Global assessment of the response to chronic stress in European sea bass

Sadoul, Bastien; Alfonso, Sébastien; Cousin, Xavier; Prunet, Patrick; Bégout, Marie‐Laure; Leguen, Isabelle

doi:10.1016/j.aquaculture.2021.737072

Cited by 18 publications

(16 citation statements)

References 110 publications

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“…Major advances on the establishment of OWIs have been achieved in Atlantic salmon (Noble et al, 2018;Rey et al, 2019), trout (Noble et al, 2020), and tilapia (Pedrazzani et al, 2020). Less standardized are the welfare assessment protocols in other farmed fish, such as gilthead sea bream and European sea bass (Papaharisis et al, 2019;Sadoul et al, 2021), though specific key performance indicator-based benchmarking systems have been recently validated within the framework of MedAID 1 and PerformFISH 2 H2020 EU projects. All this will contribute to ensure that fish welfare is properly assessed in different aquaculture production systems to warrant that currently cultured fish strains are not far from their optimum welfare (Saraiva et al, 2018;Saraiva and Arechavala-Lopez, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, selective breeding of more social animals resulted in the improved behavior of the offspring (de Goede et al, 2013;Balasch and Tort, 2019). The challenge is that very often behavioral indicators are difficult to quantify in the aquatic environment (absolute changes in swimming speed, aggression levels, and gill beat frequency) and proper assessment of fish welfare often requires additional physiological measurements (Sadoul et al, 2021). Moreover, these analysis becomes tedious and laborious, though underwater cameras and echo sounder technology are relatively inexpensive and provide the opportunity for real time observation of fish behavior and intelligent feeding (Hung et al, 2016;Li et al, 2020;An et al, 2021;Georgopoulou et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Revising the Impact and Prospects of Activity and Ventilation Rate Bio-Loggers for Tracking Welfare and Fish-Environment Interactions in Salmonids and Mediterranean Farmed Fish

et al. 2022

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Behavioral parameters are reliable and useful operational welfare indicators that yield information on fish health and welfare status in aquaculture. However, aquatic environment is still constraining for some solutions based on underwater cameras or echo sounder transmitters. Thus, the use of bio-loggers internally or externally attached to sentinel fish emerges as a solution for fish welfare monitoring in tanks- and sea cages-rearing systems. This review is focused on the recently developed AEFishBIT, a small and light data storage tag designed to be externally attached to fish operculum for individual and simultaneous monitoring of swimming activity and ventilation rates under steady and unsteady swimming conditions for short-term periods. AEFishBIT is a tri-axial accelerometer with a frequency sampling of 50–100 Hz that is able to provide proxy measurements of physical and metabolic activities validated by video recording, exercise tests in swim tunnel respirometers, and differential operculum and body tail movements across fish species with differences in swimming capabilities. Tagging procedures based on tag piercing and surgery procedures are adapted to species anatomical head and operculum features, which allowed trained operators to quickly complete the tagging procedure with a fast post-tagging recovery of just 2.5–7 h in both salmonid (rainbow trout, Atlantic salmon) and non-salmonid (gilthead sea bream, European sea bass) farmed fish. Dual recorded data are processed by on-board algorithms, providing valuable information on adaptive behavior through the productive cycle with the changing environment and genetics. Such biosensing approach also provides valuable information on social behavior in terms of adaptive capacities or changes in daily or seasonal activity, linking respiratory rates with changes in metabolic rates and energy partitioning between growth and physical activity. At short-term, upcoming improvements in device design and accompanying software are envisaged, including energy-harvesting techniques aimed to prolong the battery life and the addition of a gyroscope for the estimation of the spatial distribution of fish movements. Altogether, the measured features of AEFishBIT will assist researchers, fish farmers and breeders to establish stricter welfare criteria, suitable feeding strategies, and to produce more robust and efficient fish in a changing environment, helping to improve fish management and aquaculture profitability.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Revising the Impact and Prospects of Activity and Ventilation Rate Bio-Loggers for Tracking Welfare and Fish-Environment Interactions in Salmonids and Mediterranean Farmed Fish

et al. 2022

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“…Stress is known to affect behaviour, including risk-taking [41,42]. Behavioural measures are also generally considered as reliable markers of welfare [29,43,44]. Nevertheless, to our knowledge, no previous studies have investigated long-term post-stress differences in risk-taking behaviour in fishes; behavioural differences being observed only during the stress protocol or right after.…”

Section: Discussionmentioning

confidence: 99%

“…They were dispatched in three 1.5 m 3 tanks and supplied with water at a constant temperature of 21°C. At 255 ± 2 dpf and 358 ± 2 dpf, a total of 288 and 492 fish respectively were randomly extracted from the tank for two other studies, one being published [29]. From tagging to the end of the experiment (at 462 dpf), 119 (out of 2053, 5.8%) fish died of unknown reasons.…”

Section: Methodsmentioning

confidence: 99%

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Transcriptomic profiles of consistent risk-taking behaviour across time and contexts in European sea bass

et al. 2022

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Bolder individuals have greater access to food sources and reproductive partners but are also at increased risk of predation. Boldness is believed to be consistent across time and contexts, but few studies have investigated the stability of this trait across variable environments, such as varying stress loads or long periods of time. Moreover, the underlying molecular components of boldness are poorly studied. Here, we report that boldness of 1154 European sea bass, evaluated using group risk-taking tests, is consistent over seven months and for individuals subjected to multiple environments, including a chronically stressful environment. Differences in risk-taking behaviour were further supported by differences observed in the responses to a novel environment test: shy individuals displayed more group dispersion, more thigmotaxic behaviour and lower activity levels. Transcriptomic analyses performed on extreme phenotypes revealed that bold individuals display greater expression for genes involved in social and exploration behaviours, and memory in the pituitary, and genes involved in immunity and responses to stimuli in the head kidney. This study demonstrates that personality traits come with an underpinning molecular signature, especially in organs involved in the endocrine and immune systems. As such, our results help to depict state–behaviour feedback mechanisms, previously proposed as key in shaping animal personality.

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Circulating MicroRNAs Indicative of Sex and Stress in the European Seabass (Dicentrarchus labrax): Toward the Identification of New Biomarkers

Houdelet,

Blondeau-Bidet,

Estevez-Villar

et al. 2023

Mar Biotechnol

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MicroRNAs (miRNAs) constitute a new category of biomarkers. Studies on miRNAs in non-mammalian species have drastically increased in the last few years. Here, we explored the use of miRNAs as potential, poorly-invasive markers, to identify sex and characterize acute stress in fish. The European seabass (Dicentrarchus labrax) was chosen as model because of its rapid response to stress and its specific sex determination system, devoid of sexual chromosomes. We performed a small RNA-sequencing analysis in the blood plasma of males and females' European seabass (mature and immature) as well as in the blood plasma of juveniles submitted to an acute stress and sampled throughout the recovery period (at 0h, 0.5h, 1.5h and 6h). In immature individuals, both miR-1388-3p and miR-7132a-5p were up-regulated in females, while miR-499a-5p was more abundant in males. However, no miRNAs were found to be differentially expressed between sexes in the blood plasma of mature individuals. For the acute stress analysis, five miRNAs (miR-155-5p, miR-200a-3p, miR-205-1-5p, miR-143-3p and miR-223-3p) followed cortisol production over time. All miRNAs identified were tested and validated by RT-qPCR on sequenced samples.A complementary analysis on the 3'UTR sequences of the European seabass allowed to predict potential mRNA targets, some of them being particularly relevant regarding stress regulation, e.g. the glucocorticoid and the mineralocorticoid receptor. The present study provides new avenues and recommendations on the use of miRNAs as biomarkers of sex or stress of the European seabass, with potential application on other fish species.

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Global assessment of the response to chronic stress in European sea bass

Cited by 18 publications

References 110 publications

Revising the Impact and Prospects of Activity and Ventilation Rate Bio-Loggers for Tracking Welfare and Fish-Environment Interactions in Salmonids and Mediterranean Farmed Fish

Revising the Impact and Prospects of Activity and Ventilation Rate Bio-Loggers for Tracking Welfare and Fish-Environment Interactions in Salmonids and Mediterranean Farmed Fish

Transcriptomic profiles of consistent risk-taking behaviour across time and contexts in European sea bass

Circulating MicroRNAs Indicative of Sex and Stress in the European Seabass (Dicentrarchus labrax): Toward the Identification of New Biomarkers

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