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Mishra, Arun Kumar

doi:10.1023/a:1025154822017

World Journal of Microbiology and Biotechnology

2003

DOI: 10.1023/a:1025154822017

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Arun Kumar Mishra

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2009

2024

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Cited by 4 publications

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Characterization of frankial strains isolated from Hippophae salicifolia D. Don, based on physiological, SDS–PAGE of whole cell proteins and RAPD PCR analyses

Singh

Srivastava

et al. 2009

World J Microbiol Biotechnol

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Different Frankia strains (HsIi2, HsIi4, HsIi5, HsIi8, HsIi9, HsIi10, HsIi11, HsIi12, HsIi13, HsIi14) nodulating Hippophae salicifolia D. Don, were characterized on the basis of physiological, biochemical and molecular attributes. Results suggest that the physiological approaches i.e., nitrogenase activity, glutamine synthetase (GS) activity and ammonia excretion are strain specific. The highest rate of nitrogen fixation and maximum production of ammonia with low GS makes the strain HsIi11, a suitable biofertilizer as compared to other strains. Analysis of total protein pattern (SDS-PAGE) revealed that the most closely related strains HsIi10 and HsIi4 were found to be most distantly related to the most similar strains HsIi14, HsIi5, HsIi13, HsIi11 and HsIi12. RAPD PCR analyses with an arbitrary primer 1253 produced distinct, unique and specific DNA fingerprints for each of the Frankia strain and 100% polymorphism was observed which uncovers the genetic diversity. These approaches might be helpful in rapid identification, in designing the marker for the specific strains as well as in improving nitrogen fixation in agroforestry.

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Characterization of frankial strains isolated from Hippophae salicifolia D. Don, based on physiological, SDS–PAGE of whole cell proteins and RAPD PCR analyses

Singh

Srivastava

et al. 2009

World J Microbiol Biotechnol

View full text Add to dashboard Cite

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Effect of salinity stress on growth, chlorophyll, antioxidant enzymes and nutrient content in Azolla spp.

Narayan,

Kumar,

Chowdhury

et al. 2024

Aquatic Botany

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Regulation of calcium ion and its effect on growth and developmental behavior in wild type and ntcA mutant of Anabaena sp. PCC 7120 under varied levels of CaCl2

Singh

Mishra

2014

Microbiology

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Untitled

Cited by 4 publications

References 30 publications

Characterization of frankial strains isolated from Hippophae salicifolia D. Don, based on physiological, SDS–PAGE of whole cell proteins and RAPD PCR analyses

Characterization of frankial strains isolated from Hippophae salicifolia D. Don, based on physiological, SDS–PAGE of whole cell proteins and RAPD PCR analyses

Effect of salinity stress on growth, chlorophyll, antioxidant enzymes and nutrient content in Azolla spp.

Regulation of calcium ion and its effect on growth and developmental behavior in wild type and ntcA mutant of Anabaena sp. PCC 7120 under varied levels of CaCl2

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