Gliadin and Reticulin Antibodies in Childhood Coeliac Disease

Unsworth, D J; Walker-Smith, J A; Holborow, E. J.

doi:10.1016/s0140-6736(83)91411-3

Cited by 91 publications

(75 citation statements)

References 9 publications

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“…In recent years, data have been produced on their utility in assess ing CCD patients. By using crude gliadin as antigen, it has been reported that IFL-and ELISA-AGA are closely related to CCD in active phase [4,5]; however, a high preva lence of these antibodies has also been found in other gastroenterological disorders of childhood [1,17], In a previous study [3] we were able to detect IFL-and/or ELISA-AGA in all the 30 children with untreated or glu ten-challenged CCD studied. The prevalence of 'false-positives' was very low (8%) con fined to occasional cases of intestinal disor ders with immunological abnormalities (Crohn's disease, ulcerative colitis, IgA defi ciency).…”

Section: Discussionmentioning

confidence: 73%

Antibodies to Gliadin in Adult Coeliac Disease and Dermatitis herpetiformis

et al. 1984

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Antibodies to gliadin, searched for by indirect immunofluorescence and a micro-ELISA, were detected in 16 (64%) of 25 sera from patients with adult coeliac disease and in 13 (45%) of 29 with dermatitis herpetiformis. Although the sensitivity of the two tests was relatively low in the whole groups, it increased when only cases with severe jejunum abnormalities were considered (93% for coeliac disease and 81% for dermatitis herpetiformis). A significant correlation was found between antigliadin antibodies and the severity of jejunum damage in both diseases. Moreover, most coeliac and dermatitis herpertiformis patients with antigliadin antibodies were on normal diet. The specificity of the tests was 100% for the immunofluorescence and fairly good for the micro-ELISA, as only 5(11 %) of the 46 disease control patients (Crohn’s disease, ulcerative colitis) were positive for antigliadin antibodies. R1 -reticulin antibody test was equally specific but less sensitive in both groups. We conclude that antigliadin antibodies are useful in the diagnosis of patients with active adult coeliac disease and dermatitis herpetiformis with gluten-sensitive enteropathy. Moreover, the two tests make it possible to monitor the compliance to gluten-free diet in both diseases.

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Section: Discussionmentioning

confidence: 73%

Antibodies to Gliadin in Adult Coeliac Disease and Dermatitis herpetiformis

et al. 1984

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“…[1][2][3] Its clinical presentation is highly variable, ranging from silent to fully expressed intestinal malabsorption. 4 A strong association with HLA-DQ2 5 and a high prevalence of autoimmune diseases, such as insulin dependent diabetes mellitus and Hashimoto's thyroiditis, are typical CD features.…”

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confidence: 99%

Immune Reaction Against Cytoskeleton in Coeliac Disease

Clemente

Frau

Brusco

et al. 1999

Journal of Pediatric Gastroenterology & Nutrition

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Background-The cytoskeleton actin network of intestinal microvilli has been found to be rapidly impaired after gluten challenge in coeliac disease (CD). The aim of this study was to investigate the presence of an immune reaction towards cytoskeleton structures such as actin filaments in CD. Methods-Eighty three antiendomysial antibody positive CD patients (52 children and 31 adults) were studied at our outpatient clinics from 1996 to 1998 using indirect immunofluorescence, ELISA, and western blotting for antiactin (AAA) and antitissue transglutaminase (TGA) antibodies before and after a gluten free diet (GFD). Sixteen patients with smooth muscle antibody positive autoimmune hepatitis, 21 with inflammatory bowel diseases, seven with small bowel bacterial overgrowth, and 60 healthy subjects were studied as controls. Results-Fifty nine of 83 CD patients (28/31 adults (90.3%); 31/52 children (59.6%)) were positive for IgA and/or IgG AAA. Seventy seven (92.7%) were positive for IgA TGA. IgA AAA were strongly correlated with more severe degrees of intestinal villous atrophy (p<0.0001; relative risk 86.17). After a GFD, AAA became undetectable within five months. Conclusions-Apart from the immune reaction against the extracellular matrix, we have described an immune reaction against the cytoskeleton in both children and adults with CD. As AAA are strongly associated with more severe degrees of villous atrophy, they may represent a useful serological marker of severe intestinal atrophy in CD. (Gut 2000;47:520-526)

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“…Recently a series of simple and non invasive tests have been proposed as an alternative to jejunal biopsy, for ameliorating the diagnosis of CD during gluten challenge (UNSWORTH et al, 1983;GRECO et al, 1987). Nevertheless, at present, no test has shown sufficient effectiveness in substituting small intestinal biopsy in making the clinical diagnosis (WALKER-SMITH et al, 1990).…”

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confidence: 99%