Global analysis of tissue-differential gene expression patterns and functional regulation of rapid antler growth

Yao, Baojin; Zhang, Mei; Gao, Hongwei; Lu, Bocheng; Leng, Xiangyang; Zhao, Daqing; Zhao, Yu

doi:10.1007/s13364-018-0394-9

Cited by 11 publications

(7 citation statements)

References 62 publications

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“…Ba et al analyzed the gene expression patterns of these five layers and found the hub genes and signaling pathway related to chondrogenesis and osteogenesis 9 . Through RNA-seq methods, gene expression patterns of the skin, mesenchyme, pre-cartilage and cartilage also were analyzed and series of genes that contributed to chondrogenesis were identified 10 .…”

Section: Introductionmentioning

confidence: 99%

Comparative antler proteome of sika deer from different developmental stages

Zhang

Xing

2021

Sci Rep

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Antler is a special bone tissue that has the ability to regenerate completely periodically. It is the fastest growing bone in the animal kingdom. Antler provides a valuable research model for bone growth and mineralization. Antler grows longitudinally by endochondral ossification with their growth center located in its tip. Many scholars have carried out detailed studies on morphology and gene expression of antler tip. However, few scholars have analyzed the protein expression patterns of antler tip at different development stages. This study used label-free proteomics approach to analyze the protein expression dynamics of the antler tip in six developmental periods (15, 25, 45, 65, 100 and 130 days after the previous antler cast) and costal cartilage. In result, 2052 proteins were confidently quantified, including 1937 antler proteins and 1044 costal cartilage proteins. Moreover, 913 antler core proteins and 132 antler-special proteins were obtained. Besides, the stages special proteins and differentially expressed proteins (DEPs) in different development stages were analyzed. A total of 875 DEPs were determined by one-way AVOVA. It is found that the growth period (15, 25, 45 and 65 days) showed more up-regulated protein including several chondrogenesis-associated proteins (collagen types II, collagen types XI, HAPLN1, PAPSS1 and PAPSS2). In ossification stages, the up-regulated proteins related with lysosome (CTSD, CTSB, MMP9, CAII) indicated that the antler has higher bone remodeling activity. Given the up-regulated expression of immune-related molecules (S100A7, CATHL7, LTF, AZU1, ELANE and MPO), we speculate that the local immune system may contribute to the ossification of antler tip. In conclusion, proteomics technology was used to deeply analyze the protein expression patterns of antler at different development stages. This provides a strong support for the research on the molecular regulation mechanism of rapid growth and ossification of velvet antler.

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Section: Introductionmentioning

confidence: 99%

Comparative antler proteome of sika deer from different developmental stages

Zhang

Xing

2021

Sci Rep

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“…IHH negatively regulates terminal chondrocyte differentiation through Pthrpr (Kobayashi et al, 2002). IHH regulates the differentiation of mesenchymal cells into precartilage in the process of antler development (Baojin et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Whole Transcriptome Analysis of Mesenchyme Tissue in Sika Deer Antler Revealed the CeRNAs Regulatory Network Associated With Antler Development

Han

Wang

et al. 2020

Front. Genet.

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Deer antler is the only completely regenerable organ in mammals. During the rapid growth period, the antler proliferates even faster than cancerous tissue growth. However, the proliferation and development of antler have been in a stable and controllable growth cycle. In this study, we analyzed the time series expression data of nine samples from mesenchyme layer in three male sika deer in the early period of the antler with a saddle-like appearance (30 days), the rapid growth period of the antler with two branches (60 days), and the final period of the antler with three branches (90 days). Whole Transcriptome sequencing results show that in the 30 d versus 60 d group, 1,464 genes, 85 long noncoding RNAs (lncRNAs), and 61 miRNAs were identified as differentially expressed; 1,748 genes, 138 lncRNAs, and 78 miRNAs were identified as differentially expressed in 30d versus 90d group; and 816 differentially expressed genes (DEGs), 49 differentially expressed lncRNAs (DE lncRNAs), and 24 differentially expressed miRNA (DE miRNAs) were identified in 60d versus 90d group. A total of 182 miRNA-mRNA interaction pairs and 89 miRNA-lncRNA interaction pairs were screened from DEGs, DE miRNAs, and DE lncRNAs to construct the ceRNA regulatory network (ceRNET). In summary, we identified candidate mRNAs, miRNAs and lncRNAs that regulate the development of antler tip. It may lay the foundation for further investigating the molecular mechanism of antler rapid growth and development.

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“…A growing number of studies have suggested that traditional Chinese medicines exert beneficial effects via modulation of the transcription of related genes . To date, several genes have been reported to be the downstream effectors of schizandrin, including hefA and thymic stromal lymphopoietin .…”

Section: Discussionmentioning

confidence: 99%

Schizandrin protects H9c2 cells against lipopolysaccharide‐induced injury by downregulating Smad3

Zhang

Zhao²,

Bai³

et al. 2019

J Biochem & Molecular Tox

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Schizandrin is a major bioactive constituent of Schisandra chinensis (Turcz.) Baill with antioxidant and anti-inflammatory properties. The objective of this study was to explore the potential effects of schizandrin on a cell model of myocarditis. The H9c2 cells were treated with schizandrin alone or in combination with lipopolysaccharide (LPS), after which, cell survival, migration, and the release of inflammatory cytokines were assessed. Moreover, downstream effectors and signaling pathways were studied to reveal the possible underlying mechanism. As a result, LPS stimulation induced significant cell damage as cell viability was repressed and the apoptosis was induced.In the meantime, LPS promoted the release of proinflammatory cytokines including interleukin 1β (IL-1β), IL-8, IL-6, and tumor necrosis factor (TNF-α) while repressing the release of the anti-inflammatory cytokine IL-10. Schizandrin could promote H9c2 cell migration and long-term treatment (7 days) enhanced cell viability. More interestingly, pretreatment with schizandrin attenuated LPS-induced cell loss and inflammatory response. Besides this, Smad3 was a downstream effector of schizandrin. The beneficial effects of schizandrin on the H9c2 cells were attenuated when Smad3 was overexpressed. Moreover, the silencing of Smad3 deactivated c-Jun N-terminal kinase (JNK) and nuclear factor κB (NF-κB) pathways. This study preliminarily demonstrated that schizandrin prevented LPS-induced injury in the H9c2 cells and promoted the recovery of myocardial tissues by enhancing cell viability and migration. Schizandrin conferred its beneficial effects possibly by downregulating Smad3 and inhibiting the activation of JNK and NF-κB pathways. K E Y W O R D S H9c2 cell, lipopolysaccharide, myocarditis, schizandrin, Smad3

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Global analysis of tissue-differential gene expression patterns and functional regulation of rapid antler growth

Cited by 11 publications

References 62 publications

Comparative antler proteome of sika deer from different developmental stages

Comparative antler proteome of sika deer from different developmental stages

Whole Transcriptome Analysis of Mesenchyme Tissue in Sika Deer Antler Revealed the CeRNAs Regulatory Network Associated With Antler Development

Schizandrin protects H9c2 cells against lipopolysaccharide‐induced injury by downregulating Smad3

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