GLB‐13 is associated with oxidative stress resistance in caenorhabditis elegans

Abstract

Globins constitute a superfamily of heme-binding proteins that is widely present in many species. There are 33 putative globins in the genome of Caenorhabditis elegans, where glb-13 is a homolog of neuroglobin (Ngb) based on sequence analysis and specific expression in neurons. Here we examined whether glb-13 as well as Ngb is also associated with resistance to reactive oxygen species (ROS) induced by paraquat. Our results showed that the mRNA level of glb-13 was significantly upregulated after paraquat exposure. Expression of a green fluorescent protein (GFP) reporter gene directed by the glb-13 promoter was increased by paraquat exposure. The mutant C. elegans strain glb-13(tm2825) was sensitive to paraquat-induced oxidative stress. Overexpression of human Ngb (hNgb) in C. elegans neuronal cells can rescue the paraquat sensitive phenotype of the mutant strain. glb-13 mutation or hNgb overexpression did not affect the expression of antioxidant enzymes such as superoxide dismutase (SOD). To examine the ROS-scavenging capabilities of hNgb and glb-13, we further examined the level of ROS in glb-13 mutant and hNgb transgenic (hNgb-Tg) worms. There was no statistical difference in ROS levels in the untreated controls; however in paraquattreated worms, the ROS level was statistically repressed in the hNgb-Tg relative to enhanced green fluorescent protein (EGFP)-Tg worms or wildtype animals. Additionally, the ROS level of glb-13 mutant was statistically higher than the wildtype animals. Furthermore, hNgb overexpression diminished the ROS level of glb-13 mutant. In conclusion, hNgb can rescue the ROS sensitive phenotype of the glb-13 mutant strain. The protein GLB-13 seems to have an hNgb-like function, suggesting the importance of the globin protein family in maintaining the homeostasis of ROS signals. Our data provided evidence for the first time that glb-13 is associated with the resistance against oxidative stress-induced toxicity.

Limb remote ischemic per-conditioning in combination with post-conditioning reduces brain damage and promotes neuroglobin expression in the rat brain after ischemic stroke

“…neuronal cells from hypoxia/ischemia, ROS/NOS, or β -amyloid peptides ( Jin, et al., 2008 ; Liu, et al., 2009 ; Ren, et al., 2013 ). Consistently, our data show that Ngb is significantly increased at 7 and 14 days after combined remote ischemic conditioning, compared with ischemic control group suggesting that Ngb may be involved in PerC+PostC-mediated neuroprotection after focal ischemia.…”

“…Production of ROS was assayed using the ROS Fluorescent Determination Kit (GenMed Scientifics Inc., Arlington, MA, USA) ( Ren, et al., 2013 ). At day 3 after MCAO, samples were prepared according to manufacturer’s instruction.…”

Neuroglobin: From structure to function in health and disease

“…elegans shows 33 globins, diverse in gene and protein structure being localized mostly in neurons (Tilleman et al, 2011). Interestingly, globin-5 and globin-13 have been reported to be homologous to Ngb (Abergel et al, 2016;Kodama-Namba et al, 2013;Ren et al, 2013). Globin-5 is involved in the cross-modulation of homeostatic responses to O2, CO2, and temperature; in particular, the interaction of globin-5 with soluble guanylate cyclase-35 is pivotal for the adaptation to either low or high O2 levels (Abergel et al, 2016;Kodama-Namba et al, 2013).…”

Comparison of the chemical composition and antioxidant stress ability of polysaccharides from Auricularia auricula under different drying methods

“…According to the methods of Xu 16 and Ren et al , 19 the lifespan assay was carried out after the L4 synchronized worms were cultured at 20 °C for 24 h. The samples (0.4 mg mL −1 and 0.8 mg mL −1 ) with 5-fluorouracil were added to the NGMs, and the worms were further cultured for 48 h. Then, the worms were transferred to 96-well plates with methyl viologen (200 μL, 70 mmol L −1 ) or H 2 O 2 (200 μL, 4 mmol L −1 ) and cultured at 20 °C. The number of dead worms was recorded every 60 min.…”