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Salvi, Neeta D.; Singh, Hemraj; Tivarekar, Suchita; Eapen, Susan

doi:10.1023/a:1010672809141

Cited by 24 publications

(9 citation statements)

References 5 publications

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“…IBA at lower and higher concentrations than 2.46 lM resulted in undesirable rooting response (Table 5). Though rooting was 100% in all the cases but in 1.23 lM IBA, delayed rooting with the induction of thin roots which could IBA has also been reported suitable for rooting in neem by several workers (Eeswara et al 1998;Salvi et al 2001;Quraishi et al 2004). However, requirement of higher concentrations of IBA is reported by them.…”

Section: Rooting Of Isolated Shootsmentioning

confidence: 76%

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

et al. 2009

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An efficient in vitro process for rapid clonal propagation of a 40-year-old tree of Azadirachta indica employing nodal stem segments was developed. Season of collection and maturity of explants showed direct influence on bud-break. Nodal stem segments collected during the month of April gave best response. Maximum bud-break (78.6-81%) was obtained when middle order nodes (3rd or 4th node from apex) were taken. Amongst different cytokinins used, 6-benzylaminopurine (BAP) at the concentration of 1.11 lM was found most effective in inducing multiple shoots, whereas inorganic and organic constituents of the medium influenced growth and general condition of proliferating shoots. On an average 3.1 shoots per explant were regenerated in modified Murashige and Skoog medium supplemented with 1.11 lM BAP, 1.43 lM indole-3-acetic acid (IAA) and 81.43 lM adenine hemisulphate. Isolated shoots were rooted in presence of 2.46 lM indole-3-butryic acid (IBA). Root induction took place in 8-10 days with 100% rooting. The in vitro-raised plantlets were successfully transplanted in potted soil and finally grown under field conditions with 100% survival. The genetic fidelity of such in vitro-raised field-grown plants was ascertained by random amplified polymorphic DNA (RAPD) markers. Furthermore, the azadirachtin content of in vitro-cloned plants was found comparable to the mother tree proving their chemical stability also. The protocol developed holds good for in vitro cloning of mature elite neem trees.

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Section: Rooting Of Isolated Shootsmentioning

confidence: 76%

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

et al. 2009

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“…In vitro propagation of neem has been reported via both direct (Ramesh and Padhya 1990;Joshi and Thengane 1996;Zypman et al 1997;Eeswara et al 1998) and indirect (Zypman et al 1997;Salvi et al 2001) morphogenetic pathways.…”

Section: Introductionmentioning

confidence: 99%

Effects of flask sealing and growth regulators on in vitro propagation of neem (Azadirachta indica A. Juss.)

Rodrigues

Costa

Festucci-Buselli

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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In vitro propagation of neem (Azadirachta indica A. Juss.) may offer an efficient alternative to seed propagation of this species. For optimization of in vitro propagation, different basal salt formulations, growth regulators, and culture container sealants (polytetrafluoroethylene hydrophobic membranes [PTFE]) were evaluated. Nodal segments cultured on Murashige and Skoog (MS) medium showed the highest shoot formation per explant (1.67). Explants cultured in flasks containing MS medium with 0.5 mg L −1 benzyladenine, 0.5 mg L −1 kinetin, and 0.05 mg L −1 naphthaleneacetic acid, and sealed with two PTFE membranes, produced the highest number of shoots (4.04). In contrast, explants cultured in flasks without membranes showed leaf chlorosis and senescence. For plant recovery, regenerants were acclimatized in a substrate of coconut fiber and eucalyptus bark (1:1) and showed 80% survival. Our results indicated that the use of flasks with vents was beneficial for in vitro propagation of this important plant.

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“…However, the number of regenerants has greatly reduced when BAP was supplemented with IAA, which gives a support to the hypothesis that high endogenous auxin status inhibits the organogenetic process. Regenerant differentiation from neem leaflets has also been reported by Narayan and Jaiswal (1985), Eeswara et al (1998) and Salvi et al (2001) but in most of the cases source of the explant is juvenile plants and/or regenerant differentiation involves intervening callus. Though, Ramesh and Padhya (1990) have employed leaflets from mature neem tree no mention about the age of the tree has been made.…”

Section: Resultsmentioning

confidence: 53%

Control of pattern of regenerant differentiation and plantlet production from leaflet segments of Azadirachta indica A. Juss. (neem)

Arora

Sharma

2008

Acta Physiol Plant

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A process with controlled pattern of regenerant differentiation from leaflet segments leading to production of cloned plants of a 40-year-old tree of Azadirachta indica was developed. A two-step procedure was adopted for containing intervening callusing during regenerant differentiation using modified Murashige and Skoog (MS) medium, where in the first step the explants were subjected to pulse treatments having higher concentration of 6-benzylaminopurine (BAP), while in the second step they were cultured in one-tenth of the initial concentrations of BAP. In the present case, simultaneous differentiation of two types of morphogenetic structures, that is, shoot buds and the meristematic nodules was observed. However, differentiation of higher number of desirable regenerants-the shoot buds and a few meristematic nodules, rather than vice-versa could be controlled by increasing both, the concentration of BAP in pulse treatment and the duration of pulse treatment. In the optimum treatment, where the explants were exposed to 8.88 lM BAP and 81.43 lM adenine hemisulphate for 5 days followed by their transfer to 0.88 lM BAP and 81.43 lM adenine hemisulphate, on an average, 17.4 shoot buds and only 1.6 meristematic nodules were formed from a leaflet. On subculturing, the shoot buds developed into shoots, whereas the meristematic nodules produced three kinds of organized structures that too in varied proportions. Multiplication of shoots was sustained in proliferation medium supplemented with 1.11 lM BAP, 1.43 lM indole-3-acetic acid (IAA) and 135.72 lM adenine hemisulphate. The isolated shoots were rooted and complete plantlets were transferred to potted soil with 100% survival.

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Cited by 24 publications

References 5 publications

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

Effects of flask sealing and growth regulators on in vitro propagation of neem (Azadirachta indica A. Juss.)

Control of pattern of regenerant differentiation and plantlet production from leaflet segments of Azadirachta indica A. Juss. (neem)

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