Glial Cells of the Central Nervous System in the Crab Ucides cordatus

Allodi, Silvana; Silva, Simone Florim da; Taffarel, M

doi:10.2307/3227058

Cited by 19 publications

(10 citation statements)

References 18 publications

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“…By qualitative and quantitative ultrastructural analyses, we described the features and time course of the protocerebral tract (PCT) events following extirpation of the optic stalk. In addition to the typical features of degeneration observed within the nerve fibers, we saw cells with the morphology of glial cells previously described in the same species (Allodi et al 1999), and granule cells dispersed among the degenerating axons. This led us to hypothesize that granule cells resembling blood cells (hemocytes) share with glial cells the function of phagocytosis of cellular debris from the tract degeneration.…”

Section: Introductionsupporting

confidence: 62%

Identity of the cells recruited to a lesion in the central nervous system of a decapod crustacean

et al. 2010

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In a previous study, we analyzed and described the features of the degeneration of the protocerebral tract (PCT) of the crustacean Ucides cordatus, after the extirpation of the eyestalk. In that study, among axons with axoplasmic degeneration, cells with granules resembling blood cells (hemocytes) were seen. Therefore, in the present study, we characterized the circulating hemocytes and compared them with the cells recruited to a lesion, which was produced as in the former study. Using histochemistry, immunohistochemistry, and electron microscopy (transmission and scanning), we confirmed that circulating and recruited cells display a similar morphology. Therefore, in the crab, hemocytes were attracted to the lesion site in the acute stage of degeneration, appearing near local glial cells that showed signs of being responsive. Some of the attracted hemocytes displayed a morphology that was considered to be possibly activated blood cells. Also, the cells that migrated to the injured PCT displayed features, such as the presence of hydrolytic enzymes and an ability to phagocytize neural debris, similar to those of vertebrates. In summary, our results indicate that hemocytes were not only phagocytizing neural debris together with glial cells but also that they may be concerned with creating a favorable environment for regenerating events.

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Section: Introductionsupporting

confidence: 62%

Identity of the cells recruited to a lesion in the central nervous system of a decapod crustacean

et al. 2010

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“…A difference between the axoplasm of vertebrates and invertebrates is that, while the axoplasm of vertebrates contains conspicuous NFs, these cytoskeleton elements were not observed as visible typical intermediate filaments in arthropods by electron microscopy (Allodi et al, 1999;da Silva et al, 2001;Goldstein and Gunawardena, 2000;Heuser and Doggenweiler, 1966). However, as reported previously (Corrêa et al, 2004), we found NF proteins in regions corresponding to the MT side-arms in the crustacean protocerebral tract (PCT).…”

Section: Introductionsupporting

confidence: 57%

“…This tract has been used in our laboratory to study glial-axon relationships and specific issues related to the cell biology of the crustacean axoplasm (Allodi et al, 1999;Corrêa et al, 2004Corrêa et al, , 2005. In the present report, the PCT was chosen because it is mostly composed of axons, and is therefore a suitable model for the approach used in this study.…”

Section: Introductionmentioning

confidence: 99%

Electron microscopy and morphometric analyses of microtubules in two differently sized types of axons in the protocerebral tract of a crustacean

Corrêa

Silva

Pereira

et al. 2007

Microscopy Res & Technique

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Despite several reports on the morphology and functions associated with the morphometry of the vertebrate axoplasm cytoskeleton, the subject has not been thoroughly explored in invertebrates. In vertebrates, among many other functions, microtubules (MTs) serve as scaffolding for axon assembly, and neurofilaments (NFs) as the elements that determine the axon caliber. Intermediate filaments have never been described by electron microscopy in arthropods, although NF proteins have been revealed in the MT side-arms of the axoplasm of certain species, such as the crab Ucides cordatus. Thus, it is not known which elements of the cytoskeleton of invertebrates are responsible for determination of the axon caliber. We studied, by electron microscopy and morphometric analyses, the MT and axon area variability in differently sized axons of the protocerebral tract of the crab Ucides cordatus. Our results revealed differences in the distance between MTs, in MT density and number, and in the areas of differently sized axons. The number of MTs increases with the axon area, but this relationship is not directly proportional. Therefore, MT density is greater in smaller axons than in medium axons, similar to the morphometry of the vertebrate axon MT. The distance between MTs is, however, directly related to the axonal area. On the basis of the results shown here, and on previous reports by us and others, we suggest that MTs may be involved in the determination of the axon caliber, possibly due to the presence of NF proteins found in the side-arms. Microsc. Res. Tech. 71:214-219, 2008. V

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“…They supply neurons with energy by donating glycogen and other energy substrates for Na + /K + -ATPases, maintain the concentration of Ca 2+ ions and pH in the extracellular medium, regulate synapse formation and nerve pulse conduction, and protect neurons from oxidative stress [5]. GP metabolism is closely related to metabolism of The appearance of glial wrappings observed by us is comparable with the results of studies on crab axon [8,9] lacking myelin sheaths. However, periaxonal sheaths in crab axons appear due to wrapping with GP containing glycogen.…”

Section: Resultssupporting

confidence: 85%

Role of Glycogen in Processes of Cerebellar Glial Cells under Conditions of Its Damage with Sodium Nitrite

2010

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Ultrastructure of processes of glial cell, astrocytes of the molecular layer of cerebellar cortex in Rana temporaria frog, under conditions of damage to the cerebellum caused by NO-generating compound sodium nitrite was studied under an electron microscope. It was found that astrocytes have at least two types of processes: the first (fibrillar) primarily contained numerous fibrils and few glycogen granules and the second (granular) primarily containing glycogen granules. In the presence of NO-generating compound in toxic doses, fibrillar processes are damaged or completely degrade more rapidly than granular ones. The processes containing glycogen can protect both damaged synapses and individual synaptic buttons by forming a compact structure, wrapping, around them. We analyzed the possible role of glycogen of cerebellar glial cell processes in neuroglial interactions in the presence of sodium nitrite.

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Glial Cells of the Central Nervous System in the Crab Ucides cordatus

Cited by 19 publications

References 18 publications

Identity of the cells recruited to a lesion in the central nervous system of a decapod crustacean

Identity of the cells recruited to a lesion in the central nervous system of a decapod crustacean

Electron microscopy and morphometric analyses of microtubules in two differently sized types of axons in the protocerebral tract of a crustacean

Role of Glycogen in Processes of Cerebellar Glial Cells under Conditions of Its Damage with Sodium Nitrite

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