Gli2 is required for the induction and migration of Xenopus laevis neural crest

Cerrizuela, Santiago; Vega-Lopez, Guillermo A.; Palacio, María Belén; Tríbulo, Celeste; Aybar, Manuel J.

doi:10.1016/j.mod.2018.07.010

Cited by 13 publications

(10 citation statements)

References 105 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another transcription factor, Gli2, has been recently implicated in neural crest induction in Xenopus . Through gain‐of‐function and loss‐of‐function analyses, Gli2 was found to affect the expression of neural plate border and neural crest genes including pax3 , zic1 , msx1 , snai2 , foxd3 , and sox10 (Cerrizuela, Vega‐Lopez, Palacio, Tribulo, & Aybar, ). It remains to be seen how Gli2 mediated responses integrates with other signaling cascades (such as Wnt/β‐catenin and FGF signaling) that function in the regulation of these genes.…”

Section: Transcriptional Network Underlying Neural Crest Cell Formationmentioning

confidence: 99%

Specification and formation of the neural crest: Perspectives on lineage segregation

Prasad

Charney

Garcı́a-Castro

2019

Genesis

View full text Add to dashboard Cite

Summary The neural crest is a fascinating embryonic population unique to vertebrates that is endowed with remarkable differentiation capacity. Thought to originate from ectodermal tissue, neural crest cells generate neurons and glia of the peripheral nervous system, and melanocytes throughout the body. However, the neural crest also generates many ectomesenchymal derivatives in the cranial region, including cell types considered to be of mesodermal origin such as cartilage, bone, and adipose tissue. These ectomesenchymal derivatives play a critical role in the formation of the vertebrate head, and are thought to be a key attribute at the center of vertebrate evolution and diversity. Further, aberrant neural crest cell development and differentiation is the root cause of many human pathologies, including cancers, rare syndromes, and birth malformations. In this review, we discuss the current findings of neural crest cell ontogeny, and consider tissue, cell, and molecular contributions toward neural crest formation. We further provide current perspectives into the molecular network involved during the segregation of the neural crest lineage.

show abstract

Section: Transcriptional Network Underlying Neural Crest Cell Formationmentioning

confidence: 99%

Specification and formation of the neural crest: Perspectives on lineage segregation

Prasad

Charney

Garcı́a-Castro

2019

Genesis

View full text Add to dashboard Cite

show abstract

“…The role of Hedgehog signaling in NB remains poorly understood and, as in the case of Wnt signaling, there is conflicting evidence; two studies have suggested its oncogenic potential (Mao et al, 2009; Xu et al, 2012), whereas others have reported an influence on differentiation of NB cells (Williams et al, 2000; Souzaki et al, 2010). A role for Hedgehog signaling in cephalic and trunk NCC differentiation has been reported (Calloni et al, 2007), and in Xenopus, the Hedgehog transcription factor Gli2 was shown to be necessary for NCC specification and migration (Cerrizuela et al, 2018). In the context of NB, our previous studies suggested that WMG-2 genes were correlated with Hedgehog signaling and are suppressed by MYCN (Szemes et al, 2018).…”

Section: Is Wnt Signaling a Major Determinant Of Nc And Nb Cell Fate?mentioning

confidence: 99%

Wnt Signaling Is a Major Determinant of Neuroblastoma Cell Lineages

Szemes

Greenhough

Malik

2019

Front. Mol. Neurosci.

View full text Add to dashboard Cite

The neural crest (NC), which has been referred to as the fourth germ layer, comprises a multipotent cell population which will specify diverse cells and tissues, including craniofacial cartilage and bones, melanocytes, the adrenal medulla and the peripheral nervous system. These cell fates are known to be determined by gene regulatory networks (GRNs) acting at various stages of NC development, such as induction, specification, and migration. Although transcription factor hierarchies and some of their interplay with morphogenetic signaling pathways have been characterized, the full complexity of activities required for regulated development remains uncharted. Deregulation of these pathways may contribute to tumorigenesis, as in the case of neuroblastoma, a frequently lethal embryonic cancer thought to arise from the sympathoadrenal lineage of the NC. In this “Hypothesis and Theory” article, we utilize the next generation sequencing data from neuroblastoma cells and tumors to evaluate the possible influences of Wnt signaling on NC GRNs and on neuroblastoma cell lineages. We propose that Wnt signaling is a major determinant of regulatory networks that underlie mesenchymal/neural crest cell (NCC)-like cell identities through PRRX1 and YAP/TAZ transcription factors. Furthermore, Wnt may also co-operate with Hedgehog signaling in driving proneural differentiation programmes along the adrenergic (ADRN) lineage. Elucidation of Signaling Regulatory Networks can augment and complement GRNs in characterizing cell identities, which may in turn contribute to the design of improved therapeutics tailored to primary and relapsing neuroblastoma.

show abstract

“…Anastasiadi and Piferrer [83] showed that the domestication process in D. labrax resulted notably in genome-wide methylation differences for genes involved in the nervous system and neural crest cell differentiation. In our study, GLI2 [174], PBX1 [175], DLG1 [176] and FOXJ3 [177] are DMC-related genes involved in neural crest cell proliferation and differentiation. ROBO3 is involved in the immune response, but also implied in early neurogenesis [178], as well as NPAS3 [179].…”

Section: Family Effect Development and Few Individualsmentioning

confidence: 95%

Genome-Wide Epigenomic Stress Response in the European Sea Bass (Dicentrarchus Labrax, L.)

Krick

DESMARAIS

Σαμαράς

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: While the stress response inspired genome-wide epigenetic studies in vertebrate models, it remains mostly ignored in fish. We modified the epiGBS (epiGenotyping By sequencing) technique to explore changes in genome-wide cytosine methylation to a repeated acute stress challenge in the nucleated red blood cells (RBCs) of the European sea bass (Dicentrarchus labrax). This species is widely studied in both the natural and farmed environments, including issues regarding health and welfare.Results: We retrieved 501,108,033 sequencing reads after trimming, with a mean mapping efficiency of 73.0% (unique best hits). Fifty-seven differentially methylated cytosines (DMCs) close to 51 distinct stress-related genes distributed on 17 of 24 linkage groups (LGs) were detected between RBCs of pre- and post-stress individuals. Literature surveys indicated that thirty-eight of these genes were previously reported as differentially expressed in the brain of zebrafish, most of them involved in stress coping differences. DMC-related genes associated to the Brain Derived Neurotrophic Factor, a protein that favors stress adaptation and fear memory, are especially relevant.Conclusion: We provide an improved epiGBS protocol with increased multiplexing and sequencing capacities that offer new opportunities to improve data acquisition and to investigate important biological processes at a genome-wide level, such as the stress response. Minimally invasive RBCs deserve more attention to investigate the epigenetic response to stress without sacrificing fish.

show abstract

Gli2 is required for the induction and migration of Xenopus laevis neural crest

Cited by 13 publications

References 105 publications

Specification and formation of the neural crest: Perspectives on lineage segregation

Specification and formation of the neural crest: Perspectives on lineage segregation

Wnt Signaling Is a Major Determinant of Neuroblastoma Cell Lineages

Genome-Wide Epigenomic Stress Response in the European Sea Bass (Dicentrarchus Labrax, L.)

Contact Info

Product

Resources

About