Glioma therapy using tumor homing and penetrating peptide-functionalized PEG–PLA nanoparticles loaded with paclitaxel

Hu, Quanyin; Gao, Xiaoling; Gu, Guangzhi; Kang, Ting; Tu, Yifan; Liu, Zhongyang; Song, Qing; Yao, Lei; Pang, Zhiqing; Jiang, Xinguo; Chen, Hongzhuan; Chen, Jun

doi:10.1016/j.biomaterials.2013.04.025

Cited by 155 publications

(114 citation statements)

References 47 publications

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“…44 Recently, tLyp-1-conjugated iron oxide nanoparticles, 44 PEG-PLA (polyethylene glycol-polylactic acid) nanoparticles, 50 and cationic liposomes 51 were constructed by different groups and showed robust and selective homing to tumors, penetrating from the blood vessels into the tumor parenchyma. To examine the characteristics of TMSG as a targeting nanoparticle to human breast cancer MDA-MB-231 cells, optical imaging experiments about endocytosis of fluorescent-labeled nanoparticles were performed.…”

Section: Targeting Uptaking Of Tmsg By Tumor Cellsmentioning

confidence: 99%

Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser

Liu¹,

Xu²,

Chen³

et al. 2015

IJN

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Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

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Section: Targeting Uptaking Of Tmsg By Tumor Cellsmentioning

confidence: 99%

Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser

Liu¹,

Xu²,

Chen³

et al. 2015

IJN

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“…Aliquots of medium (1.0 mL) was collected at determined times (1,2,4,6,8,12, and 24 h) and replaced with an equivalent volume of fresh medium (37°C). The concentration of DOX released from the mixed micelles was measured with a UV/visible spectrophotometer as described previously.…”

Section: In Vitro Ph-sensitive Releasementioning

confidence: 99%

“…1 Development of nanocarrier system has shown promise in enhancing tumor selectivity and decreasing adverse effects of anticancer drugs. 2,3 The nanoparticles can perform with hydrophilic surfaces in order to avoid opsonization and removal by the mononuclear phagocytic cells, and they are small enough to take the advantage of the leaky new vasculature and poor lymphatic systems of tumors for accumulation into tumor sites. 4 This phenomenon is known as the enhanced permeability and retention (EPR) effect.…”

Section: Introductionmentioning

confidence: 99%

Decoration of pH-sensitive copolymer micelles with tumor-specific peptide for enhanced cellular uptake of doxorubicin

Chen¹,

Long²,

Qiu³

et al. 2016

IJN

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To improve the targeting efficacy of hyaluronic acid (HA)-based micelles, pH-sensitive mixed micelles based on HA-g-poly(L-histidine) (PHis) and d-α-tocopheryl polyethylene glycol 2000 copolymers were prepared and decorated with human epidermal growth factor receptor 2 (Her2) peptide, a tumor cell-specific peptide ligand, on their surface. The doxorubicin-loaded micelles (HA-PHis/peptide–d-α-tocopheryl polyethylene glycol 2000 mixed micelles [PHTM]) were characterized to have a unimodal size distribution and pH-dependent drug release pattern. In vitro tumor targeting studies demonstrated that PHTM exhibited the pronounced cytotoxicity and efficient internalization in MDA-MB-231 cells overexpressing CD44 and Her2 receptors. In vivo investigation into micelles in MDA-MB-231 tumor-bearing mice confirmed that PTHM could reach the tumor site more effectively and exert excellent tumor killing activity. In general, Her2 peptide decoration can enhance the selective cytotoxicity and antitumor activity of HA-based micelles.

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“…expressed, were used as the models of tumor cells and tumor neovascular endothelial cells, respectively. 20,22,23,47,48 Low cytotoxicity of the carrier itself is a primary concern in the development of a drug delivery system. Figure 6A and B shows the viability of MDA-MB-231 cells and HUVECs incubated with HMSN, pHMSN, or tHMSN for 48 hours (as determined by CCK-8 assay).…”

Section: Evaluation Of Drug-loading and Drug-release Propertiesmentioning

confidence: 99%

“…tLyp-1 (sequence CGNKRTR), 20,21 a newly reported heptapeptide which are screened by phage display library, is a ligand which is selectively targeted both to NRP1 and NRP2. Therefore, tLyp-1-decorated nanoparticles may be expected to have dual-targeting functions, and previous studies 22,23 have shown that such polymer nanoparticles have precise dual-targeting efficacy and penetrate extensively into tumor parenchyma.…”

mentioning

confidence: 99%

Single peptide ligand-functionalized uniform hollow mesoporous silica nanoparticles achieving dual-targeting drug delivery to tumor cells and angiogenic blood vessel cells

Liu¹,

Chen²,

Xu³

et al. 2015

IJN

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Background The purpose of this study was to construct hollow mesoporous silica nanoparticles (HMSN) decorated with tLyp-1 peptide (tHMSN) for dual-targeting drug delivery to tumor cells and angiogenic blood vessel cells. Methods HMSN were synthesized de novo using a novel cationic surfactant-assisted selective etching strategy and were then modified with tLyp-1. Multiple methods, including transmission electron microscopy, X-ray photoelectron spectroscopy, thermogravimetric analysis, bicinchoninic acid assay, and nitrogen adsorption and desorption isotherms, were used to characterize the tHMSN. Doxorubicin were chosen as the model cargo, and the uptake of doxorubicin-loaded tHMSN into MDA-MB-231 cells and human umbilical vein endothelial cells (HUVECs), as models of tumor cells and tumor neovascular endothelial cells, respectively, were observed and detected by confocal laser scanning microscopy and flow cytometry. An in vitro pharmacodynamic study and a study of the mechanism via which the nanoparticles were endocytosed were also performed. Results HMSN with a highly uniform size and well oriented mesopores were synthesized. After tHMSN were characterized, enhanced uptake of the cargo carried by tHMSN into MDA-MB-231 cells and HUVECs compared with that of their unmodified counterparts was validated by confocal laser scanning microscopy and flow cytometry at the qualitative and quantitative levels, respectively. Further, the pharmacodynamic study suggested that, compared with their unmodified counterparts, doxorubicin-loaded tHMSN had an enhanced inhibitory effect on MDA-MB-231 cells and HUVECs in vitro. Finally, a preliminary study on the mechanism by which the nanoparticles were endocytosed indicated that the clathrin-mediated endocytosis pathway has a primary role in the transport of tHMSN into the cytoplasm. Conclusion tHMSN might serve as an effective active targeting nanocarrier strategy for anti-mammary cancer drug delivery.

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Glioma therapy using tumor homing and penetrating peptide-functionalized PEG–PLA nanoparticles loaded with paclitaxel

Cited by 155 publications

References 47 publications

Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser

Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser

Decoration of pH-sensitive copolymer micelles with tumor-specific peptide for enhanced cellular uptake of doxorubicin

Single peptide ligand-functionalized uniform hollow mesoporous silica nanoparticles achieving dual-targeting drug delivery to tumor cells and angiogenic blood vessel cells

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Glioma therapy using tumor homing and penetrating peptide-functionalized PEG–PLA nanoparticles loaded with paclitaxel

Cited by 155 publications

References 47 publications

Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser

Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser

Decoration of pH-sensitive copolymer micelles with tumor-specific peptide for enhanced cellular uptake of doxorubicin

Single peptide ligand-functionalized uniform hollow mesoporous silica nanoparticles achieving dual-targeting drug delivery to tumor cells and&nbsp;angiogenic blood vessel cells

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Single peptide ligand-functionalized uniform hollow mesoporous silica nanoparticles achieving dual-targeting drug delivery to tumor cells and angiogenic blood vessel cells