This paper examines the environmental impact of incorporating wood into glamping structures, revealing a significant net carbon sequestration effect. The wood used in these structures, sequestering 36.83 metric tons of CO2, surpasses the carbon emissions associated with the entire glamping life cycle, totaling 9.97 or 11.72 metric tons of carbon. One wood-based structure can store about 25 metric tons of carbon for 50 years. This noteworthy finding highlights how sustainably harvested wood can operate as a carbon sink, balancing carbon emissions produced throughout raw material procurement, manufacture, building, and use. The paper evaluates each step of manufacturing by life cycle assessment, applying the ‘cradle to grave’ approach. The assessment aims to identify possibilities for CO2 emission reduction in the supply value chain, developed horizontally in a region as well as vertically for glamping production among 7 Romanian companies. Such carbon-offsetting attributes offer a valuable tool in the fight against climate change, aligning with global sustainability goals. To maximize these benefits, sustainable sourcing and management of wood resources are essential, as is the adoption of carbon-positive design principles that prioritize carbon storage. Beyond carbon neutrality, this scenario presents an opportunity to educate stakeholders and guests about the significance of sustainable construction in mitigating climate change and the importance of material choices with low carbon footprints, carbon neutrality and environmental stewardship. The results of the study are presented in the context of other papers that have been written and published internationally, sharing the lessons learned from production and resource management techniques, with the aim of CO2 neutrality production from forest-based industry for tourism on the horizon.