Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking

Syed, Durafshan Sakeena; Gowda, Swetha B. M.; Reddy, O Venkateswara; Reichert, Heinrich; VijayRaghavan, K.

doi:10.7554/elife.11572

Cited by 28 publications

(28 citation statements)

References 49 publications

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“…Slit-Robo, Netrin-Fra, and Sema-Plexin signaling have been shown to control motor neuron dendrite targeting in Drosophila , and rescue experiments suggest that these guidance receptors act cell autonomously in this process (Brierley et al, 2009; Mauss et al, 2009; Syed et al, 2016). In addition, the initial targeting of motor neuron dendrites in the embryo is largely unaffected by manipulations that affect the position or the activity of pre-synaptic axons or the presence of muscles, suggesting that this process is likely under the control of cell autonomous factors, though these remained unidentified (Landgraf et al, 2003; Mauss et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Islet Coordinately Regulates Motor Axon Guidance and Dendrite Targeting through the Frazzled/DCC Receptor

Santiago¹,

Bashaw²

2017

Cell Reports

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Summary Motor neuron axon targeting in the periphery is correlated with the positions of motor neuron inputs in the CNS, but how these processes are coordinated to form a myotopic map remains poorly understood. We show that the LIM homeodomain factor Islet (Isl) controls targeting of both axons and dendrites in Drosophila motor neurons through regulation of the Frazzled (Fra)/DCC receptor. Isl is required for fra expression in ventrally-projecting motor neurons, and isl and fra mutants have similar axon guidance defects. Single-cell labeling indicates that isl and fra are also required for dendrite targeting in a subset-specific way. Finally, over-expression of Fra rescues axon and dendrite targeting defects in isl mutants. These results indicate that Fra acts downstream of Islet in both the periphery and the CNS, demonstrating how a single regulatory relationship is used in multiple cellular compartments to coordinate neural circuit wiring.

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Section: Discussionmentioning

confidence: 99%

Islet Coordinately Regulates Motor Axon Guidance and Dendrite Targeting through the Frazzled/DCC Receptor

Santiago¹,

Bashaw²

2017

Cell Reports

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“…For the VGN6341-LexA , the 505 bp fragment of the dvGlut gene enhancer as in VGN6341-GAL4 (Syed et al, 2016) was PCR amplified from wild-type DNA, the sequence was verified and cloned into pDONR-221-p1-p5r (Invitrogen) to get an entry clone by performing the BP reaction. This entry clone along with the LexA entry clone and the destination vector were combined in an LR reaction to generate VGN6341-LexA .…”

Section: Methodsmentioning

confidence: 99%

Drosophila larval to pupal switch under nutrient stress requires IP3R/Ca2+ signalling in glutamatergic interneurons

Jayakumar

Richhariya

Reddy

et al. 2016

eLife

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Neuronal circuits are known to integrate nutritional information, but the identity of the circuit components is not completely understood. Amino acids are a class of nutrients that are vital for the growth and function of an organism. Here, we report a neuronal circuit that allows Drosophila larvae to overcome amino acid deprivation and pupariate. We find that nutrient stress is sensed by the class IV multidendritic cholinergic neurons. Through live calcium imaging experiments, we show that these cholinergic stimuli are conveyed to glutamatergic neurons in the ventral ganglion through mAChR. We further show that IP3R-dependent calcium transients in the glutamatergic neurons convey this signal to downstream medial neurosecretory cells (mNSCs). The circuit ultimately converges at the ring gland and regulates expression of ecdysteroid biosynthetic genes. Activity in this circuit is thus likely to be an adaptation that provides a layer of regulation to help surpass nutritional stress during development.DOI: http://dx.doi.org/10.7554/eLife.17495.001

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“…Importantly, Semaphorins and Plexins continue to be expressed in the mature brain where their function remains largely unknown 12–16 . Semaphorins, have been documented as synaptic signaling proteins, but this activity has been limited to the control of neuroanatomical synapse formation and elimination 15–17 . Here, we demonstrate that Sema-Plexin signaling achieves a retrograde, trans-synaptic control of presynaptic neurotransmitter release and homeostatic plasticity.…”

mentioning

confidence: 99%

Retrograde semaphorin–plexin signalling drives homeostatic synaptic plasticity

Orr

Fetter

Davis

2017

Nature

100

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Homeostatic signaling systems ensure stable, yet flexible neural activity and animal behavior1–4. Defining the underlying molecular mechanisms of neuronal homeostatic signaling will be essential in order to establish clear connections to the causes and progression of neurological disease. Presynaptic homeostatic plasticity (PHP) is a conserved form of neuronal homeostatic signaling, observed in organisms ranging from Drosophila to human1,5. Here, we demonstrate that Semaphorin2b (Sema2b) is target-derived signal that acts upon presynaptic PlexinB (PlexB) receptors to mediate the retrograde, homeostatic control of presynaptic neurotransmitter release at the Drosophila neuromuscular junction. Sema2b-PlexB signaling regulates the expression of PHP via the cytoplasmic protein Mical and the oxoreductase-dependent control of presynaptic actin6,7. During neural development, Semaphorin-Plexin signaling instructs axon guidance and neuronal morphogenesis8–10. Yet, Semaphorins and Plexins are also expressed in the adult brain11–16. Here we demonstrate that Semaphorin-Plexin signaling controls presynaptic neurotransmitter release. We propose that Sema2b-PlexB signaling is an essential platform for the stabilization of synaptic transmission throughout life.

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Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking

Cited by 28 publications

References 49 publications

Islet Coordinately Regulates Motor Axon Guidance and Dendrite Targeting through the Frazzled/DCC Receptor

Islet Coordinately Regulates Motor Axon Guidance and Dendrite Targeting through the Frazzled/DCC Receptor

Drosophila larval to pupal switch under nutrient stress requires IP3R/Ca2+ signalling in glutamatergic interneurons

Retrograde semaphorin–plexin signalling drives homeostatic synaptic plasticity

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