GLC Determination of Sorbitol in Irrigating Solutions

N-Methylimidazole-catalyzed acetylation of hydroxy compounds prior to gas chromatographic separation and determination

“…In Equation 1, the first term accounts for longitudinal diffusion and the second for nonequilibrium. If we make the reasonable assumption that longitudinal diffusion is negligible, and use Equation 2for in the second term, we have H = (R3/9)w2{u)/D (3) or, with R = Vo/V, = { /VT)3u:2(v)/9D (4) This equation illustrates a striking dependence of H on elution volume Vr, showing that H decreases rapidly as Vr increases. A closer scrutiny of the situation shows that D generally has a weak inverse dependence on Vr, which somewhat subtracts from the cube dependence of the first term.…”

“…For these purposes, we use the subtechnique of sedimentation FFF for our experimental work, although we expect similar results for the other subtechniques of FFF. We note that the flow programming proposed here is intended to complement field-strength programming, which we have discussed in several papers (3)(4)(5). It is also related closely to solvent programming (3), because both entail changes in the state of the incoming solvent.…”

“…We note that the flow programming proposed here is intended to complement field-strength programming, which we have discussed in several papers (3)(4)(5). It is also related closely to solvent programming (3), because both entail changes in the state of the incoming solvent. Of the three approaches, field-strength programming is perhaps the most versatile and direct.…”

“…Reaction times tend to be inconveniently long; the following are typical conditions. Sorbitol and mannitol are acetylated by refluxing for 1 h in 1:1 Ac20/pvr (2,3) or by heating in 5:1 Ac20/pvr at 100 °C for 30 min (4).…”