Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats

Bowman, JP; McCammon, Sharee A.; Brown, Janelle; McMeekin, TA

doi:10.1099/00207713-48-4-1213

Cited by 105 publications

(116 citation statements)

References 20 publications

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“…These values are consistent with those of recognized members of the genus Glaciecola, which range between 40 and 46 mol% (Bowman et al, 1998).…”

supporting

confidence: 77%

“…Phenotypic characteristics of strains S18K6 T and S18K5 and of recognized Glaciecola species Taxa: 1, strain S18K6 T ; 2, strain S18K5; 3, Glaciecola mesophila; 4, Glaciecola polaris; 5, Glaciecola punicea; 6, Glaciecola pallidula. Data are taken from Bowman et al (1998), Romanenko et al (2003), Van Trappen et al (2004) and this study. Symbols: +, positive; 2, negative; V+, variable between strains, type strain positive; V2, variable between strains, type strain negative; W, weak or delayed; ND, not determined.…”

mentioning

confidence: 99%

“…We describe here two polysaccharideproducing bacterial strains that were considered to belong to the genus Glaciecola. The genus Glaciecola is a member of the Gammaproteobacteria (Bowman et al, 1998). The physiological and biochemical features, chemotaxonomic characteristics and phylogeny of the two strains, designated S18K6 T and S18K5, were examined.…”

mentioning

confidence: 99%

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Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium

Matsuyama

Hirabayashi

Kasahara

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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Two novel exopolysaccharide-producing bacteria, strains S18K6 T and S18K5, were isolated from Pacific Ocean sediment. The isolates were Gram-negative, motile, strictly aerobic chemoheterotrophic bacteria. The DNA G+C contents of strains S18K6 T and S18K5 were 44?8 and 46?3 mol%, respectively. DNA-DNA relatedness between the two strains was 70 %. Major fatty acids were hexadecanoic acid (C 16 : 0 ), hexadecenoic acid (C 16 : 1 v7c) and octadecenoic acid (C 18 : 1 v7c). 16S rRNA gene sequence, chemotaxonomic and morphological data indicated that these strains clearly belonged to the genus Glaciecola. Based on phenotypic properties and DNA-DNA hybridization data, strains S18K6 T and S18K5 are considered to represent a novel species of the genus Glaciecola, for which the name Glaciecola chathamensis sp. nov. is proposed. The type strain is S18K6 T (=JCM 13645 T =NCIMB 14146 T ).There have been many reports of micro-organisms that produce exopolysaccharides (EPSs). Some bacterial polysaccharides are produced on an industrial scale and are used as raw materials in food processing and in medical and industrial preparations. It remains possible that new polysaccharide-producing bacteria will be found in various habitats. In attempts to find new polysaccharide-producing bacteria from ocean bottom sediment we have successfully isolated numerous bacteria that produced polysaccharide in our laboratory. We describe here two polysaccharideproducing bacterial strains that were considered to belong to the genus Glaciecola. The genus Glaciecola is a member of the Gammaproteobacteria (Bowman et al., 1998). The physiological and biochemical features, chemotaxonomic characteristics and phylogeny of the two strains, designated S18K6 T and S18K5, were examined. DNA-DNA relatedness data showed that the strains should be classified as a novel species of the genus Glaciecola.Sediment samples were collected during the R/V HakuhoMaru cruise (KH-05-2) in 2005. Strains S18K6 T and S18K5 were isolated by selective enrichment from a sediment sample taken from the Pacific Ocean floor near Chatham Rise at a water depth of 4627 m (39 u 599 570 S 169 u 599 850 W). The sediment sample was inoculated in 10 ml mineral salts medium containing (per litre) 5 g peptone, 10 g glucose, 0?008 g Na 2 HPO 4 , 0?0016 g NH 4 NO 3 , 23?4 g NaCl, 0?7 g KCl, 10?6 g MgCl 2 .6H 2 O, 1?1 g CaCl 2 , 3?9 g Na 2 SO 4 , 0?2 g NaHCO 3 , 1?0 g (NH 4 ) 2 SO 4 , 0?01 g K 2 HPO 4 and 6?05 g Tris, pH 7?8. This medium was incubated at 15 u C with shaking at 150 r.p.m. After being incubated for several days, a portion of the suspension was transferred into 10 ml fresh medium and the medium was then reincubated. After three successive transfers, the suspension was plated onto solid medium to isolate pure cultures. The isolates were checked for their ability to produce EPSs by adding ethanol to the broth supernatant. Of the strains isolated, S18K6 T and S18K5, which showed good EPS production, were selected for further study. To investigate their morphological and physiological c...

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“…These values are consistent with those of recognized members of the genus Glaciecola, which range between 40 and 46 mol% (Bowman et al, 1998).…”

supporting

confidence: 77%

mentioning

confidence: 99%

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Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium

Matsuyama

Hirabayashi

Kasahara

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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“…Furthermore, strain X13M-12 T could be distinguished from the type strains of the type species of the genera Aestuariibacter, Salinimonas, Agaribacter, Aliiglaciecola, Glaciecola, Paraglaciecola and Alteromonas by the presence of C 17 : 0 cyclo and C 19 : 0 10-methyl, and due to the lack of C 15 : 0 . (Teramoto & Nishijima, 2014); 6, Aliiglaciecola (Chen et al, 2009a;Jean et al, 2013;Jin et al, 2015;Tanaka et al, 2010); 7, Glaciecola (Baik et al, 2006;Bowman et al, 1998); 8, 'Pseudobowmanella' (Du et al, 2015); 9, Paraglaciecola (Matsuyama et al, 2006;Park & Yoon, 2013;Romanenko et al, 2003;Shivaji & Reddy, 2014;Van Trappen et al, 2004a;Yong et al, 2007;Zhang et al, 2006;Zhang et al, 2011); 10, Alteromonas (Baumann et al, 1972;Chen et al, 2009b;Chiu et al, 2007;Ivanova et al, 2005;Ivanova et al, 2013;Ivanova et al, 2015;Martínez-Checa et al, 2005;Matsuyama et al, 2015;Van Trappen et al, 2004b;Vandecandelaere et al, 2008;Yi et al, 2004;Yoon et al, 2003;Yoon et al, 2004) Oxygen requirements…”

mentioning

confidence: 99%

“…The family Alteromonadaceae, belonging to the class Gammaproteobacteria, was first established by Ivanova & Mikhailov (2001) and consists of 18 genera with validly published names at the time of writing, including Aestuariibacter (Yi et al, 2004), Agaribacter (Teramoto & Nishijima, 2014), Agarivorans (Kurahashi & Yokota, 2004), Aliagarivorans (Jean et al, 2009), Aliiglaciecola (Jean et al, 2013), Alishewanella (Vogel et al, 2000), Alteromonas (Baumann et al, 1972), Bowmanella (Jean et al, 2006), Glaciecola (Bowman et al, 1998), Haliea (Urios et al, 2008), Marinimicrobium (Lim et al, 2006), Marinobacter (Gauthier et al, 1992), Marinobacterium (González et al, 1997), Microbulbifer (González et al, 1997), Paraglaciecola (Shivaji & Reddy, 2014), 'Pseudobowmanella' (Du et al, 2015), Saccharophagus (Ekborg et al, 2005) and Salinimonas (Jeon et al, 2005). Most members of the family Alteromonadaceae are characterized as being Gram-staining-negative, rod-shaped, motile and contain Q-8 as the major respiratory quinone (Ló pez-Pérez & Rodriguez-Valera, 2014).…”

mentioning

confidence: 99%

Lacimicrobium alkaliphilum gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from a salt lake

Zhong

Liu

Wang

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Lacimicrobium alkaliphilum gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from a salt lake A Gram-stain-negative, facultatively aerobic bacterium, strain X13M-12 T , was isolated from a salt lake (Lake Xiaochaidan) in the Qaidam basin, Qinghai Province, PR China. Cells of strain X13M-12 T were slightly curved, rod-shaped, 0.5-0.8 mm wide and 1.2-2.3 mm long, and motile by means of a polar flagellum. Strain X13M-12 T was catalase-and oxidase-positive. Growth was observed in the presence of 0-15.0 % (w/v) NaCl (optimum 3.0-5.0 %), and at 4-40 8C (optimum 25-30 8C) and pH 6.0-11.0 (optimum pH 8.5). Strain X13M-12 T contained Q-8 as the sole respiratory quinone, and phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The major cellular fatty acids (.10 % of totals) were C 16 : 0 , C 16 : 1 v7c and/or C 16 : 1 v6c, and C 18 : 1 v7c and/or C 18 : 1 v6c. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain X13M-12 T belonged to the family Alteromonadaceae and formed a distinct lineage, showing low gene sequence similarities to closely related genera: Bowmanella, Aestuariibacter and Salinimonas (16S rRNA gene sequence similarities, 93.0-93.1 %, 92.3-93.1 % and 92.6-92.7 %, respectively). In addition, strain X13M-12 T showed ,92.7 % gene sequence similarities to other species of the family Alteromonadaceae. The DNA G+C content of strain X13M-12 T was 49 mol% (Tm). Based on the data presented above, strain X13M-12 T is considered to represent a novel genus and species of the family Alteromonadaceae, for which the name Lacimicrobium alkaliphilum gen. nov., sp. nov. is proposed. The type strain is

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Psychrophilic Bacteria: Isolation and Characterization

Bowman

2003

Encyclopedia of Environmental Microbiology

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Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats

Cited by 105 publications

References 20 publications

Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium

Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium

Lacimicrobium alkaliphilum gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from a salt lake

Psychrophilic Bacteria: Isolation and Characterization

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