Information needs for understanding cell divisions in the human body is important in the learning process. Although sketches, images and blocks of 3D puzzles were used for teaching and learning, unfortunately those tools are static and incapable of being manipulated. Hence, digital information is the best tool for the teaching and learning of cell divisions in the human body via software applications. A cell motion is a digital information application developed using leap motion to demonstrate cell movement in the human body. However, the factors that influence students towards adopting this application are not obvious and often ignored. The method for evaluating the factors influencing its user’s acceptance is the Technology Acceptance Model (TAM) via a questionnaire distributed among medical students to gain statistically valid quantitative results through hypothesis-testing. The result indicates that digital information needs for the understanding of cell divisions in the human body are influenced by the user’s Perceived Ease of Use (PEOU) and Perceived Usefulness (PU). However, the Attitude (AT) towards use did have a significant effect on PU and PEOU. Moreover, PEOU had a strong and significant influence on PU, while AT positively influenced users’ behavioural intention (BI) of using digital information needs for the understanding of cell divisions in the human body.