cx26‐formed GJIC on PDT efficacy. Methods CCK8 assay was used to detect cell survival after PDT. Western blot assay was utilized to detect Cx32/Cx26 expression. “Parachute” dye‐coupling assay was performed to measure the function of GJ channels. The intracellular Ca2+ concentrations were determined using flow cytometer. ELISA assay was performed to detect the intracellular levels of PGE2 and cAMP. Results The present study demonstrates there is a Cx32/Cx26‐formed GJIC‐dependent reduction of phototoxicity when cells were exposure to low concentration of Photofrin. Such a protective action is missing at low cell density due to the lack of GJ coupling. Under high‐cell density condition, where there is opportunity for the cells to contact each other and form GJ, suppressing Cx32/Cx26‐formed GJIC by either inhibiting the expression of Cx32/Cx26 or pretreating with GJ channel inhibitor augments PDT phototoxicity after cells were treated with at 2.5 µg/ml Photofrin. The above results suggest that at low Photofrin concentration, the presence of Cx32/Cx26‐formed GJIC may decrease the phototoxicity of PDT, leading to the insensitivity of malignant cells to PDT treatment. The GJIC‐mediated PDT insensitivity was associated with Ca2+ and prostaglandin E2 (PGE2) signaling pathways. Conclusion The present study provides a cautionary note that for tumors expressing Cx32/Cx26, the presence of Cx32/Cx26‐composed GJIC may cause the resistance of tumor cells to PDT. Oppositely, treatment strategies designed to downregulate the expression of Cx32/Cx26 or restrain the function of Cx32/Cx26‐mediated GJIC may increase the sensitivity of malignant cell to PDT. Lasers Surg. Med. 51:301–308, 2019. © 2019 Wiley Periodicals, Inc.

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“…It is also important to isolate PDT agents inside drug delivery vehicles because these highly sensitive species could become toxic with exposure to even ambient levels of light. 177 Nevertheless, due to the high level of similarity between PDT and PTT, it made sense to design systems with both modes of therapy integrated. Jang et al demonstrated an interesting example by using AuNRs as both carrier and quencher for the PDT agent, aluminum phthalocyanine tetrasulfonate (AlPcS 4 ).…”

Section: Combination Phototherapiesmentioning

confidence: 99%

Review of the progress toward achieving heat confinement—the holy grail of photothermal therapy

Sheng

Seare³

et al. 2017

J. Biomed. Opt

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Photothermal therapy (PTT) involves the application of normally benign light wavelengths in combination with efficient photothermal (PT) agents that convert the absorbed light to heat to ablate selected cancers. The major challenge in PTT is the ability to confine heating and thus direct cellular death to precisely where PT agents are located. The dominant strategy in the field has been to create large libraries of PT agents with increased absorption capabilities and to enhance their delivery and accumulation to achieve sufficiently high concentrations in the tissue targets of interest. While the challenge of material confinement is important for achieving “heat and lethality confinement,” this review article suggests another key prospective strategy to make this goal a reality. In this approach, equal emphasis is placed on selecting parameters of light exposure, including wavelength, duration, power density, and total power supplied, based on the intrinsic properties and geometry of tissue targets that influence heat dissipation, to truly achieve heat confinement. This review highlights significant milestones researchers have achieved, as well as examples that suggest future research directions, in this promising technique, as it becomes more relevant in clinical cancer therapy and other noncancer applications.

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GJIC Enhances the phototoxicity of photofrin‐mediated photodynamic treatment by the mechanisms related with ROS and Calcium pathways

Cited by 14 publications

References 44 publications

Biophotonics in China

Biophotonics in China

Decreased phototoxicity of photodynamic therapy by Cx32/Cx26‐composed GJIC: A “Good Samaritan” effect

Review of the progress toward achieving heat confinement—the holy grail of photothermal therapy

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