Global Ablation of the Mouse Rab11a Gene Impairs Early Embryogenesis and Matrix Metalloproteinase Secretion

Yu, Shiyan; Yehia, Ghassan; Wang, Juanfei; Stypulkowski, Ewa; Sakamori, Ryotaro; Ping, Jiang; Hernandez-Enriquez, Berenice; Tran, Tracy S.; Bonder, Edward M.; Guo, Wei; Gao, Nan

doi:10.1074/jbc.m113.538223

Cited by 41 publications

(54 citation statements)

References 77 publications

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“…Blastocyst hatching in vitro is brought about by increasing pressure from the expanding blastocyst combined with zona-thinning embryo-secreted proteases, where implantation serine protease 1 (ISP1, also known as strypsin) is thought to play a vital role (Ichikawa et al, 1985;O'Sullivan et al, 2001;Perona and Wassarman, 1986;Sawada et al, 1990;Sharma et al, 2006). An increase in hatching rate may be due to an overall improvement in embryo health, but during culture proteases are secreted into the culture media (Hwang et al, 2000;Martinez-Hernandez et al, 2011;Sawada et al, 1990;Whiteside et al, 2001;Yu et al, 2014), and could assist in the zona degradation and hatching of neighbouring embryos. Also, it has been suggested that protease production for hatching may be stimulated by growth factors and cytokines (Seshagiri et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Kelley

Gardner

2016

Reproductive BioMedicine Online

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Embryos are routinely cultured individually, although this can reduce blastocyst development. Culture in atmospheric (20%) oxygen is also common, despite multiple detrimental effects on embryos. Although frequently occurring together, the consequences of this combination are unknown. Mouse embryos were cultured individually or grouped, under physiological (5%) or atmospheric (20%) oxygen. Embryos were assessed by time-lapse and blastocyst cell allocation. Compared with the control group (5% oxygen group culture), 5-cell cleavage (t5) was delayed in 5% oxygen individual culture and 20% oxygen group culture (59.91 ± 0.23, 60.70 ± 0.29, 63.06 ± 0.32 h post-HCG respectively, P < 0.05). Embryos in 20% oxygen individual culture were delayed earlier (3-cell cleavage), and at t5 cleaved later than embryos in other treatments (66.01 ± 0.40 h, P < 0.001), this delay persisting to blastocyst hatching. Compared with controls, hatching rate and cells per blastocyst were reduced in 5% oxygen single culture and 20% oxygen group culture (134.1 ± 3.4, 104.5 ± 3.2, 73.4 ± 2.2 cells, P < 0.001), and were further reduced in 20% oxygen individual culture (57.0 ± 2.8 cells, P < 0.001), as was percentage inner cell mass. These data indicate combining individual culture and 20% oxygen is detrimental to embryo development.

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Section: Discussionmentioning

confidence: 99%

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Kelley

Gardner

2016

Reproductive BioMedicine Online

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“…Interestingly, Rab8a KO mice also show deficits in microvillus formations in the intestines and these defects are augmented in Rab8a/Rab8b double KO mice [38,39 • ]. While Rab11a germline KO mice are embryonic lethal [40], targeted KO of Rab11a in intestinal enterocytes leads to short apical microvilli and deficits in TLR9 recycling [41–43]. …”

Section: Components Of the Apical Recycling System Influence The Mainmentioning

confidence: 99%

Recycling endosomes

Goldenring

2015

Current Opinion in Cell Biology

135

118

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The endosomal membrane recycling system represents a dynamic conduit for sorting and re-exporting internalized membrane constituents. The recycling system is composed of multiple tubulovesicular recycling pathways that likely confer distinct trafficking pathways for individual cargoes. In addition, elements of the recycling system are responsible for assembly and maintenance of apical membrane specializations including primary cilia and apical microvilli. The existence of multiple intersecting and diverging recycling tracks likely accounts for specificity in plasma membrane recycling trafficking.

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“…The conditional Rab11a floxed allele mice were derived from homologous recombination in embryonic stem cells as described previously by Yu, et al (Yu et al, 2014a;Yu et al, 2014b). The Rab8a-knockout mice used for the study have also been described previously by Sakamori et al (Sakamori et al, 2012;Sato et al, 2007).…”

Section: Micementioning

confidence: 99%

Rab11a regulates Syntaxin 3 localization and microvillus assembly in enterocytes

Knowles

Weis

et al. 2015

Journal of Cell Science

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Rab11a is a key component of the apical recycling endosome that aids in the trafficking of proteins to the luminal surface in polarized epithelial cells. Utilizing conditional Rab11a-knockout specific to intestinal epithelial cells, and human colonic epithelial CaCo2-BBE cells with stable Rab11a knockdown, we examined the molecular and pathological impact of Rab11a deficiency on the establishment of apical cell polarity and microvillus morphogenesis. We demonstrate that loss of Rab11a induced alterations in enterocyte polarity, shortened microvillar length and affected the formation of microvilli along the lateral membranes. Rab11a deficiency in enterocytes altered the apical localization of syntaxin 3. These data affirm the role of Rab11a in apical membrane trafficking and the maintenance of apical microvilli in enterocytes.

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Global Ablation of the Mouse Rab11a Gene Impairs Early Embryogenesis and Matrix Metalloproteinase Secretion

Cited by 41 publications

References 77 publications

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Recycling endosomes

Rab11a regulates Syntaxin 3 localization and microvillus assembly in enterocytes

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