Global analysis of hematopoietic and vascular endothelial gene expression by tissue specific microarray profiling in zebrafish

Covassin, Laurence; Amigo, Julio D.; Suzuki, K.; Teplyuk, Viktor I.; Straubhaar, Juerg R.; Lawson, Nathan D.

doi:10.1016/j.ydbio.2006.08.020

Cited by 113 publications

(106 citation statements)

References 41 publications

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“…Embryo dissociation and FACS were performed as described previously by Covassin et al [44]. The GFP + and GFP − cells were separately collected from cmyb:EGFP and runx1:EGFP transgenic embryos (Zhang P and Liu F, unpublished data) using MoFlo XDP (Beckman).…”

Section: Zebrafish Embryo Dissociation and Facsmentioning

confidence: 99%

miR-142-3p regulates the formation and differentiation of hematopoietic stem cells in vertebrates

et al. 2013

Cell Res

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Previous studies on developmental hematopoiesis have mainly focused on signaling and transcription factors, while the appreciation of epigenetic regulation including that of microRNAs is recent. Here, we show that in zebrafish and mouse, miR-142-3p is specifically expressed in hematopoietic stem cells (HSCs). Knockdown of miR-142a-3p in zebrafish led to a reduced population of HSCs in the aorta-gonad-mesonephros (AGM) region as well as T-cell defects in the thymus. Mechanistically, miR-142a-3p regulates HSC formation and differentiation through the repression of interferon regulatory factor 7 (irf7)-mediated inflammation signaling. Finally, we show that miR-142-3p is also involved in the development of HSCs in mouse AGM, suggesting that it has a highly conserved role in vertebrates. Together, these findings unveil the pivotal roles that miR-142a-3p plays in the formation and differentiation of HSCs by repressing irf7 signaling.

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Section: Zebrafish Embryo Dissociation and Facsmentioning

confidence: 99%

miR-142-3p regulates the formation and differentiation of hematopoietic stem cells in vertebrates

et al. 2013

Cell Res

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“…Larval dissociation was performed as described (Covassin et al, 2006). Briefly, larvae were killed in a bath of ice-cold E3 and rinsed in calcium-free Ringer solution before trypsin (Invitrogen, final concentration of 0.25% trypsin-EDTA [ethylenediaminetetraacetic protein]) digestion.…”

Section: Facsmentioning

confidence: 99%

A chemical enterocolitis model in zebrafish larvae that is dependent on microbiota and responsive to pharmacological agents

Oehlers

Flores

Okuda

et al. 2010

Developmental Dynamics

120

168

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Inflammatory bowel disease (IBD) results from dysfunctional interactions between the intestinal immune system and microbiota, influenced by host genetic susceptibility. Because a key feature of the pathology is intestinal epithelial damage, potential disease factors have been traditionally analyzed within the background of chemical colitis models in mice. The zebrafish has greatly complemented the mouse for modeling aspects of disease processes, with an advantage for high content drug screens. Larval zebrafish exposed to the haptenizing agent trinitrobenzene sulfonic acid (TNBS) displayed impaired intestinal homeostasis and inflammation reminiscent of human IBD. There was a marked induction of pro-inflammatory cytokines, the degradative enzyme mmp9 and leukocytosis. Enterocolitis was dependent on microbiota and Toll-like receptor signaling, that can be ameliorated by antibiotic and anti-inflammatory drug treatments. This system will be useful to rapidly interrogate in vivo the biological significance of the IBD candidate genes so far identified and to carry out pharmacological modifier screens. Developmental Dynamics 240:288-298,

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“…They achieved this by expressing EGFP under the promoter control of a gene, which was uniquely expressed in the desired cell population. [44][45][46][47][48] Another significant drawback to defining cells of interest by their endogenous cell surface markers is that these markers may not be expressed at the earliest stages of differentiation. Onset of stem cell differentiation down a particular lineage is often defined by a transcription factor functioning as a master regulator.…”

Section: Discussionmentioning

confidence: 99%

Development of the “Three-step MACS”: a Novel Strategy for Isolating Rare Cell Populations in the Absence of Known Cell Surface Markers from Complex Animal Tissue

Lee¹,

Lufkin²

2012

J Biomol Tech

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To circumvent the difficulty of isolating specific cell populations by MACS from dissociated complex animal tissue, when their proportions reached levels similar to that of the background, we developed the "Three-step MACS" strategy. Cells of interest are defined by their expression of a particular gene(s) of interest rather by than their natural cell surface markers or size. A two-component transgenic cell surface protein, for two sequential rounds of MACS, is expressed under the promoter control of the endogenous gene of interest by means of gene targeting and the generation of transgenic tissue. An initial step to remove dead cells is also used. Here, we describe proof-of-concept experiments, using the biotin acceptor peptide (BAP)-low-affinity nerve growth factor receptor as the two-component protein. The first component, the BAP, can be biotinylated in specific subsets of cells expressing a particular gene by expressing the biotinylating enzyme, hBirA ϭ humanized BirA (hBirA), under the promoter control of another gene defining the specific subpopulation. We showed that a rare population of cells (1.1% of the 13.5 days postcoital mouse embryo) could be enriched to a sufficiently high purity (84.4%). From another sample with 0.1% of our cells of interest, we achieved a 40.3% pure sample. The low cost, speed, and technical ease of the Three-step MACS also make it scalable and hence, an ideal method for preparing sufficient quantities of biological samples for sensitive, high-throughput assays.

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Global analysis of hematopoietic and vascular endothelial gene expression by tissue specific microarray profiling in zebrafish

Cited by 113 publications

References 41 publications

miR-142-3p regulates the formation and differentiation of hematopoietic stem cells in vertebrates

miR-142-3p regulates the formation and differentiation of hematopoietic stem cells in vertebrates

A chemical enterocolitis model in zebrafish larvae that is dependent on microbiota and responsive to pharmacological agents

Development of the “Three-step MACS”: a Novel Strategy for Isolating Rare Cell Populations in the Absence of Known Cell Surface Markers from Complex Animal Tissue

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