Earth's climate has undergone dramatic shifts between glacial and interglacial time periods, with high-latitude temperature changes on the order of 5-10 • C. These climatic shifts have been associated with major rearrangements in the deep ocean circulation and stratification, which have likely played an important role in the observed atmospheric carbon dioxide swings by affecting the partitioning of carbon between the atmosphere and the ocean. The mechanisms by which the deep ocean circulation changed, however, are still unclear and represent a major challenge to our understanding of glacial climates. This study shows that various inferred changes in the deep ocean circulation and stratification between glacial and interglacial climates can be interpreted as a direct consequence of atmospheric temperature differences. Colder atmospheric temperatures lead to increased sea ice cover and formation rate around Antarctica. The associated enhanced brine rejection leads to a strongly increased deep ocean stratification, consistent with high abyssal salinities inferred for the last glacial maximum. The increased stratification goes together with a weakening and shoaling of the interhemispheric overturning circulation, again consistent with proxy evidence for the last glacial. The shallower interhemispheric overturning circulation makes room for slowly moving water of Antarctic origin, which explains the observed middepth radiocarbon age maximum and may play an important role in ocean carbon storage.LGM | AMOC | stratification | cooling | sea-ice T he deep ocean today is ventilated mainly by two water masses. North Atlantic deep water (NADW) is formed in the North Atlantic before flowing southward at a depth of about 2-3 km and eventually rising back up to the surface in the Southern Ocean. Antarctic bottom water (AABW) is formed around Antarctica and spreads northward into the abyssal basins. The AABW that makes it into the Atlantic then slowly upwells into the lower NADW before returning southward and resurfacing again around Antarctica (1, 2). The glacial equivalent of NADW was likely confined to shallower depths, leaving more of the deep Atlantic filled with water masses originating primarily from around Antarctica (3-5). Moreover, the two water masses appear more distinct, with less mixing between them (6).Multiple studies have pointed toward the potential importance of sea ice and surface buoyancy fluxes around Antarctica in controlling changes in the deep ocean stratification and circulation between the present and Last Glacial Maximum (LGM) (7-11). Specifically, we recently showed that enhanced buoyancy loss around Antarctica is expected to lead to an increase in the abyssal stratification, an upward shift of NADW, and a clearer separation between NADW and southward-flowing AABW (11)-all in agreement with inferences made for differences in circulation and stratification between the present and LGM (3,6,12,13). This gives rise to the hypothesis that strong cooling around Antarctica led to an increased net freezing...