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Tovey, Euan R.; Taylor, David; Graham, Anthony H.; O'Meara, T.J.; Lovborg, U.; Jones, A.D.; Sporik, Richard

doi:10.1023/a:1007604430700

Cited by 17 publications

(3 citation statements)

References 4 publications

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“…The halogen immunoassay relies on the detection of airborne particles by capturing them onto protein-binding membranes by volumetric air sampling (Tovey et al 2000). Eluted antigens are either detected with monoclonal antibodies (mAbs) to identify specific antigens of interest (environmental monitoring) or immunostained with human serum IgE to identify the patient's sensitization patterns (serological monitoring) (Green, Millecchia, et al 2006).…”

Section: Enzyme-based Halogen Immunoassay (Hia)mentioning

confidence: 99%

Assays and enumeration of bioaerosols-traditional approaches to modern practices

King

Lacey

Pak

et al. 2020

Aerosol Science and Technology

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In recent years, much attention has been drawn to the exposure to bioaerosols in both occupational and indoor environments due to adverse effects on human health. Exposure to these agents may cause infectious diseases, allergic diseases, acute toxic effects, respiratory diseases, neurological effects, and cancer. Bioaerosols play a significant role in air quality studies, as well as in industrial and agricultural regulations. There is a diversity of sources for bioaerosol exposures, which include occupational activities such as waste disposal, sorting and composting, agricultural and food processing, livestock production and handling, healthcare, and fungal growth following flooding. Bioaerosol monitoring includes the measurement of viable (culturable and nonculturable) and nonviable microorganisms in both indoor and outdoor environments. The development of standardized methods for the detection and quantitation of bioaerosols has become an important issue. Technologies, including microbial plating, physical-chemical assays, and molecular techniques, have been adapted for the assessment of collected bioaerosol particles. In this review, we will discuss traditional and modern assays that have been applied in bioaerosol enumeration based on culturability, optical properties, immunoreactions, and nucleic acid amplification and sequencing of biological particles. The analysis of additional characteristics of bioaerosols including microbiome and antimicrobial resistance is also discussed. Finally, concluding thoughts are offered regarding the challenges and perspectives in the field.

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Section: Enzyme-based Halogen Immunoassay (Hia)mentioning

confidence: 99%

Assays and enumeration of bioaerosols-traditional approaches to modern practices

King

Lacey

Pak

et al. 2020

Aerosol Science and Technology

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“…The halogen immunoassay, initially developed by O’Meara et al [36] to detect inhaled cat allergen, described in detail by Tovey et al [37], and later refined for fungal allergens by Mitakakis et al [38] and Green et al [19,20,21,35,39,40] is an immunoblotting method with some similarities to the enzyme-link immunosorbent assay and Western blot techniques. Advantages of this technique are that it is not dependent on any extract and the particle to be tested is induced to elute allergen.…”

Section: Introductionmentioning

confidence: 99%

Sensitization to Airborne Ascospores, Basidiospores, and Fungal Fragments in Allergic Rhinitis and Asthmatic Subjects in San Juan, Puerto Rico

Rivera-Mariani

Nazario-Jiménez²,

López-Malpica³

et al. 2011

Int Arch Allergy Immunol

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Background: Fungal spores are the predominant biological particulate in the atmosphere of Puerto Rico, yet their potential as allergens has not been studied in subjects with respiratory allergies. The purpose of this study was to determine the level of sensitization of subjects with respiratory allergies to these particles. Methods: Serum samples were drawn from 33 subjects with asthma, allergic rhinitis, or nonallergic rhinitis and 2 controls with different skin prick test reactivity. An MK-3 sampler was used to collect air samples and the reactivity of the sera to fungal particles was detected with a halogen immunoassay. Results: All subjects reacted to at least 1 fungal particle. Thirty-one subjects reacted to ascospores, 29 to basidiospores, 19 to hyphae/fungal fragments, and 12 to mitospores. The median percentage of haloes in allergic rhinitis subjects was 4.82% while asthma or nonallergic rhinitis subjects had values of 1.09 and 0.39%, respectively. Subjects with skin prick tests positive to 3, 2, 1, or no extract had 5.24, 1.09, 1.61, and, 0.57% of haloed particles, respectively. If skin prick tests were positive to basidiomycetes, pollen, animals, or deuteromycetes, the percentages of haloes were 4.72, 4.15, 3.63, and 3.31%, respectively. Of all haloed particles, 46% were unidentified, 25% ascospores, 20% basidiospores, 7% hyphae/fungal fragments, and 2% mitospores. IgE levels and the number of positive skin prick test extracts correlated with the percentage of haloes. Conclusion: In tropical environments, sensitization to airborne basidiomycetes, ascomycetes, and fungal fragments seems to be more prevalent than sensitization to mitospores in subjects with active allergies, suggesting a possible role in exacerbations of respiratory allergies.

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“…and Cladiosporium spp.) have been associated with allergenic reactions (TOVEY et al 2000). Airborne fungal spores are the main mechanism of dispersal of many plant pathogenic and saprophytic fungi and thus are found in a very wide range of environments.…”

Section: Introductionmentioning

confidence: 99%

New methods for detecting and enumerating fungal spores of plant pathogens

Kennedy¹,

Wakeham²

2002

Plant Prot. Sci.

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Information on the presence or absence of airborne spores or other particles would be useful in an increasing number of areas including agriculture. Traditional methods used for detecting and enumerating of airborne spores of fungal plant pathogens are time consuming and require specialist knowledge. Some spore types (e.g. ascospores) are difficult to differentiate using these methods. To facilitate this, new methods, which can be used to accurately differentiate fungal spore types, are required. A Burkard 7-day volumetric spore trap used in combination with an immunofluorescence test has been developed to detect and quantify field-trapped ascosporic inoculum of Mycosphaerella brassicicola (the ringspot pathogen of brassicas). This test has also been found useful in the validation of more rapid user-friendly immunoassay based trapping procedures. A microtiter immunospore trapping device, which uses a suction system to directly trap airparticulates by impaction into microtiter wells, has been used successfully for the rapid detection and quantification of ascosporic inoculum of M. brassicicola. The system shows potential for the rapid field-detection of airborne ascosporic inoculum of the ringspot pathogen.

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Cited by 17 publications

References 4 publications

Assays and enumeration of bioaerosols-traditional approaches to modern practices

Assays and enumeration of bioaerosols-traditional approaches to modern practices

Sensitization to Airborne Ascospores, Basidiospores, and Fungal Fragments in Allergic Rhinitis and Asthmatic Subjects in San Juan, Puerto Rico

New methods for detecting and enumerating fungal spores of plant pathogens

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