Cryoconite granules are mixtures of mineral particles, organic substances and organisms on the surface of glaciers where they decrease the ice albedo and are responsible for formation of water-filled holes. The contaminants are effectively trapped in the cryoconite granules and stay there for many years. This study evaluates the contamination level of artificial and natural radionuclides in cryoconite holes from Adishi glacier (Georgia) and identifies the sources of contamination based on activity or mass ratios among artificial radionuclides. Results revealed high activity concentrations of fallout radionuclides reaching 4900 Bq/kg, 2.5 Bq/kg, 107 Bq/kg and 68 Bq/kg for 137Cs, 238Pu, 239+240Pu and 241Am, respectively. The main source of Pu is global fallout, but the low 240Pu/239Pu atomic ratios also indicated local tropospheric source of 239Pu, probably from the Kapustin Yar nuclear test site. Also, high activity ratios of 241Am/239+240Pu could originate from Kapustin Yar. The natural radionuclides originate from the surrounding rocks and were measured to control the environmental processes. 210Pb in cryoconite granules comes predominantly from the atmospheric deposition, and its activity concentrations reach high values up to 12000 Bq/kg.